Thymoquinone: fifty years of success in the battle against cancer models

Schneider‐Stock, Regine; Fakhoury, Isabelle; Zaki, Angela M.; El-Baba, Chirine; Gali‐Muhtasib, Hala

doi:10.1016/j.drudis.2013.08.021

Cited by 192 publications

(155 citation statements)

References 82 publications

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“…Traditional medicine has taken advantage of the anti-inflammatory, antioxidant and anticarcinogenic properties associated with TQ, which supports the hypothesis of TQ being a promising dietary chemopreventive agent (6). In the previous decade, the antitumor activity of TQ has been investigated in a number of studies (6)(7)(8). TQ was observed to induce antitumor effects in several types of cancer, including breast (9), lung (10), multiple myeloma (11), pancreatic (12), cervical (13), colon (14) and prostate cancer (15), as well as squamous (16) and hepatocellular carcinoma (17), acute lymphoblastic leukemia (18), glioblastoma (19), osteosarcoma (20), neuroblastoma (21), bladder (22), gastric (23) and ovarian cancer (24).…”

Section: Introductionmentioning

confidence: 93%

“…Nigella sativa is an annual herbaceous plant belonging to the Ranunculaceae family, which has been commonly used in traditional Middle Eastern folk medicine as a natural remedy for various ailments for >2,000 years (8). Nigella sativa is additionally used as a food additive and flavoring in numerous countries (8).…”

Section: Discussionmentioning

confidence: 99%

“…Nigella sativa is additionally used as a food additive and flavoring in numerous countries (8). TQ, or 2-isopropyl-5-methyl-1,4-benzoquinone, is a Nigella sativa essential oil that is known to be the principal active compound of the seed, and is responsible for a number of its antioxidant and anti-inflammatory effects (6,8).…”

Section: Discussionmentioning

confidence: 99%

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Thymoquinone chemosensitizes colon cancer cells through inhibition of NF-κB

2016

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Abstract. In the present study, the effects and molecular mechanisms of thymoquinone (TQ) on colon cancer cells were investigated. Cell viability was determined using a Cell Counting Kit-8 assay, and the results revealed that treatment with TQ significantly decreased cell viability in COLO205 and HCT116 cells in a dose-dependent manner. TQ treatment additionally sensitized COLO205 and HCT116 cells to cisplatin therapy in a concentration-dependent manner. To investigate the molecular mechanisms of TQ action, western blot analysis was used to determine the levels of phosphorylated p65 and nuclear factor-κB (NF-κB)-regulated gene products vascular endothelial growth factor (VEGF), c-Myc and B-cell lymphoma 2 (Bcl-2). The results indicated that TQ treatment significantly decreased the level of phosphorylated p65 in the nucleus, which indicated the inhibition of NF-κB activation by TQ treatment. Treatment with TQ also decreased the expression levels of VEGF, c-Myc and Bcl-2. In addition, the inhibition of NF-κB activation with a specific inhibitor, pyrrolidine dithiocarbamate, potentiated the induction of cell death and caused a chemosensitization effect of TQ in colon cancer cells. Overall, the results of the present study suggested that TQ induced cell death and chemosensitized colon cancer cells by inhibiting NF-κB signaling.

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Section: Introductionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Thymoquinone chemosensitizes colon cancer cells through inhibition of NF-κB

2016

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“…So the heat generated during the extraction of SPO (~50 °C) can cause melting of the crystals of this compound and facilitate its co-extraction with the crude oil leading to a higher percentage in the volatile fraction of SPO. One should bear in mind that TQ is the compound that gives the volatile oil and consequently its parent crude oil their functionality due to the pharmaceutical and therapeutic properties of this compound (Gülşen et al, 2016;Darakhshan et al, 2015;Schneider-Stock et al, 2014). From the above mentioned, it could be presumed that the high yield of the volatile oil fraction (2.6%) along with its high content of TQ (68.13%) of the SPO can be considered contributing factors that justify the high oxidative stability and relatively lower PV of that oil, as mentioned in the previous sections.…”

Section: Discussionmentioning

confidence: 99%

Potentials of enhancing the physicochemical and functional characteristics of <em>Nigella sativa</em> oil by using the screw pressing technique for extraction

Hamed¹,

Shaaban²,

Ramadan³

et al. 2017

Grasas y Aceites

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SUMMARY:In the current investigation the crude oil of Nigella sativa was extracted from seeds using hydraulic and screw pressing techniques. Different parameters were evaluated in order to find out the appropriate technique to enhance the physicochemical and functional-related characteristics of the extracted crude oil. Results showed that the acid and peroxide values were significantly lower in the screw pressed oil (SPO) than in the hydraulic pressed oil (HPO). The total phenolic content of the SPO was significantly higher than that of HPO. Evaluation of the oxidative stability using the Rancimat test showed that SPO recorded a much higher oxidative stability index (40.07 h) than HPO (0.51 h). The yield of the volatile oil fraction and its contents of thymoquinone isolated from the SPO were higher than that from the HPO. Biological evaluation revealed that the SPO had significantly higher antimicrobial activity than HPO against Listeria monocytogenes and Staphylococcus aureus at 40 µL/well. KEYWORDS : Crude oil; Functionality; Hydraulic pressing; Nigella sativa; Screw pressing RESUMEN: Mejoras potenciales de las características físico-químicas y funcionales del aceite de Nigella sativa mediante extracción con prensa de tornillo. En la presente investigación se extrajo el aceite crudo de Nigella sativa de las semillas utilizando técnicas hidráulicas y de prensado de tornillo. Se evaluaron diferentes parámetros para conocer la técnica apropiada que potencie las características fisicoquímicas y funcionales del aceite extraído. Los resultados mostraron que los valores de ácido y peróxido fueron significativamente más bajos en el aceite de presión con tornillo (SPO) que en el aceite prensado hidráulico (HPO). El contenido fenólico total del SPO fue significativamente mayor que el de HPO. La evaluación de la estabilidad oxidativa mediante Rancimat demostró que el aceite SPO tiene un índice de estabilidad oxidativa mucho más alto (40,07 h) que el HPO (0,51 h). El rendimiento de la fracción volátil y su contenido de timoquinona aislada del aceite SPO fueron superiores a los del HPO. La evaluación biológica reveló que el aceite SPO tenía una actividad antimicrobiana significativamente mayor que el HPO contra Listeria monocitogenes y Staphylococcus aureus a 40 μL/pocillo.

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“…7 Moreover, many studies have shown that the growth inhibitory effect of TQ is specific to cancer cells while it is minimally toxic to normal cells. 8,9 Although TQ has tremendous potential as a therapeutic compound for cancer, the effectiveness and oral bioavailability are limited by its lipophilicity and poor solubility in water. 2,10 Similar limitation has also occurred in cancer therapeutic drugs, such as doxorubicin, paclitaxel, and vincristine.…”

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confidence: 99%