Thymoquinone, an active constituent of Nigella sativa seeds, binds with bilirubin and protects mice from hyperbilirubinemia and cyclophosphamide-induced hepatotoxicity

Laskar, Amaj Ahmed; Khan, Masood Alam; Rahmani, Arshad Husain; Fatima, Sana; Younus, Hina

doi:10.1016/j.biochi.2016.05.020

Cited by 50 publications

(35 citation statements)

References 30 publications

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“…On day 5 posttreatment, the levels of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) were determined in the spleen tissue homogenates as described earlier [ 25 ]. The spleen tissues from untreated or treated groups of mice were rinsed in cold phosphate-buffered saline (PBS) and the connective tissue was removed.…”

Section: Methodsmentioning

confidence: 99%

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Immune-Stimulatory and Therapeutic Activity of Tinospora cordifolia: Double-Edged Sword against Salmonellosis

Alsuhaibani

Khan

2017

Journal of Immunology Research

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The present study was aimed at determining the activity of aqueous and methanolic extracts of Tinospora cordifolia (AETC and METC) against Salmonella typhimurium. In vitro anti-Salmonella activity of T. cordifolia was determined through the broth dilution and agar well diffusion assays. The immune-stimulating potential of AETC or METC was determined by measuring the cytokine levels in the culture supernatants of treated murine J774 macrophages. Antibacterial activity of AETC or METC was determined by treating S. typhimurium-infected macrophages and BALB/C mice. The toxicity of AETC or METC was determined by measuring the levels of liver inflammation markers aspartate transaminase (AST) and alanine transaminase (ALT) and antioxidant enzymes. Macrophages treated with AETC or METC secreted greater levels of IFN-γ, TNF-α, and IL-1β. METC showed greater activity against S. typhimurium infection in macrophages and mice as well. Treatment with METC resulted in increased survival and reduced bacterial load in S. typhimurium-infected mice. Moreover, METC or AETC treatment reduced the liver inflammation and rescued the levels of antioxidant enzymes in S. typhimurium-infected mice. The results of the present study suggest that the use of T. cordifolia may act as a double-edged sword in combating salmonellosis.

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Section: Methodsmentioning

confidence: 99%

“…To determine the liver toxicity, the levels of aspartate transaminase (AST) and alanine transaminase (ALT), the markers of liver inflammation, were determined in the blood of untreated or treated groups of mice [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Immune-Stimulatory and Therapeutic Activity of Tinospora cordifolia: Double-Edged Sword against Salmonellosis

Alsuhaibani

Khan

2017

Journal of Immunology Research

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“…In the same study, they did not observe any hepatotoxic effects when applying lower doses (4, 8, 12.5, 25 and 50 mg/kg) TQ. ALT, AST and lactate dehydrogenase (LDH) levels were not significantly elevated upon treatment [ 39 , 40 , 41 ]. This was confirmed in many other studies.…”

Section: Introductionmentioning

confidence: 99%

“…This was confirmed in many other studies. Intraperitoneal TQ doses between 5 to 18 mg/kg were injected to mice and rats without side effects [ 40 , 41 , 42 , 43 , 44 , 45 ]. However, according to Mansour et al, the only dose that ameliorated hepatotoxicity of CCI4 was 12.5 mg/kg ip and side effects were reported at doses higher than 50 mg/kg ip (see Section 2.2 ) [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Plants and Surgery: The Protective Effects of Thymoquinone on Hepatic Injury—A Systematic Review of In Vivo Studies

Tekbaş

Huebner

Settmacher

et al. 2018

IJMS

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Multimodal treatment concepts including liver transplantation for hepatocellular carcinoma (HCC), extended resection methods and neoadjuvant chemotherapy for colorectal liver metastasis significantly improve patients’ outcome. However, surgery-induced hepatic ischemia-reperfusion injury (IRI) and chemotherapy-associated hepatotoxicity result in hepatocellular damage and compromised liver function. Activation of common key pathways in ischemic liver and hepatotoxic injury results in oxidative stress, inflammatory responses and apoptosis causing organ damage. Controlling liver damage before and during surgery is essential for the postoperative outcome. Nigella sativa has a long tradition as a natural remedy. In the essential oil, Thymoquinone (TQ) was identified as the main component and responsible for most of the therapeutic effects. Therefore, this systematic review aimed to summarize the hepatoprotective effects of TQ and its potential suitability to improve surgical outcome by reducing surgical ischemic injury and hepatotoxicity of neoadjuvant chemotherapy. The key findings can be summarized as TQ having strong antioxidant, anti-inflammatory, antifibrotic, anti-/proapoptotic and anticarcinogenic effects. Almost no side effects were reported irrespective of a large dose range, suggesting a wide therapeutic window. These results give rise to the expectation that TQ could evolve to a novel powerful drug to reduce hepatic injury.

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“…cancer activity 5 , anti-antioxidant activity 6 , antiinflammatory 7 , anti-diabetic and anti-glycating property 8,9,10 , antimicrobial, antiepileptic and hepatoprotective activity 11,12,13 , potential remedial action on dental and oral illness 14 , protection against cardiovascular health problem 15 , a potentate drug against neurogenerative disorders and brain damage 16 , a remedy for renal diseases including nephrolithiasis and renal damage 17 , protection against kidney damage due to morphine toxicity 18 and as a curative drug for stress-induced gastric ulcers 19 , have been reported for this medicinal plant. The objective of this study is to screen the phytochemicals and to evaluate the antibacterial potential of Nigella sativa L. seeds against ESBL positive Escherichia coli isolates.…”

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confidence: 99%

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2019

IJPSR

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Thymoquinone, an active constituent of Nigella sativa seeds, binds with bilirubin and protects mice from hyperbilirubinemia and cyclophosphamide-induced hepatotoxicity

Cited by 50 publications

References 30 publications

Immune-Stimulatory and Therapeutic Activity of Tinospora cordifolia: Double-Edged Sword against Salmonellosis

Immune-Stimulatory and Therapeutic Activity of Tinospora cordifolia: Double-Edged Sword against Salmonellosis

Plants and Surgery: The Protective Effects of Thymoquinone on Hepatic Injury—A Systematic Review of In Vivo Studies

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