Despite advances in instruments, suture materials, and techniques, thrombosis at the anastomotic site is still a problem in microvascular anastomosis. Various pharmacologic agents have been used to inhibit or suppress the process of thrombosis, and the results have been variable.In the present study, a scanning electron microscopic was used to evaluate the roles of aspirin, dipyridamole, and prostaglandin D2 (PGD2) to assess their effects in microvascular anastomosis.
Materials and MethodsThirty-five New Zealand rabbits weighing between 2 and 4 kg were selected and divided into a control group and three experimental groups. The animals were anesthetized with intramuscular ketamine hydrochloride (30 mg/kg) and intravenous pentobarbital (20 mg/kg). Supplemental doses were added to maintain a good level of surgical anesthesia.The groins of the animals were shaved and painted with povidone iodine and draped sterile. A 4-cm-long incision was made in the femoral region to expose the femoral vessels. Proximal and distal microclips were applied and a longitudinal arteriotomy was then made in the femoral artery. The arteriotomy incision was closed using 10 &dquo;0&dquo; BV-6 monofilament nylon (Ethicon, Inc., Sommerville, NJ). A Zeiss operating microscope and standard microinstruments were used.Following closure of the arteriotomy, blood was permitted to flow for 15 minutes. The microclips were then applied 5 mm below and above the arteriotomy site, and the segment of the artery between the clips was excised under the microscope (25x), opened longitudinally, and gently washed with normal saline solution. The flattened artery was fixed with a buffered 2% glutaraldehyde (pH 7.2) solution for 5 minutes, then stored in the same solution for 24 hours. The specimen was dehydrated by serial passage through 30%, 50%, 75%, and 95% ethanol.