Thymoquinone counteracts oxidative and inflammatory machinery in carrageenan-induced murine paw edema model

Hijazy, Hayfa Hussin Ali; Dahran, Naief; Althagafi, Hussam A.; Alharthi, Fahad; Habotta, Ola A.; Oyouni, Atif Abdulwahab A.; Algahtani, Mohammad; Theyab, Abdulrahman; Al‐Amer, Osama; Lokman, Maha S.; Alsharif, Khalaf F.; Albrakati, Ashraf; Amin, Hatem K.; Dawood, Shauq Mumtaz; Kassab, Rami B.; Ellethy, Rania A.

doi:10.1007/s11356-022-23343-8

Cited by 6 publications

(3 citation statements)

References 60 publications

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“…In recent research conducted by Hijazy et al [17], the effects of oral administration on the development of edema, oxidative stress, and inflammation in mice that had paw edema were investigated. Thymoquinone has been shown to reduce pro-inflammatory mediators in inflamed paw tissue, including IL-1, TNF-α, IL-6, MCP-1, C-reactive protein, myeloperoxidase, and NF-κB.…”

Section: Discussionmentioning

confidence: 99%

Effect of Nigella sativa Versus Wheat Germ Oil on the Healing of Traumatic Ulcer in Albino Rats

Mohamed,

ElBaz,

Hegazy

et al. 2024

Cureus

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Background and objective: Nigella sativa (NS) oil has been used as an ointment for relief from abscesses, nasal ulcers, orchitis, eczema, and swollen joints. The nutritional and biological values of wheat germ oil (WGO) are imperative points for testing its wound healing properties in traumatic ulcer. The aim of the study was to evaluate and compare the ability of NS versus WGO in promoting the healing of induced traumatic ulcer in albino rats clinically and histologically.Materials and methods: This study was carried out after the approval of the Research Ethics Committee (REC) of the Faculty of Dentistry, Suez Canal University, in Ismailia, Egypt, on 60 albino rats with induced labial ulcer according to calculated sample size. All animals were anaesthetized with an intraperitoneal injection of 10% ketamine. The ulcer was produced on the labial mucosa corresponding to the midline between the lower two incisors of each rat. After induction of the ulcer, rats were randomly divided into four groups according to the treatment medicament: Group A (negative control group): 15 rats which remained without treatment; Group B (positive control): 15 rats which received daily a topical application of 1 ml of cetylpyridinium chloride (CPC) and lidocaine gel; Group C (NS group): 15 rats which received a daily topical application of 1 mm of NS oil painted by a brush covering the whole area of the ulcer; and Group D (WGO group): 15 rats which received 1 mm of WGO. The ulcers were measured using a digital caliper and were recorded using a digital camera at days 0, 3, 7, and 9, the largest (D) and smallest (d) diameters of the lesion were recorded, and the ulcer area was calculated using the following formula: A=π×D/2×d/2. Tissue samples were taken for histological examination, and the labial mucosa was dissected out and embedded in paraffin wax blocks. The blocks were cut with microtome to obtain sections of 4-5 μm thickness to be stained with hematoxylin and eosin stain and Masson's trichrome stain. All sections were examined under a light microscope, and the presence of inflammatory cells and collagen tissue remodeling were evaluated.Results: Within the control group, there are statistically non-significant changes in the mean of the surface area of ulcer when comparing changes in 10 rats who survived till the seventh day and inflammatory cell count when comparing changes in five rats who were sacrificed at the seventh day. There was a significant decrease in surface area and inflammatory cell count in five rats who survived till the ninth day. Within the WGO group only, all survived rats had healed ulcer at the ninth day. There is a significant decrease in inflammatory cell count in five rats who survived till the ninth day.Conclusion: WGO was significantly more effective in the treatment of animal-induced ulcer compared to NS oil or CPC and lidocaine oral gel.

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Section: Discussionmentioning

confidence: 99%

Effect of Nigella sativa Versus Wheat Germ Oil on the Healing of Traumatic Ulcer in Albino Rats

Mohamed,

ElBaz,

Hegazy

et al. 2024

Cureus

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“…However, NO interacts with superoxide radicals at high concentrations, forming a powerful oxidant molecule, peroxynitrite, which causes severe cellular impairments. [39,40] The depleted endogenous antioxidants associated with MI induction may be due to their exhaustion and utilization for quenching ROS produced in response to ISO injection. [41] Significantly, PCA treatment inhibited the development of oxidative stress in the myocardium and decreased MDA, NO, and downregulated mRNA expression of nitric oxide synthase 2.…”

