Thunbergia laurifolia leaf extract partially recovers lead-induced renotoxicity through modulating the cell signaling pathways

Rana, Mohammad Nasiruddin; Karim, Naymul; Changlek, Suksan; Ma, Rahman; Tangpong, Jitbanjong; Hajjar, Dina; Alelwani, Walla; Makki, Arwa A.

doi:10.1016/j.sjbs.2020.08.016

Cited by 10 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pathogenesis and pathophysiology of Pb-induced toxicity have been linked to the production of free radicals that generate oxidative stress and deplete the antioxidant defense system (Nasiruddin et al, 2020;Gadde et al, 2021). According to our research, the birds in the Pb-induced toxicity group showed a noticeably higher level of hydrogen peroxide (H2O2) production, lipid peroxidation product (MDA) content, and oxidative stress in the liver of the experimental birds.…”

Section: Discussionmentioning

confidence: 78%

Immunohistochemical and morphological changes associated with hepatic damage in lead acetate-induced toxicity and mitigatory properties of naringin in cockerel chicks

Esan,

Ajibade,

Ebirim

et al. 2023

NJPS

View full text Add to dashboard Cite

Lead (Pb) toxicity constitutes a major health hazard to both humans and animals especially in the developing countries. It is a ubiquitous environmental contaminant found in the air essentially because of unregulated mining and other industrial activities. Lead can be found naturally in the soil thus, contaminating crops for human and animal food, as well as run-off water and air pollution. Intensively and extensively reared domestic chickens are exposed to contamination via inhalation and ingestion of contaminated food materials. Naringin, a product from citrus plant has been described to possess excellent metal chelating ability. Naringin is rich in flavonoid with attendant antioxidant, anti-autophagy, anti-inflammatory, hepatoprotective and cardio-nephroprotective properties. This study was conducted to investigate the hepatoprotective and modulation of oxidative stress in commercial cockerel chickens by Naringin. Thirty-six commercial cockerel chickens were randomly assigned into six groups A-F of six birds each viz: Group A served as control group while groups B, C, and D received Lead acetate at 300 ppm via drinking water continuously till the end of the experiment. In addition, groups C and D were treated with Naringin at 80 mg/kg and 160mg/kg, respectively, via oral gavage for 8 weeks. Groups E and F were administered naringin only at 80mg/kg and 160mg/kg respectively for eight weeks. Pb toxicity induced degenerative changes in the histological sections as well as, higher expression of hepatic caspase 3 as shown by immunohistochemistry. There was increased oxidative stress markers (H2O2, MDA) and depletion of the antioxidant defense system markers SOD, GPx, GSH, and GST. It concluded that Co- treatment with Naringin ameliorated oxidative stress, enhanced antioxidant defense system, reduced the expression of hepatic caspase 3 thus, offering protection against lead acetate-induced derangements in the liver of commercial cockerel chickens

show abstract

Section: Discussionmentioning

confidence: 78%

Immunohistochemical and morphological changes associated with hepatic damage in lead acetate-induced toxicity and mitigatory properties of naringin in cockerel chicks

Esan,

Ajibade,

Ebirim

et al. 2023

NJPS

View full text Add to dashboard Cite

show abstract

“…Our ndings also con rmed earlier reports on toxicity of NaF on cardiovascular system (Oyagbemi et We observed from our study that NaF intoxication caused signi cant increase in blood urea nitrogen (BUN) and creatinine levels. The increase in BUN and creatinine has been associated with various degrees of renal injuries (Nasiruddin et al 2020;Ni et al 2021). The observed nephrotoxicity by NaF might be due to free radical generation and increase protein catabolism with concomitant systemic oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

L- Arginine Supplementation Protections Sodium Fluoride-Induced Nephrotoxicity And Hypertension By Suppressing Mineralocorticoid Receptor and Angiotensin Converting Enzyme Activity

