Thy-1 knockdown retards wound repair in mouse skin

Lee, Min Jung; Shin, Jeong Oh; Jung, Han Sung

doi:10.1016/j.jdermsci.2012.11.009

Cited by 21 publications

(24 citation statements)

References 35 publications

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“…In addition, increased proliferation of fibroblasts in healing wounds of Thy-1-deficient mice compared with wild-type mice confirmed the importance of Thy-1 for the control of proliferation in vivo. In accordance, Lee et al (2013) described increased proliferation rates in Thy-1 siRNA-treated NIH3T3 cells.…”

Section: Discussionsupporting

confidence: 72%

Controlling the Balance of Fibroblast Proliferation and Differentiation: Impact of Thy-1

Schmidt

Gutknecht

Simon

et al. 2015

Journal of Investigative Dermatology

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Fibroblasts provide matrix and regulatory mediators to the microenvironment and thereby contribute to maintenance of tissue homeostasis, wound healing, and tumor progression. In the present study, we addressed the functional relevance of Thy-1 for fibroblast functions in vitro and in vivo. Using fibroblasts from Thy-1(-/-) and wild-type mice, recombinant expression of Thy-1, and analysis of the interaction of fibroblasts with immobilized Thy-1, we showed that Thy-1 has a crucial role in the control of cell growth by suppressing proliferation and promoting apoptosis and differentiation of dermal fibroblasts. Function-blocking studies revealed that Thy-1 mediates the control of apoptosis and proliferation via modulation of β3 integrin function. Interestingly, Thy-1-mediated growth control appears to be a more general mechanism because it also regulates growth of tumor cells of different origin dependent on their β3 integrin expression. In summary, our findings point to an important role of Thy-1 in controlling the balance between proliferation and differentiation in dermal fibroblasts.

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Section: Discussionsupporting

confidence: 72%

Controlling the Balance of Fibroblast Proliferation and Differentiation: Impact of Thy-1

Schmidt

Gutknecht

Simon

et al. 2015

Journal of Investigative Dermatology

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“…Accordingly, only Thy-1-positive human myometrial and orbital fibroblasts were able to differentiate into myofibroblasts after TGF-b stimulation (45). Consistently, Thy-1-deficient mice displayed diminished granulation tissue formation, increased fibroblast proliferation, decreased apoptosis, and down-regulation of FasL (37,46,47). Generally, Thy-1 plays significant roles in tissue repair and fibrosis.…”

Section: Fibroblastsmentioning

confidence: 87%

Thy‐1: more than a marker for mesenchymal stromal cells

Saalbach

Anderegg

2019

FASEB j.

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Balanced differentiation of mesenchymal stromal cells (MSCs) into osteoblasts and adipocytes is essential for healthy bone and fat homeostasis. A disturbed balance of MSC differentiation results in bone loss and increased adipogenesis as observed in several human conditions, such as osteoporosis, obesity, and aging. This reflects the importance of MSC fate decision. Therefore, it is important to identify factors that regulate the balance between osteogenic and adipogenic differentiation of MSCs so as to identify new targets to improve bone formation in osteoporosis and control adipose tissue development. There is accumulating evidence that thymus cell antigen 1 (Thy‐1), also known as CD90, expressed on MSCs, plays a critical role in the fate decision of MSCs. Recently, Thy‐1 has been shown to promote osteogenic differentiation of MSCs and thus bone formation while inhibiting adipogenic differentiation and restricting adipose tissue accumulation. The clinical importance of these findings was validated by the detection of reduced serum soluble Thy‐1 in patients with disturbed bone remodeling. In this review, we aim to summarize data on the impact of Thy‐1 on the control of MSC differentiation and its consequences for bone and fat formation.—Saalbach, A., Anderegg, U. Thy‐1: more than a marker for mesenchymal stromal cells. FASEB J. 33, 6689–6696 (2019). http://www.fasebj.org

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“…78 CD90 has been linked to efficient wound closure. 79 A more detailed comprehension of these molecular functions should improve the understanding of both physiological and pathological skin remodeling and repair.…”

Section: Discussionmentioning

confidence: 99%

Altered Dermal Fibroblasts in Systemic Sclerosis Display Podoplanin and CD90

Nazari

Rice

Stifano

et al. 2016

The American Journal of Pathology

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Tissue injury triggers the activation and differentiation of multiple cell types to minimize damage and initiate repair processes. In systemic sclerosis, these repair processes appear to run unchecked, leading to aberrant remodeling and fibrosis of the skin and multiple internal organs, yet the fundamental pathological defect remains unknown. We describe herein a transition wherein the abundant CD34 þ dermal fibroblasts present in healthy human skin disappear in the skin of systemic sclerosis patients, and CD34 À , podoplanin þ , and CD90 þ fibroblasts appear. This transition is limited to the upper dermis in several inflammatory skin diseases, yet in systemic sclerosis, it can occur in all regions of the dermis. In vitro, primary dermal fibroblasts readily express podoplanin in response to the inflammatory stimuli tumor necrosis factor and IL-1b. Furthermore, we show that on acute skin injury in both human and murine settings, this transition occurs quickly, consistent with a response to inflammatory signaling. Transitioned fibroblasts partially resemble the cells that form the reticular networks in organized lymphoid tissues, potentially linking two areas of fibroblast research. These results allow for the visualization and quantification of a basic stage of fibroblast differentiation in inflammatory and fibrotic diseases in the skin. (Am J Pathol 2016, 186: 2650e2664; http://dx

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Thy-1 knockdown retards wound repair in mouse skin

Cited by 21 publications

References 35 publications

Controlling the Balance of Fibroblast Proliferation and Differentiation: Impact of Thy-1

Controlling the Balance of Fibroblast Proliferation and Differentiation: Impact of Thy-1

Thy‐1: more than a marker for mesenchymal stromal cells

Altered Dermal Fibroblasts in Systemic Sclerosis Display Podoplanin and CD90

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