Thy-1 expression, a possible marker of early myofibroblast development, in renal tubulointerstitial fibrosis induced in rats by cisplatin

Immunohistochemical characterization of glial fibrillary acidic protein (GFAP)-expressing cells in a rat liver cirrhosis model induced by repeated injections of thioacetamide (TAA)

“…Thy-1 is a glycophosphatidylinositol (GPI)-linked outer membrane leaflet glycoprotein expressed by mesenchymal stem cells in the bone marrow and pericytes (Bae et al, 2008;Yuasa et al, 2013), and has been reported to correlate with myofibroblastic differentiation from the stromal stem cells (Dezso et al, 2007;Rege and Hagood, 2006). In hepatic fibrosis, Thy-1-positive cells in the Glisson's sheath may migrate into injured areas as a precursor of myofibroblasts (Dezso et al, 2007).…”

Participation of bone morphogenetic protein (BMP)-6 and osteopontin in cisplatin (CDDP)-induced rat renal fibrosis

“…CDDP injection to rats could induce renal epithelial damage and subsequent interstitial fibrosis as reported previously (Yamamoto et al, 2012;Yuasa et al, 2013). The consistently increased levels of BUN and creatinine were indicative of persistent renal failure by CDDP.…”

Calponin Expression in Renal Tubulointerstitial Fibrosis Induced in Rats by Cisplatin

“…Previously, we showed that myofibroblasts in cisplatin (CDDP)-induced rat renal interstitial fibrosis could exhibit varying degrees of expression of vimentin, desmin and α-SMA 13 . To shed some light on the pathogenesis of renal tubulointerstitial fibrosis, in this study, we investigated calponin expression in correlation with vimentin, desmin or α-SMA expressions by using immunolabeling.…”

“…To shed some light on the pathogenesis of renal tubulointerstitial fibrosis, in this study, we investigated calponin expression in correlation with vimentin, desmin or α-SMA expressions by using immunolabeling. In addition, we carried out comparisons of calponin and Thy-1 expressions, because Thy-1, a GPI-anchored cell surface protein, is regarded as a possible marker of early myofibroblast development 13 .…”

“…Forty-two 6-week-old male F344/DuCrj rats (Charles River Laboratories Japan, Hino, Shiga, Japan) were injected intraperitoneally with a single dose (6 mg/kg body weight) of CDDP (Nippon Kayaku Co. Ltd., Tokyo, Japan) 13 . They were housed in an animal room controlled at 22 ± 3°C with a 12 hour light-dark cycle and allowed free access to a standard commercial diet (MF, Oriental Yeast Co. Ltd., Tokyo, Japan) and tap water.…”