Thymic Stromal Lymphopoietin Is Produced by Dendritic Cells

Kashyap, Mohit; Rochman, Yrina; Spolski, Rosanne; Samsel, Leigh; Leonard, Warren J.

doi:10.4049/jimmunol.1100355

Cited by 128 publications

(112 citation statements)

References 37 publications

(44 reference statements)

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“…CD127 (IL7Rα) is a receptor shared between IL-7 and thymic stromal lymphopoietin (TSLP), a cytokine capable of rescuing B-cell development when overexpressed in the absence of IL-7 (33). Because TSLP is produced by dendritic cells (34), which are dramatically expanded in Flt3ltg mice (8) For each mouse genotype, mean ± SD is shown. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.…”

Section: Ly6dmentioning

confidence: 99%

Permissive roles of cytokines interleukin-7 and Flt3 ligand in mouse B-cell lineage commitment

Muenchow

Albertí-Servera

Klein

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Hematopoietic cells are continuously generated throughout life from hematopoietic stem cells, thus making hematopoiesis a favorable system to study developmental cell lineage commitment. The main factors incorporating environmental signals to developing hematopoietic cells are cytokines, which regulate commitment of hematopoietic progenitors to the different blood lineages by acting either in an instructive or a permissive manner. Fms-like tyrosine kinase-3 (Flt3) ligand (FL) and Interleukin-7 (IL-7) are cytokines pivotal for B-cell development, as manifested by the severely compromised B-cell development in their absence. However, their precise role in regulating B-cell commitment has been the subject of debate. In the present study we assessed the rescue of B-cell commitment in mice lacking IL-7 but simultaneously overexpressing FL. Results obtained demonstrate that FL overexpression in IL-7–deficient mice rescues B-cell commitment, resulting in significant Ebf1 and Pax5 expression in Ly6D+CD135+CD127+CD19− precursors and subsequent generation of normal numbers of CD19+ B-cell progenitors, therefore indicating that IL-7 can be dispensable for commitment to the B-cell lineage. Further analysis of Ly6D+CD135+CD127+CD19− progenitors in IL-7– or FL-deficient mice overexpressing Bcl2, as well as in IL-7 transgenic mice suggests that both FL and IL-7 regulate B-cell commitment in a permissive manner: FL by inducing proliferation of Ly6D+CD135+CD127+CD19− progenitors and IL-7 by providing survival signals to these progenitors.

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Section: Ly6dmentioning

confidence: 99%

Permissive roles of cytokines interleukin-7 and Flt3 ligand in mouse B-cell lineage commitment

Muenchow

Albertí-Servera

Klein

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…TSLP levels are also increased in the synovial fluid of patients with rheumatoid arthritis, indicating its role in non-allergic inflammation (Koyama et al, 2007). TSLP is expressed in a range of cell types, including epithelial cells, fibroblasts, keratinocytes, airway smooth muscle cells, mast cells and dendritic cells (He and Geha, 2010;Kashyap et al, 2011). In the reproductive organs, TSLP expression in trophoblast cells and decidual epithelial cells and its possible involvement in maternal-fetal tolerance have been reported (Li and Guo, 2009;Guo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Interleukin-1β stimulates the secretion of thymic stromal lymphopoietin (TSLP) from endometrioma stromal cells: possible involvement of TSLP in endometriosis

