Thy-1: More than a Mouse Pan-T Cell Marker

Haeryfar, S. M. Mansour; Hoskin, David W.

doi:10.4049/jimmunol.173.6.3581

Cited by 156 publications

(159 citation statements)

References 81 publications

Supporting

Mentioning

152

Contrasting

Unclassified

Order By: Relevance

“…Thy1.1 is expressed on T cells in the mouse, but its expression is variable between species (Haeryfar and Hoskin, 2004). The level of Thy1.1 staining seemed low in samples from some ferrets, indicating that, as in the mouse, there may be allelic forms of this molecule (Johnson andPorter, 2000, Yan, et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

Rutigliano

Doherty

Franks

et al. 2008

Journal of Immunological Methods

View full text Add to dashboard Cite

Influenza virus infections carry a high public health cost, and pandemics are potentially catastrophic. Though the ferret is generally regarded as the best model for human influenza, no reagents are available for the analysis of cellular immunity. We thus screened monoclonal antibodies (mAbs) made for identifying immune cells in other species to see if any were cross-reactive. Flow cytometric analysis of lymphocytes isolated from blood, spleen, and lung of normal and virus-infected ferrets indicated that several mouse mAbs bound to the corresponding antigens in ferrets. Typing bronchoalveolar lavage populations from pneumonic ferrets with mAb to human CD8 showed the massive CD8+ T cell enrichment characteristic of this infection in mice. The availability of this, and several other mAbs that showed cross-reactivity, should allow us to begin the dissection of cellmediated immunity in the ferret, which, at least from these early results, looks similar to the situation in mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

Rutigliano

Doherty

Franks

et al. 2008

Journal of Immunological Methods

View full text Add to dashboard Cite

show abstract

“…CD90 or THY-1 was originally discovered as a thymocyte antigen (19,20) but further reports have shown expression of this protein in human fibroblasts, neurons, endothelial cells, as well as in murine T-cells (21,22). This protein is involved in T-cell activation (23) and has widespread nonimmunologic functions, including inhibition of neurite outgrowth (24), apoptotic signaling (25), leukocyte and melanoma cell adhesion and migration (26,27), tumor suppression (28,29), and fibroblast proliferation and migration (30)(31)(32).…”

Section: Discussionmentioning

confidence: 99%

Differential expression of CD90 and CD14 stem cell markers in malignant breast cancer cell lines

et al. 2012

View full text Add to dashboard Cite

The recently emerged concept of cancer stem cell (CSC) has led to a new hypothesis on the basis for tumor progression. Basically, the CSC theory hypothesizes the presence of a hierarchically organized and relatively rare cell population, which is responsible for tumor initiation, self-renewal, and maintenance, in addition to accumulation of mutation and resistance to chemotherapy. CSCs have recently been described in breast cancer. Different genetic markers have been used to isolate breast CSCs, none of which have been correlated with the tumorigenicity or metastatic potential of the cells, limiting their precise characterization and clinical application in the development of therapeutic protocols. Here, we sought for subpopulations of CSCs by analyzing 10 judiciously chosen stem cell markers in a normal breast cell line (MCF10-A) and in four human breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-435, and Hs578-T) displaying different degrees of metastatic and invasiveness potential. We were able to identify two markers, which are differentially expressed in nontumorigenic versus tumor cells. The CD90 marker was highly expressed in the malignant cell lines. Interestingly, the CD14 molecule displayed higher expression levels in the nontumorigenic cell line. Therefore, we demonstrated that these two markers, which are more commonly used to isolate and characterize stem cells, are differentially expressed in breast tumor cells, when compared with nontumorigenic breast cells. ' 2012 International Society for Advancement of Cytometry Key terms CD90; Thy-1; breast cancer; cancer stem cell; CD14; breast cancer stem cell

show abstract

“…But in contrast to humans, mouse Thy-1 is present on the surface of mouse thymocytes and peripheral T cells. The physiological ligand or interacting molecule for mouse Thy-1 in the lymphoid compartment has not been identified (33).…”

Section: Discussionmentioning

confidence: 99%

Thy-1 (CD90) Is an Interacting Partner for CD97 on Activated Endothelial Cells

Wandel

Saalbach

Sittig

et al. 2012

The Journal of Immunology

View full text Add to dashboard Cite

Leukocyte recruitment in response to inflammatory signals is governed, in part, by binding to Thy-1 (CD90) on activated endothelial cells (EC). In this study, we characterized the adhesion G-protein coupled receptor CD97, present on peripheral myeloid cells, as a novel interacting partner for Thy-1. CD97 was upregulated on polymorphonuclear cells (PMNC) of patients with psoriasis. In psoriatic skin lesions, CD97+ myeloid cells colocalized with Thy-1+ EC of small vessels in microabscesses, suggesting an interaction between CD97 and Thy-1 that was further examined by adhesion and protein-binding assays. PMNC and cell lines stably overexpressing CD97 adhered specifically to Thy-1+–activated human dermal EC, Thy-1+ CHO cells, and immobilized Thy-1 protein. Binding of the CD97+ CHO clones correlated with their CD97 expression level. Soluble CD97 bound specifically to immobilized Thy-1 protein, as well as Thy-1+–activated EC and CHO cells. In all assays, cellular adhesion or protein binding was blocked partially by CD97 and Thy-1–blocking mAb. Our data suggested that CD97 interacts via its stalk with Thy-1 because mAb directed to the stalk of CD97 showed stronger blocking compared with mAb to its epidermal growth factor-like domains, and binding was calcium independent. Moreover, soluble CD97 without the stalk and soluble EMR2, containing highly homologous epidermal growth factor-like domains but a different stalk, failed to bind. In summary, binding of leukocytes to activated endothelium mediated by the interaction of CD97 with Thy-1 is involved in firm adhesion of PMNC during inflammation and may play a role in the regulation of leukocyte trafficking to inflammatory sites.

show abstract

Thy-1: More than a Mouse Pan-T Cell Marker

Cited by 156 publications

References 81 publications

Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

Differential expression of CD90 and CD14 stem cell markers in malignant breast cancer cell lines

Thy-1 (CD90) Is an Interacting Partner for CD97 on Activated Endothelial Cells

Contact Info

Product

Resources

About