Thymic hormonal effect on human peripheral blood lymphocytes in vitro. III. Conditions for mixed lymphocyte-tumor culture assay

Shoham, Jacob; Eshel, Ilana

doi:10.1016/0022-1759(80)90312-9

Cited by 14 publications

(3 citation statements)

References 16 publications

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“…TS is able to increase some immunological functions in experimental (D'Agostaro et al, 1980;Shoam and Eshe, 1980;Falchetti et al, 1981;Falchetti et al,1982) and clinical (Aiuti et al, 1979;Davies and Levinsky, 1982;Lin et al, 1987;Touraine and Sanhadjik, 1992) studies.…”

Section: T Is Well Established That the Bursa Of Fabriciusmentioning

confidence: 99%

Identification of cells secreting a thymostimulin-like substance and examination of some histoenzymatic pathways in aging avian primary lymphatic organs: II. Bursa of Fabricius

Mazzone¹,

Aita²,

Gabrielli³

et al. 2009

Eur J Histochem

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The Bursa of Fabricius of 15 day, 1-, 3-, and 6 month-old adult chickens (White Leghorn strain) were studied by histological and histochemical staining, histoenzymatic reactions (LDH, SDH, α-GPDH, NAD, NADPH, Ca ++ -dependent ATP-ase, pH 8.5) and by anti-thymostimulin immunoreaction. Positive reactions for mucopolysaccharides and enzymatic activities were located in the epithelia of the follicles, i.e. in follicleassociated-epithelium (FAE), inter-follicle-epithelium (IFE) and in different epithelial compartments of cortical and medullary zones. Positive reaction for thymostimulin-like (TSlike) substance was restricted to FAE cells and weakly to the basal lamina of IFE. In 6-month-old chickens, the FAE cells disappeared; the phenomenon of bursal regression was evident, although not all the follicles were involved. In the few still normal follicles, the good reactivity to the enzymes tested suggests that residual physiological activity is still present, even if reduced. , 1621) is a primary lymphoid organ peculiar to birds. The bursa is responsible for the maturation and differentiation of B-lymphocytes to produce humoral immunological responses. The other primary lymphoid organ, the thymus, instead, is responsible for the maturation and differentiation of T-lymphocytes, leading to cellular immunological responses (Dent and Good, 1965;Schaffner et al., 1974a;Le Douarin et al., 1984).The structure of the bursa has been described both during the ontogenetic period (Jolly, 1911a(Jolly, , 1914Cordier, 1969;Edwards et al., 1975;Le Douarin et al., 1975;Le Douarin et al., 1976;Le Douarin et al., 1984;Ritter and Lebacq, 1977) and during the posthatching period up to its involution (Jolly, 1911b(Jolly, ,1914Mc Connachie and Ruth, 1973;Glick, 1974Glick, , 1983Bellamy and Mohamed, 1982;Davenport and Allen, 1985).Since the discovery that the bursa was implicated in the differentiation of B-lymphocytes for humoral immunity (Chang et al., 1955;Glick et al., 1956;Papermaster and Good, 1962;Peterson and Good, 1965), attention was focused, on the one hand, on lymphoid cell populations (Ackermann and Knouff, 1959;Ackermann, 1962;Woods and Linna, 1965;Cooper et al., 1966; Toivanen et al., 1972 a,b;Toivanen et al., 1974;Back et al., 1973;Grossi et al., 1974;Grossi et al., 1976;Glick, 1974Glick, , 1983Glick, , 1995Glick et al., 1975;Eerola et al., 1982;Brand et al., 1983;Pink et al., 1985;Lassila, 1989;Nowak et al., 1990;Motyka and Reynolds, 1991;Petrini et al., 1991;Paramithiotis and Ratcliffe, 1993, 1994 a,b, 1996Reynaud and Weill, 1993;Masteller and Thompson, 1994;Masteller et al., 1995;Paramithiotis et al., 1995;Obranovich and Boyd, 1996;Funk and Thompson, 1996) and, on the other hand, on the epithelial stroma providing the bursal microenvironment within which B-cell maturation and differentiation occurs.