et al. 2012

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study question: Is thymic stromal lymphopoietin (TSLP) involved in the pathophysiology of endometriosis? summary answer: TSLP is up-regulated by interleukin (IL)-1b and may be involved in the development of endometriosis. what is known already: Endometriosis is a chronic inflammatory disease in which the Th2 immune response is activated and has been suggested to promote the disease. TSLP is a master cytokine that drive Th2 immune response. study design, size, duration: A laboratory study. participants/materials, setting, methods: Primary cultures of endometrioma stromal cells (ESCs) were treated with IL-1b, a typical inflammatory cytokine associated with endometriosis. Gene expression of TSLP in ESCs and secretion of TSLP protein from ESCs were studied using quantitative PCR and a specific ELISA. Interferon g (IFNg), a typical Th1 cytokine, and IL-4, a typical Th2 cytokine, were added to the culture to evaluate their effect on the IL-1b-induced secretion of TSLP. Inhibitors of p38 mitogen-activated protein kinase (MAPK), p42/44 MAPK and stress-activated protein kinase/Jun amino-terminal kinase (SAPK/JNK) were added to the culture to examine intracellular signals involved in IL-1b-induced TSLP secretion. The expression of TSLP in endometrioma tissue was examined by immunohistochemistry. The concentration of TSLP in the serum and peritoneal fluid (PF) of women with or without endometriosis was measured with a specific ELISA.main results and the role of chance: IL-1b stimulated the expression of TSLP mRNA and secretion of TSLP protein from ESCs. IL-4 enhanced the IL-1b-induced TSLP secretion from ESCs, while IFNg reduced it. Inhibitors of p42/44 MAPK, p38 MAPK and SAPK/JNK suppressed the IL-1b-induced secretion of TSLP from ESCs. Positive immunostaining of TSLP was observed in the stroma of endometrioma tissue. TSLP concentrations in the serum and PF were both higher in women with endometriosis compared with those without endometriosis.limitations, reasons for caution: The present study was only in vitro. The samples used for culture were endometrioma tissues, not including other types of endometriosis. Therefore, the present findings should be interpreted with caution.wider implications of the findings: This study provided new insights in the Th2 immune response-related mechanism in endometriosis.

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“…Consistent with this viewpoint, TSLP was originally reported to exert its T H 2-promoting properties through dendritic cell-mediated pathways in human beings that involved the induction of the OX40 ligand on dendritic cells [28]. TSLP has been implicated in the development of asthma and atopic dermatitis [13]. In atopic diseases such as asthma and atopic dermatitis, not only dendritic cells, epithelial cells, eosinophils, and T cells but also mast cells are important.…”

Section: Introductionmentioning

confidence: 73%

Down-regulation of thymic stromal lymphopoietin by curcumin

Moon

Jeong

Kim

2013

Pharmacological Reports

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Abstract:Background: Thymic stromal lymphopoietin (TSLP) is a cytokine implicated in the pathogenesis of allergic diseases such as asthma, atopic dermatitis and allergic rhinitis. Curcumin has various effects such as antidepressant, antioxidant, antihyperglycemic, antitumor and anti-inflammatory. However, the effect of curcumin on the production of TSLP has not been clarified. Thus, we investigated how curcumin inhibits the expression and production of TSLP in the human mast cell line, HMC-1 cells. Methods: We used enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and caspase-1 assay to investigate the effects of curcumin. Results:The results show that curcumin inhibited the production and mRNA expression of TSLP in HMC-1 cells: the maximal inhibition rate of TSLP production by curcumin (50 µM) was 59.16 ± 4.20%. In addition, curcumin suppressed the nuclear factor-kB luciferase activity induced by phorbol myristate acetate plus A23187. In the activated HMC-1 cells, caspase-1 activity was increased, whereas caspase-1 activity was decreased by pretreatment with curcumin. Conclusion: These results suggest that curcumin can be used to treat inflammatory and atopic diseases through the suppression of TSLP.

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Thymic Stromal Lymphopoietin Is Produced by Dendritic Cells

Cited by 128 publications

References 37 publications

Permissive roles of cytokines interleukin-7 and Flt3 ligand in mouse B-cell lineage commitment

Permissive roles of cytokines interleukin-7 and Flt3 ligand in mouse B-cell lineage commitment

Interleukin-1β stimulates the secretion of thymic stromal lymphopoietin (TSLP) from endometrioma stromal cells: possible involvement of TSLP in endometriosis

Down-regulation of thymic stromal lymphopoietin by curcumin

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