Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.)

Jardim-Messeder, Douglas; Caverzan, Andréia; Rauber, Rafael; Cunha, Juliana R.; Carvalho, Fernanda Torres de; Gaeta, Marcos Letaif; Fonseca, Guilherme Cordenonsi da; Costa, J.M.; Frei, Michael; Silveira, Joaquim Albenísio Gomes da; Margis, Rogério; Saibo, Nelson J. M.; Margis‐Pinheiro, Márcia

doi:10.1016/j.envexpbot.2018.02.012

Cited by 21 publications

(25 citation statements)

References 48 publications

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“…To understand the mechanical properties in response to high altitude, the stomatal size and stomatal density in leaf epidermis was measured. The pollutant ozone and pathogenic microbes can result in stomatal closure and thereby prevent damaging entry to the leave [15–17]. As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

The adaptation strategies of Herpetospermum pedunculosum (Ser.) Baill at altitude gradient of the Tibetan plateau by physiological and metabolomic methods

Zhao

Liu

et al. 2019

BMC Genomics

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Background Herpetospermum pedunculosum (Ser.) Baill is annual scandent herbs. They are used in the treatment of piles, inflammation of the stomach and the intestines. It can survive the extreme environment of the Tibetan Plateau (TP). However, the underlying mechanisms of this adaptation to H . pedunculosum from TP remain unclear. Here, we combined physiological and metabolomics methods to analyze H . pedunculosum response to altitude gradient differences. Results At high altitude, increases in the activities of Ascorbate peroxidase (APX), Glutathione reductase (GR), Dehydroascorbate reductase (DHAR), Monodehydroascorbate reductase (MDHAR), Superoxide dismutase (SOD) have been observed in leaves. Total Glutathion content, total Ascorbate content and the ASA (ascorbic acid)/docosahexaenoic acid (DHA) ration were highly elevated from low altitude to high altitude. In addition, high altitude induces decrease of the Anthocyanidin content (ANTH) and increase of abscisic acid content (ABA). The GC-MS analyses identified of 50 metabolites from leaves of H. pedunculosum . In addition, a metabolic network was constructed based on metabolomic datasets using a weighted correlation network analysis (WGCNA) approach. The network analysis uncovered 4 distinguished metabolic modules highly associated with I, II, III and IV respectively. Furthermore, the analysis successfully classified 50 samples into seven groups: carbohydrates, amino acids, organic acids, lipid components, polyamine, secondary metabolism and others. Conclusions In the present study, the content of parts of amino acid components increased in samples collected at higher altitudes, and most of metabolites, including carbohydrates and organic acids were assigned to the carbon metabolic pathway comprising reductive pentose phosphate pathway, glycolysis and TCA cycle, indicating the direct relationship between adaptability and the carbon metabolic pathway and amino acids in H. pedunculosum response to high altitude. The results of this study laid the foundation of the molecular mechanism on H . pedunculosum from high altitude. Electronic supplementary material The online version of this article (10.1186/s12864-019-5778-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

The adaptation strategies of Herpetospermum pedunculosum (Ser.) Baill at altitude gradient of the Tibetan plateau by physiological and metabolomic methods

Zhao

Liu

et al. 2019

BMC Genomics

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“…The best-described mechanism is that mediated by abscisic acid that recruits calcium ions, nitric oxide (NO), H 2 O 2 and regulatory phosphorylation [ 193 , 194 ]. Guard cells generate H 2 O 2 by means of amine oxidases [ 195 ], peroxidases and RBOHs [ 196 , 197 ]. The activity of RBOHs is regulated by Ca 2+ binding [ 198 ] and phosphorylation by protein kinase OST1 (OPEN STOMATA 1) [ 199 ], which in turn is regulated by abscisic acid and interacts with a peroxiporin [ 200 , 201 ].…”