Section: Discussionmentioning

confidence: 99%

Protocatechuic acid reverses myocardial infarction mediated by β‐adrenergic agonist via regulation of Nrf2/HO‐1 pathway, inflammatory, apoptotic, and fibrotic events

Zhang

et al. 2023

J Biochem & Molecular Tox

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Myocardial infarction (MI) is an instant ischemic death of cardiomyocytes that remains a major global cause of mortalities. MI is accompanied by oxidative, inflammatory, apoptotic, and fibrotic insults. Protocatechuic acid (PCA) is a polyphenolic compound with various potent biological activities. In this study, we explored the possible cardioprotective role of PCA against isoproterenol (ISO)-mediated MI. Rats were either injected with ISO (85 mg/kg, subcutaneously) or pretreated with PCA (100 or 200 mg/kg, orally). PCA supplementation markedly normalized ISO-induced disturbed cardiac function markers (creatine kinase-MB, lactate dehydrogenase, and troponin T). Notably, PCA administration exerted remarkable increases in glutathione and its derived enzymes, superoxide dismutase, and catalase, as well as decreases in malondialdehyde and nitric oxide levels in the injured cardiac tissue. The molecular findings validated the augmented cellular antioxidative capacity by PCA via increasing the gene expressions of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1. The cardioprotective efficacy of PCA extended to suppress cardiac inflammation as demonstrated by the decreased levels of tumor necrosis factor-alpha, interleukin-1 beta, and nuclear factor kappa B. Additionally, PCA prevented cardiomyocyte loss and fibrosis by decreasing Bax, caspase-3, transforming growth factor-β1 and matrix metalloproteinase-9, and enhancing B-cell

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“…In recent research conducted by Hijazy et al [91], the effects of oral administration on the development of edema, oxidative stress, and inflammation in mice that had paw edema were investigated. The study found that thymoquinone reduced paw edema volume over time, lessened writhing movements caused by acetic acid, and decreased ear edema triggered by xylene.…”

Section: Thymoquinone's Effects On Inflammation and Immune System Mod...mentioning

confidence: 99%

How Thymoquinone from Nigella sativa Accelerates Wound Healing through Multiple Mechanisms and Targets

Kmail,

Said,

Saad

2023

CIMB

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Wound healing is a multifaceted process necessitating the collaboration of numerous elements to mend damaged tissue. Plant and animal-derived natural compounds have been utilized for wound treatment over the centuries, with many scientific investigations examining these compounds. Those with antioxidant, anti-inflammatory, and antibacterial properties are particularly noteworthy, as they target various wound-healing stages to expedite recovery. Thymoquinone, derived from Nigella sativa (N. sativa)—a medicinal herb with a long history of use in traditional medicine systems such as Unani, Ayurveda, Chinese, and Greco-Arabic and Islamic medicine—has demonstrated a range of therapeutic properties. Thymoquinone exhibits antimicrobial, anti-inflammatory, and antineoplastic activities, positioning it as a potential remedy for skin pathologies. This review examines recent research on how thymoquinone accelerates wound healing and the mechanisms behind its effectiveness. We carried out a comprehensive review of literature and electronic databases, including Google Scholar, PubMed, Science Direct, and MedlinePlus. Our aim was to gather relevant papers published between 2015 and August 2023. The main criteria for inclusion were that the articles had to be peer reviewed, original, written in English, and discuss the wound-healing parameters of thymoquinone in wound repair. Our review focused on the effects of thymoquinone on the cellular and molecular mechanisms involved in wound healing. We also examined the role of cytokines, signal transduction cascades, and clinical trials. We found sufficient evidence to support the effectiveness of thymoquinone in promoting wound healing. However, there is no consensus on the most effective concentrations of these substances. It is therefore essential to determine the optimal treatment doses and the best route of administration. Further research is also needed to investigate potential side effects and the performance of thymoquinone in clinical trials.

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Thymoquinone counteracts oxidative and inflammatory machinery in carrageenan-induced murine paw edema model

Cited by 6 publications

References 60 publications

Effect of Nigella sativa Versus Wheat Germ Oil on the Healing of Traumatic Ulcer in Albino Rats

Effect of Nigella sativa Versus Wheat Germ Oil on the Healing of Traumatic Ulcer in Albino Rats

Protocatechuic acid reverses myocardial infarction mediated by β‐adrenergic agonist via regulation of Nrf2/HO‐1 pathway, inflammatory, apoptotic, and fibrotic events

How Thymoquinone from Nigella sativa Accelerates Wound Healing through Multiple Mechanisms and Targets

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