Thymectomy in Newborn and Adult Mice*

Sjodin, Kenneth; Dalmasso, Agustin P.; Smith, James M.; Martínez, Cristina

doi:10.1097/00007890-196301040-00011

Cited by 129 publications

(41 citation statements)

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“…After induction of diabetes some mice underwent thymectomy as described (22). Briefly, a Pasteur pipette was introduced into the mediastinum through a sternotomy and the thymus was removed by suction.…”

Section: Thymectomymentioning

confidence: 99%

Antigen Exposure during Enhanced CTLA-4 Expression Promotes Allograft Tolerance In Vivo

Salvalaggio¹,

Camirand

Ariyan³

et al. 2006

The Journal of Immunology

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The role of CTLA-4 in tolerance is primarily inferred from knockout and blocking studies. Anti-CD45RB mediates allograft tolerance in mice by inducing CTLA-4 expression on CD4 cells, providing a novel opportunity to determine how therapeutic enhancement of CTLA-4 promotes tolerance. We now show that induced CTLA-4 expression normally resolves by day 17. Although thymectomy prolongs enhanced CTLA-4 expression, long-term engraftment is unaffected. To address the temporal relationship between increased CTLA-4 expression and engraftment, transplantation was delayed for various times after anti-CD45RB treatment. Delaying transplantation for 7 days (when CTLA-4 expression had peaked but treatment mAb was no longer detectable), resulted in long-term engraftment comparable to transplantation with no delay (day 0). Delaying transplantation from 10 to 18 days led to a progressively poorer outcome as CTLA-4 expression returned to baseline. This suggested that Ag exposure while CTLA-4 expression is enhanced is sufficient to induce long-term engraftment. To substantiate this, on day 0, anti-CD45RB-treated mice received BALB/c vs unrelated alloantigen, followed by transplantation of BALB/c islets 10 days later. Whereas recipients exposed to unrelated Ag experienced acute rejection, recipients exposed to donor Ag achieved long-term engraftment. Anti-CD45RB-treated mice exposed to alloantigen exhibited anergic CD4+CD25− effector cells and regulatory CD4+CD25+ cells. Moreover, CD25 depletion in the peritransplant period prevented anti-CD45RB-mediated engraftment. Thus, exposure of CD4 cells expressing CTLA-4 to donor Ag is necessary and sufficient to induce long-term engraftment which appears to be mediated by both regulation and anergy.

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Section: Thymectomymentioning

confidence: 99%

Antigen Exposure during Enhanced CTLA-4 Expression Promotes Allograft Tolerance In Vivo

Salvalaggio¹,

Camirand

Ariyan³

et al. 2006

The Journal of Immunology

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“…Mice thymectomized and sham-thymectomized at 4 wk were ordered from Taconic Farms (Germantown, NY) or were thymectomized in-house (13). Mice were tail bled at various days post-thymectomy and stained with directly conjugated mAbs.…”

Section: Thymectomymentioning

confidence: 99%

Murine CD8+ Recent Thymic Emigrants are αE Integrin-Positive and CC Chemokine Ligand 25 Responsive

Staton

Johnston

Butcher

et al. 2004

The Journal of Immunology

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Recent thymic emigrants (RTE) are an important subpopulation of naive CD8+ T cells because of their ability to reconstitute a diverse immune system after periods of T cell depletion. In neonatal mice, the majority of peripheral T lymphocytes are RTE, cells that have recently left the thymus to populate the periphery. Postulating that these cells could have unique trafficking mechanisms, we compared adhesion molecule and chemokine receptor expression of neonatal RTE with mature adult lymphocytes. Neonatal CD8+ splenocytes uniformly express αE integrin and exhibit a high responsiveness to CC chemokine ligand (CCL25) (as compared with adult CD8+ splenocytes). Mature CD8+ thymocytes have a similar αE integrin+ CCL25 responsive phenotype, as do adult CD8+ RTE identified by intrathymic FITC injection. With increasing age, the frequency of CD8+ αE integrin+ splenocytes decreases, roughly correlating with thymic involution. Moreover, halting thymic output by thymectomy accelerates the age-dependent decline in peripheral CD8+ αE integrin+ RTE phenotype cells. Low expression of CD44 distinguishes these CD8+ RTE from a population of memory phenotype αE integrin+ CD8+ cells that are CD44high. We conclude that CD8+ RTE have unique adhesive and chemotactic properties that distinguish them from naive CD8+ T cells. These properties may enable specialized microenvironmental and cell-cell interactions contributing to the fate of RTE in the periphery during the early post-thymic period. This phenotype will also facilitate the identification and isolation of RTE for further studies.

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“…The mice were thymectomized using the method described by Sjodin et al (1963). Thymectomy was performed on 14-dayold animals since in our hands thymectomy at this age resulted in fewer mortalities due to technique than thymectomy of fully mature animals.…”

Section: Imrnmunosuppressionmentioning

confidence: 99%

Transplantation of Primary Explants of Human Tumour to Mice Treated with Antilymphocyte Serum

Phillips¹,

Gazet²

1970

Br J Cancer

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Thymectomy in Newborn and Adult Mice*

Cited by 129 publications

References 0 publications

Antigen Exposure during Enhanced CTLA-4 Expression Promotes Allograft Tolerance In Vivo

Antigen Exposure during Enhanced CTLA-4 Expression Promotes Allograft Tolerance In Vivo

Murine CD8+ Recent Thymic Emigrants are αE Integrin-Positive and CC Chemokine Ligand 25 Responsive

Transplantation of Primary Explants of Human Tumour to Mice Treated with Antilymphocyte Serum

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