Thymine-modified chitosan with broad-spectrum antimicrobial activities for wound healing

Deng, Pengpeng; Chen, Juanjuan; Yao, Lichao; Zhang, Pingan; Zhou, Jinping

doi:10.1016/j.carbpol.2021.117630

Cited by 42 publications

(15 citation statements)

References 58 publications

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“…Immunohistochemistry analysis was conducted as previously described [ 42 ]. Briefly, wound tissue sections were deparaffinized, rehydrated, and boiled in a 100 °C citrate buffer bath.…”

Section: Methodsmentioning

confidence: 99%

Phloroglucinol Derivative Carbomer Hydrogel Accelerates MRSA-Infected Wounds’ Healing

Huang

Yang

Zhang

et al. 2022

IJMS

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Globally, wound infection is considered to be one of the major healthcare problems, with bacterial infections being the most critical threat, leading to poor and delayed wound healing, and even death. As a superbug, methicillin-resistant Staphylococcus aureus (MRSA) causes a profound hazard to public health safety, prompting us to search for alternative treatment approaches. Herein, the MTT test and Hoechst/propidium iodide (PI) staining demonstrated that PD was slightly less toxic to human fibroblasts including Human keratinocytes (HaCaT) cell line than Silver sulfadiazine (SSD), and Vancomycin (Van). In the MRSA-infected wound model, PD hydrogel (1%, 2.5%) was applied with for 14 days. The wound healing of PD hydrogel groups was superior to the SSD, Van, and control groups. Remarkably, the experimental results showed that PD reduced the number of skin bacteria, reduced inflammation, and upregulated the expression of PCNA (keratinocyte proliferation marker) and CD31 (angiogenesis manufacturer) at the wound site by histology (including hematoxylin–eosin (HE) staining, Masson staining) and immunohistochemistry. Additionally, no toxicity, hemocompatibility or histopathological changes to organs were observed. Altogether, these results suggested the potential of PD hydrogel as a safe, effective, and low toxicity hydrogel for the future clinical treatment of MRSA-infected wounds.

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“…Immunohistochemistry analysis was conducted as previously described [ 42 ]. Briefly, wound tissue sections were deparaffinized, rehydrated, and boiled in a 100 °C citrate buffer bath.…”

Section: Methodsmentioning

confidence: 99%

Phloroglucinol Derivative Carbomer Hydrogel Accelerates MRSA-Infected Wounds’ Healing

Huang

Yang

Zhang

et al. 2022

IJMS

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“…Due to physical injury or chronic diseases, skin damage happens commonly in daily life. Compared with physical injury, wounds caused by chronic diseases exhibit a long-term and difficult recovery process. − Nonhealing chronic wounds can cause severe complications such as bacterial infections and bleeding, posing a great threat to patients’ health. − Conventional wound dressings, including gauze and hydrogels, have been widely used to treat chronic wounds. − Despite their success in preventing chronic wounds from aggravation, some disadvantages including uncontrollable drug release, adhesion to wound tissues, and single function hinder their further application in complete wound management (healing and monitoring). To address these problems, considerable efforts have been devoted to explore wound dressings with versatile functions and controllable drug release. − Among them, microneedle (MN) patches have shown significant potentials in building multifunctional dressing for intelligent wound management. − With microscale (usually <1 mm) needles, MN patches can penetrate skin without reaching nerves and blood vessels, achieving minimally invasive drug delivery.…”

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confidence: 99%

Shark Tooth-Inspired Microneedle Dressing for Intelligent Wound Management

et al. 2021

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Intelligent management beyond therapeutic drug treating holds significant prospects in facilitating the recovery of intractable chronic wounds. Here, inspired by the flat and inclined structure of shark teeth, we present a shark tooth-inspired microneedle patch for intelligent wound management. By simply replicating negative molds fabricated by laser engraving and using origami, such a biomimetic microneedle patch can be fabricated easily and rapidly. The biomimetic structures endow the microneedle patch with stable adhesion during the long-term recovery process of chronic wounds. Porous ordered structures and a temperature-responsive hydrogel are utilized to construct a controllable drug release system on the microneedle patch. The microfluidic channel composed of microneedle arrays and porous ordered structures enables a microneedle patch with the capacity to analyze several inflammatory factors. In addition, MXene electronics was patterned on the microneedle patch in order to achieve sensitive motion monitoring. Also, it was demonstrated from in vivo diabetic rat experiments that recovery of full-thickness cutaneous wounds including stripe-shaped and circular wounds can be facilitated by employing the drug-loaded biomimetic microneedle patch.

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“…Blood vessels carry oxygen and nutrients to wound tissue and play a critical role in wound healing. , CD31 plays an active role in angiogenesis and can evaluate the regeneration of new blood vessels. , As shown in Figure a,c, the expression level of CD31 in gauze, commercial dressing, H-L-12, and H-NPs-12 groups was increasing, suggesting that the number of new blood vessels in the regenerated tissue increased successively. These results further demonstrated that the H-NPs-12 hydrogel could promote the regeneration of new blood vessels in regenerated tissues, thereby promoting wound healing.…”

Section: Resultsmentioning

confidence: 92%

Conductive, Self-Healing, Adhesive, and Antibacterial Hydrogels Based on Lignin/Cellulose for Rapid MRSA-Infected Wound Repairing

Deng

Chen

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

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The abuse of antibiotics induces the emergence of drug-resistant bacteria, which greatly increases the difficulty of clinical treatment of infected wounds. It is urgent to design a multifunctional wound dressing independent of antibiotics. In this work, we designed multifunctional hydrogels based on lignin and cellulose in natural polymers. Lignin with antioxidant properties could reduce silver nanoparticles in situ and could also be used as a crosslinking agent to construct hydrogels between hydroxypropyl cellulose modified with phenylboric acid by a dynamic borate bond. Hydrogels have excellent properties such as self-healing, shape adaptability, biocompatibility, blood compatibility, antioxidant properties, excellent broad-spectrum antimicrobial properties, good tissue adhesion, and electrical conductivity. The tissue adhesion of hydrogels endows them with an excellent hemostasis property in a rat liver injury model. In vivo experiments demonstrated that hydrogels can maintain a moist healing environment, reduce inflammatory cell infiltration, promote M2 macrophage polarization, accelerate collagen deposition, promote the regeneration of new blood vessels, and significantly speed up the wound healing of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds. Therefore, these multifunctional hydrogels are an excellent candidate to treat multiple stages of wound healing and have a broad application prospect in the medical field.

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Thymine-modified chitosan with broad-spectrum antimicrobial activities for wound healing

Cited by 42 publications

References 58 publications

Phloroglucinol Derivative Carbomer Hydrogel Accelerates MRSA-Infected Wounds’ Healing

Phloroglucinol Derivative Carbomer Hydrogel Accelerates MRSA-Infected Wounds’ Healing

Shark Tooth-Inspired Microneedle Dressing for Intelligent Wound Management

Conductive, Self-Healing, Adhesive, and Antibacterial Hydrogels Based on Lignin/Cellulose for Rapid MRSA-Infected Wound Repairing

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