Thymine glycols and pyrimidine dimers in brain DNA during post-ischemic reperfusion

O’Neil, Brian J.; Krause, Gary S.; White, Blaine C.

doi:10.1016/0300-9572(91)90077-c

Resuscitation

1991

DOI: 10.1016/0300-9572(91)90077-c

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Thymine glycols and pyrimidine dimers in brain DNA during post-ischemic reperfusion

Brian J. O’Neil

Gary S. Krause

Blaine C. White

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Cited by 6 publications

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“…R eactive oxygen intermediates (ROIs) have been implicated as inducers of ocular injury and other tissue injury in several pathological conditions, such as ischemia/reperfusion (Fukuchi et al 1991;O'Neil et al 1991;Szabo et al 1991;Faberowski et al 1989;Garcia and Anderson 1989;Rozga 1989;Siesjo 1988;Tamura et al 1988;Weiss 1986;McCord 1985;Feeney and Berman 1976;Kagan et al 1973). Adult T-cell leukemia-derived factor (ADF) is identical to human thioredoxin (Tagaya et al 1989).…”

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confidence: 98%

Analysis of Localization of Adult T-cell Leukemia-derived Factor in the Transient Ischemic Rat Retina After Treatment with OP-1206 α-CD, a Prostaglandin E₁Analogue

Yamamoto

Ohira

Honda

et al. 1997

J Histochem Cytochem.

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Prostaglandin E1 (PGE1) is commonly used in therapy for obstructive diseases, including ischemic retinopathy, in which pathogenetic reactive oxygen intermediates are responsible. However, the mechanism(s) of PGE1 in reducing tissue damage is still unclear. Adult T-cell leukemia-derived factor/human thioredoxin (ADF) is induced by oxidative stresses and has protective activity against oxidative cellular injury. To evaluate the possible involvement of ADF in the tissue-protective effect of PGE1, we analyzed ADF expression immunohistochemically using a rat transient retinal ischemia model. Rats were treated orally with 300 micrograms/kg/day OP-1206 alpha-cyclodextrin clathrate (OP-1206), a stable PGE1 analogue, for 14 days after photodynamic retinal vascular thrombosis by rose Bengal. Rats without any OP-1206 treatment were used as controls. In the OP-1206-treated rats, minimal retinal atrophy due to ischemia/reperfusion was observed histologically up to 14 days, whereas in the non-treated rats the inner layer of the retina became markedly atrophic. In parallel with the histological change, after 14 days following thrombosis ADF immunoreactivity was preserved on retinal pigment epithelial cells in the OP-1206-treated rats, whereas it was diminished in the non-treated rats. These findings suggest an important role for ADF in the OP-1206-dependent suppression of retinal tissue damage caused by oxidative insult.

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mentioning

confidence: 98%

Analysis of Localization of Adult T-cell Leukemia-derived Factor in the Transient Ischemic Rat Retina After Treatment with OP-1206 α-CD, a Prostaglandin E₁Analogue

Yamamoto

Ohira

Honda

et al. 1997

J Histochem Cytochem.

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Fluorescent histochemical localization of lipid peroxidation during brain reperfusion following cardiac arrest

et al. 1993

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Rats were subjected to cardiac arrest and resuscitation, 90 min of reperfusion, and in situ perfusion fixation. Thiobarbituric acid (TBA) was included in the aldehyde-free perfusion fixative, the TBA reaction was driven in situ by heating, and fluorescence microscopy was utilized to characterize the location of products of the TBA reaction. Absorbance-difference spectra were performed on butanol-extracted brain homogenates to confirm in situ formation of TBA adducts with aldehydic products of lipid peroxidation. Nissl-stained sections revealed good cellular fixation without shrinkage artifacts. Fluorescence was not seen microscopically when TBA was omitted from the perfusion fixative, and little fluorescence was present in normal brains or brains after ischemia only. However, after 90-min reperfusion, intense granular fluorescence was seen in the neuronal perikarya (especially at the base of the apical dendrite) of numerous pyramidal neurons in cortical layers 5 and 6 and in the pyramidal layer of Ammon's horn in the hippocampus. The nuclei of these cells exhibited no fluorescence. Fluorescence was also present in some striatal neurons, but was absent in the adjacent radial bundles. Neither glia nor white matter exhibited similar fluorescence. These observations indicate that neurons in the selectively vulnerable zones of the cortex and hippocampus are early and specific targets of lipid peroxidation during post-ischemic reperfusion.

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Brain ischemia and reperfusion: molecular mechanisms of neuronal injury

White

Sullivan

DeGracia

et al. 2000

Journal of the Neurological Sciences

723

455

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Thymine glycols and pyrimidine dimers in brain DNA during post-ischemic reperfusion

Cited by 6 publications

References 70 publications

Analysis of Localization of Adult T-cell Leukemia-derived Factor in the Transient Ischemic Rat Retina After Treatment with OP-1206 α-CD, a Prostaglandin E₁Analogue

Analysis of Localization of Adult T-cell Leukemia-derived Factor in the Transient Ischemic Rat Retina After Treatment with OP-1206 α-CD, a Prostaglandin E₁Analogue

Fluorescent histochemical localization of lipid peroxidation during brain reperfusion following cardiac arrest

Brain ischemia and reperfusion: molecular mechanisms of neuronal injury

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Thymine glycols and pyrimidine dimers in brain DNA during post-ischemic reperfusion

Cited by 6 publications

References 70 publications

Analysis of Localization of Adult T-cell Leukemia-derived Factor in the Transient Ischemic Rat Retina After Treatment with OP-1206 α-CD, a Prostaglandin E1Analogue

Analysis of Localization of Adult T-cell Leukemia-derived Factor in the Transient Ischemic Rat Retina After Treatment with OP-1206 α-CD, a Prostaglandin E1Analogue

Fluorescent histochemical localization of lipid peroxidation during brain reperfusion following cardiac arrest

Brain ischemia and reperfusion: molecular mechanisms of neuronal injury

Contact Info

Product

Resources

About

Analysis of Localization of Adult T-cell Leukemia-derived Factor in the Transient Ischemic Rat Retina After Treatment with OP-1206 α-CD, a Prostaglandin E₁Analogue

Analysis of Localization of Adult T-cell Leukemia-derived Factor in the Transient Ischemic Rat Retina After Treatment with OP-1206 α-CD, a Prostaglandin E₁Analogue