Oyagbemi

Awodele

Ajibade

et al. 2021

Preprint

View full text Add to dashboard Cite

Sodium fluoride (NaF) is one of the neglected environmental toxicants that has continued to silently cause toxicity to both humans and animals. NaF is universally present in water, soil and the atmosphere. The persistent and alarming rate of increase in cardiovascular and renal diseases and disorders caused by chemicals such as sodium fluoride (NaF) in mammalian tissues have led to the use of various drugs for the treatment of these diseases. This study aims at evaluating the renoprotective and antihypertensive effects of L- Arginine on NaF-induced nephrotoxicity. Thirty male Wistar rats (150 – 180 g) were used in this study. The rats were randomly divided into five groups of six rats each as Control, NaF (300 ppm), NaF + L- Arginine (100 mg/kg), NaF + L- Arginine (200 mg/kg), and NaF + Lisinopril (10 mg/kg), respectively; orally for eight days. Histopathological examination and immunohistochemistry of renal angiotensin converting enzyme (ACE) and mineralocorticoid receptor (MCR) were performed. Markers of renal damage, oxidative stress, antioxidant defence system, and blood pressure parameters were determined. L- Arginine significantly (p <0.05) ameliorated the hypertensive effects of NaF. The systolic, diastolic and mean arterial blood pressure of the treated groups were significantly (p< 0.05) reduced compared with the hypertensive group. This finding was concurrent with significantly increased serum bioavailability of nitric oxide in the hypertensive treated groups. Also, there was significant reduction in the level of blood urea nitrogen (BUN) and creatinine in the serum of the hypertensive rats treated with L- arginine. There was significant (p<0.05) reduction in markers of oxidative stress such as hydrogen peroxide (H2O2), malondialdehyde (MDA) and protein carbonyl (PCO) and concurrent increase in the levels of antioxidant enzymes in the kidney of hypertensive rats treated with L arginine. The results of this study suggest that L- Arginine normalized high blood pressure, reduced oxidative stress, reduced the expression of renal ACE and MCR, and improved nitric oxide production. Thus, L- Arginine holds promise as a potential therapy against hypertension and renal damage.

show abstract

“…could mitigate cadmium (Cd)-induced organ damage in the liver and kidney, blood and urine Cd levels remained unchanged. Similarly, Rana et al [16] reported partial recovery of Cd-damaged kidney organs in rats treated with RC water crude extract. Notably, RC treatment (100 and 200 mg/kg body weight) led to a reduction in the expression of TNF-α, iNOS, and COX-2, all of which are inflammatory cytokines [16].…”

Section: Introductionmentioning

confidence: 87%

Identification of Pheophytin a and Hydroxy Pheophytin a from Rang Chuet (Thunbergia laurifolia Linn.) as Potent NQO-1 Inducers in Liver Cells

Moeurng,

Posridee,

Kamkaew

et al. 2024

Foods

View full text Add to dashboard Cite

Thunbergia laurifolia Linn. (Rang Chuet, RC), a Thai medicinal plant, possesses various bioactive compounds with potential health benefits. This study aimed to identify detoxifying compounds within RC crude extract. RC leaves were extracted using the Soxhlet method with chloroform. Total carotenoids, chlorophylls, extract yield, total phenolic contents (TPCs), and total flavonoid contents (TFCs) were measured. The extract’s composition was analyzed. Cytotoxicity and effects on the detoxification enzyme NQO-1 were assessed in liver cell lines (AML12 and HepG2) using MTT and NQO-1 assays, respectively. Bioactive fractions were identified using fractionation techniques and mass spectrometry (LC-MS). RC extract displayed significant levels of carotenoids (0.375 mg/g), chlorophylls (2.682 mg/g), and favorable yield (15.3%). TPC and TFC were 363.776 mg/g and 112.22 mg/g of extract, respectively. Analysis revealed phenolic acids (gallic acid, caffeic acid), flavonoid (apigenin), chlorophylls (chlorophylls a, b, pheophytin a and b), and lutein. Among the fractions, Fraction 3 (F3) exhibited the highest NQO-1 enzyme activity. F3 contained pheophytin a and hydroxy pheophytin a, confirmed by LC-MS (m/z 871.59+ [M + H]+ and 887.59+ [M + H]+). F3 significantly induced NQO-1 activity in both HepG2 (3.908-fold) and AML12 (1.99-fold) cells. This study identified F3 from RC extract as a promising fraction containing pheophytin a and hydroxy pheophytin a, responsible for inducing the detoxification enzyme NQO-1 in liver cells. These findings suggest RC’s potential for promoting detoxification.

show abstract

Thunbergia laurifolia leaf extract partially recovers lead-induced renotoxicity through modulating the cell signaling pathways

Abstract: Graphical abstract

Cited by 10 publications

References 40 publications

Immunohistochemical and morphological changes associated with hepatic damage in lead acetate-induced toxicity and mitigatory properties of naringin in cockerel chicks

Immunohistochemical and morphological changes associated with hepatic damage in lead acetate-induced toxicity and mitigatory properties of naringin in cockerel chicks

L- Arginine Supplementation Protections Sodium Fluoride-Induced Nephrotoxicity And Hypertension By Suppressing Mineralocorticoid Receptor and Angiotensin Converting Enzyme Activity

Identification of Pheophytin a and Hydroxy Pheophytin a from Rang Chuet (Thunbergia laurifolia Linn.) as Potent NQO-1 Inducers in Liver Cells

Contact Info

Product

Resources

About