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Section: T Is Well Established That the Bursa Of Fabriciusmentioning

confidence: 99%

Identification of cells secreting a thymostimulin-like substance and examination of some histoenzymatic pathways in aging avian primary lymphatic organs: II. Bursa of Fabricius

Mazzone¹,

Aita²,

Gabrielli³

et al. 2009

Eur J Histochem

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“…In comparison to T cells, the role of thymic hormones in controlling tumor progression is understudied. Thymic peptide hormones, including thymosins, thymulin and thymopoietin, are secreted by TEC and can either act locally on thymocytes or circulate in the periphery to impact the differentiation and function of T cells and other immune subsets ( 1 , 7 , 8 ). Purified peptides from the thymus as well as thymic peptide analogues and cytokines have been trialled in several studies investigating their impact on the response and tolerability of standard chemotherapy, radiotherapy, or both ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

Thymus-derived hormonal and cellular control of cancer

Savino

Lepletier

2023

Front. Endocrinol.

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The thymus gland is a central lymphoid organ in which developing T cell precursors, known as thymocytes, undergo differentiation into distinct type of mature T cells, ultimately migrating to the periphery where they exert specialized effector functions and orchestrate the immune responses against tumor cells, pathogens and self-antigens. The mechanisms supporting intrathymic T cell differentiation are pleiotropically regulated by thymic peptide hormones and cytokines produced by stromal cells in the thymic microenvironment and developing thymocytes. Interestingly, in the same way as T cells, thymic hormones (herein exemplified by thymosin, thymulin and thymopoietin), can circulate to impact immune cells and other cellular components in the periphery. Evidence on how thymic function influences tumor cell biology and response of patients with cancer to therapies remains unsatisfactory, although there has been some improvement in the knowledge provided by recent studies. Herein, we summarize research progression in the field of thymus-mediated immunoendocrine control of cancer, providing insights into how manipulation of the thymic microenvironment can influence treatment outcomes, including clinical responses and adverse effects of therapies. We review data obtained from clinical and preclinical cancer research to evidence the complexity of immunoendocrine interactions underpinning anti-tumor immunity.

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“…For the in vitro assay, P815 lymphoblast-like murine mastocytoma cells were used, a cell line commonly used to study immunological reactions in various tumor models (17,18). The mixed lymphocyte-tumor culture is a widely used technique, which has been implemented before for the analysis of tumor cell behavior (19)(20)(21)(22)(23)(24). Also, the assessment of tumor cell vitality by flow cytometry is a commonly used method (25)(26)(27)(28).…”

mentioning

confidence: 99%

Flow cytometric analysis of the graft‐versus‐Leukemia‐Effect After Hematopoietic Stem Cell Transplantation in Mice

et al. 2015

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Acute Graft-versus-Host-Disease (aGvHD) is one of the major complications following allogeneic hematopoietic stem cell transplantation (HSCT). Although rather helpful, the use of conventional immunosuppressive drugs leads to general immunosuppression and is toxic. The effects of CD41 T-cells, in respect to the development of aGvHD, can be altered by administration of antihuman CD4 monoclonal antibodies, here MAX.16H5 IgG 1 . This approach must be tested for possible interference with the Graft-versus-Leukemia-Effect (GvL). Thus, in vitro experiments were conducted, exposing P815 leukemic cells to bone marrow and splenocytes from cd4

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Thymic hormonal effect on human peripheral blood lymphocytes in vitro. III. Conditions for mixed lymphocyte-tumor culture assay

Cited by 14 publications

References 16 publications

Identification of cells secreting a thymostimulin-like substance and examination of some histoenzymatic pathways in aging avian primary lymphatic organs: II. Bursa of Fabricius

Identification of cells secreting a thymostimulin-like substance and examination of some histoenzymatic pathways in aging avian primary lymphatic organs: II. Bursa of Fabricius

Thymus-derived hormonal and cellular control of cancer

Flow cytometric analysis of the graft‐versus‐Leukemia‐Effect After Hematopoietic Stem Cell Transplantation in Mice

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