Section: H 2 O 2 In Growth Amentioning

confidence: 99%

Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks

Černý

Habánová

Berka

et al. 2018

IJMS

164

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Hydrogen peroxide (H2O2) is steadily gaining more attention in the field of molecular biology research. It is a major REDOX (reduction–oxidation reaction) metabolite and at high concentrations induces oxidative damage to biomolecules, which can culminate in cell death. However, at concentrations in the low nanomolar range, H2O2 acts as a signalling molecule and in many aspects, resembles phytohormones. Though its signalling network in plants is much less well characterized than are those of its counterparts in yeast or mammals, accumulating evidence indicates that the role of H2O2-mediated signalling in plant cells is possibly even more indispensable. In this review, we summarize hydrogen peroxide metabolism in plants, the sources and sinks of this compound and its transport via peroxiporins. We outline H2O2 perception, its direct and indirect effects and known targets in the transcriptional machinery. We focus on the role of H2O2 in plant growth and development and discuss the crosstalk between it and phytohormones. In addition to a literature review, we performed a meta-analysis of available transcriptomics data which provided further evidence for crosstalk between H2O2 and light, nutrient signalling, temperature stress, drought stress and hormonal pathways.

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“…These responses indicate that drought treatment was able to induce stronger changes in the proteomic profile in NT plants as compared to apx8 silenced ones. A possible explanation for these interesting results consists in the fact that changes induced by APX8 silencing in rice plants could be able to produce a pre-acclimation condition to abiotic stress [18]. Accordingly, the initial changes in apx8 plants should result in a reduced plasticity of proteomic profile when a second stress situation was imposed, which was already observed in other studies involving apx gene family silencing in rice [11][12][13].…”

Section: Silenced Plants Accumulate H 2 O 2 In Both Control and Mild mentioning

confidence: 56%

“…Unfortunately, in the majority of the carried out works, the plants are exposed to acute water deficit, a condition that is commonly associated to drastic stress symptoms [17]. Recently, we demonstrated that rice plants deficient in thylakoidal APX exposed to an acute drought stress triggered increased H 2 O 2 accumulation which was associated with stomatal closing, evidencing the role of that enzyme in these processes [18].…”

Section: Introductionmentioning

confidence: 88%

“…CO 2 assimilation is initially decreased by stomatal limitation and further by biochemical restriction in the Calvin-Benson cycle reactions, especially by decrease in Rubisco activity [43]. Previously, we have demonstrated that apx8 plants display high stomatal restriction associated to H 2 O 2 accumulation in response to acute water deficit [18]. In this study, data suggest that higher reduction in photosynthetic CO 2 assimilation in silenced plants is due to restriction in both stomatal and Rubisco activity, assuming that Vcmax is a good indicator for activity of this enzyme, as has been widely reported [44].…”

Section: Silenced Plants Accumulate H 2 O 2 In Both Control and Mild mentioning

confidence: 98%

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Proteomic and physiological approaches reveal new insights for uncover the role of rice thylakoidal APX in response to drought stress

Cunha

Carvalho

Neto

et al. 2019

Journal of Proteomics

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This work provides for the first time an integrative study involving proteomic, physiological and biochemical analyses directed to elucidation of thylakoidal APX roles for drought tolerance in rice plants. Our data reveal that this enzyme is crucial for maintaining of growth and photosynthesis under mild water deficit conditions. This essential role is related to maintaining of HO homeostasis and accumulation of essential proteins involved in several important metabolic pathways. Remarkably, for drought resistance was essential the accumulation of proteins involved with metabolism of photosynthesis, signaling, carbohydrates, protein synthesis/degradation and stress. These results can contribute to understand the role of chloroplast ascorbate peroxidases in drought tolerance, highlighting the physiological importance of key proteins in this process.

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Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.)

Cited by 21 publications

References 48 publications

The adaptation strategies of Herpetospermum pedunculosum (Ser.) Baill at altitude gradient of the Tibetan plateau by physiological and metabolomic methods

The adaptation strategies of Herpetospermum pedunculosum (Ser.) Baill at altitude gradient of the Tibetan plateau by physiological and metabolomic methods

Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks

Proteomic and physiological approaches reveal new insights for uncover the role of rice thylakoidal APX in response to drought stress

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