Thrombus organization and healing in the swine experimental aneurysm model. Part I. A histological and molecular analysis

Lee, Daniel; Yuki, Ichiro; Murayama, Yuicm; Chiang, Alexander; Nishimura, Ichiro; Vinters, Harry V.; Wang, Chiachien J.; Nien, Yih-Lin; Ishil, Akira; Wu, Benjamin M.; Viñuela, Fernando

doi:10.3171/jns-07/07/0094

Cited by 45 publications

(44 citation statements)

References 54 publications

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“…9,13,35,54,55 Early inflammatory changes 39,54,55 were followed by a centripetal myofibroblast infiltration, robust collagen deposition, and a thick neointima, which completely sealed the neck. 48 When all specimens showed permanent occlusions and favorable healing characteristics at some delayed time points, as seen in swine aneurysms, then some earlier timeframe was chosen, in hopes of detecting accelerated healing with some new material.…”

Section: Pathologic Analyses Of Treated Aneurysmsmentioning

confidence: 99%

In Vivo Experimental Intracranial Aneurysm Models: A Systematic Review

Bouzeghrane

Naggara

Kallmes³

et al. 2009

AJNR Am J Neuroradiol

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SUMMARY: Animal models are necessary to develop and test innovations in aneurysm therapy before clinical introduction. This review aims at identifying the most likely candidates for standardizing preclinical testing of aneurysm devices. We systematically searched electronic databases for publications on animal aneurysm models from 1961-2008 to assess the methodologic quality of the studies and collect data on the patency and angiographic and pathologic outcomes of treatments. There has been a steady increase in the annual number of publications with time. Species that were most frequently used were dogs, rabbits, and rodents, followed by swine. Most publications are single-laboratory studies with variables and poorly validated outcome measures, a small number of subjects, and limited standardization of techniques. The most appropriate models to test for recurrences after endovascular occlusion were the surgical bifurcation model in dogs, and the elastase-induced aneurysm model in rabbits. A standardized multicenter study is needed to improve the preclinical evaluation of endovascular devices in aneurysm therapy.

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Section: Pathologic Analyses Of Treated Aneurysmsmentioning

confidence: 99%

In Vivo Experimental Intracranial Aneurysm Models: A Systematic Review

Bouzeghrane

Naggara

Kallmes³

et al. 2009

AJNR Am J Neuroradiol

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“…Results of previous studies already demonstrated the paramount importance of aneurysm wall smooth muscle cells in thrombus organization and neointima formation. 6,19 Therefore, it can be hypothesized that loss of mural cells is causative for the failure of luminal thrombus transformation into stable fibrotic tissue.…”

Section: Luminal Thrombus Formationmentioning

confidence: 99%

Loss of Mural Cells Leads to Wall Degeneration, Aneurysm Growth, and Eventual Rupture in a Rat Aneurysm Model

Marbacher

Marjamaa

Bradacova

et al. 2014

Stroke

105

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Background and Purpose— The biological mechanisms predisposing intracranial saccular aneurysms to growth and rupture are not yet fully understood. Mural cell loss is a histological hallmark of ruptured cerebral aneurysms. It remains unclear whether mural cell loss predisposes to aneurysm growth and eventual rupture. Methods— Sodium dodecyl sulfate decellularized and nondecellularized saccular aneurysm from syngeneic thoracic aortas were transplanted to the abdominal aorta of Wistar rats. Aneurysm patency and growth was followed up for 1 month with contrast-enhanced serial magnetic resonance angiographies. Endoscopy and histology of the aneurysms were used to assess the role of periadventitial environment, aneurysm wall, and thrombus remodeling. Results— Nondecellularized aneurysms (n=12) showed a linear course of thrombosis and remained stable. Decellularized aneurysms (n=12) exhibited a heterogeneous pattern of thrombosis, thrombus recanalization, and growth. Three of the growing aneurysms (n=5) ruptured during the observation period. Growing and ruptured aneurysms demonstrated marked adventitial fibrosis and inflammation, complete wall disruption, and increased neutrophil accumulation in unorganized intraluminal thrombus. Conclusions— In the presented experimental setting, complete loss of mural cells acts as a driving force for aneurysm growth and rupture. The findings suggest that aneurysms missing mural cells are incapable to organize a luminal thrombus, leading to recanalization, increased inflammatory reaction, severe wall degeneration, and eventual rupture.

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“…11,19,25 They are not currently considered appropriate for this purpose because of their propensity for thrombosis and complete closure of aneurysmal necks with thick neointima formation in all animals at 1 month, no matter what material is used. Reports of experimental aneurysms in other species (eg, canines and rabbits) have demonstrated a much greater incidence of long-term patency and a lower incidence of spontaneous hemorrhage (essentially 0) than were observed in our study.…”

Section: Aspect Ratio and Giant Aneurysm Modelmentioning

confidence: 99%

“…Our findings suggest that thrombosis is accompanied by degeneration of the aneurysm wall, as found in other experimental or human aortic aneurysms. [9][10][11][12] Inflammation of the venous wall was present in unruptured and in ruptured cases. In our model, the distinguishing factor was the presence of recanalization between the thrombus and the necrosing wall.…”

Section: Thrombosis Followed By Rupturesmentioning

confidence: 99%

“…7,8 Thrombusprecipitating rupture has also been implicated in abdominal aortic aneurysms. [9][10][11][12] However, because thrombosis followed by organization and neointimal closure of the neck is required for successful occlusion of aneurysms by endovascular embolization, 11,[13][14][15][16][17][18] there must be additional factors to account for thrombosis leading to rupture rather than occlusion. Such discriminating factors have not been explored experimentally, to our knowledge.…”

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confidence: 99%

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Thrombosis Heralding Aneurysmal Rupture: An Exploration of Potential Mechanisms in a Novel Giant Swine Aneurysm Model

Raymond¹,

Darsaut

Kotowski³

et al. 2012

AJNR Am J Neuroradiol

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Thrombus organization and healing in the swine experimental aneurysm model. Part I. A histological and molecular analysis

Abstract: This investigation provides a detailed histological and molecular analysis of thrombus organization in the swine aneurysm model. The companion study will describe the effect of embolic bioabsorbable polymers on this process.

Cited by 45 publications

References 54 publications

In Vivo Experimental Intracranial Aneurysm Models: A Systematic Review

In Vivo Experimental Intracranial Aneurysm Models: A Systematic Review

Loss of Mural Cells Leads to Wall Degeneration, Aneurysm Growth, and Eventual Rupture in a Rat Aneurysm Model

Thrombosis Heralding Aneurysmal Rupture: An Exploration of Potential Mechanisms in a Novel Giant Swine Aneurysm Model

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