Thrombus Formation with Rehydrated, Lyophilized Platelets

Fischer, Thomas; Merricks, Elizabeth P.; Bode, Patrick A.; Bellinger, Dwight A.; Russell, Karen E.; Reddick, Robert L.; Sanders, William E.; Nichols, Timothy C.; Read, Marjorie S.

doi:10.1080/1024533021000047954

Cited by 29 publications

(34 citation statements)

References 46 publications

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“…Earlier failures were attributed to inadequate preservation of platelet integrity during processing. Later attempts to address this issue utilized novel means of cross‐linking and additives that demonstrated improved stability, but resulted in significant loss of function . More recent LHP products have shown promise primarily through the use of novel stabilizers such as paraformaldehyde and trehalose .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a lyophilized platelet‐derived hemostatic product

et al. 2019

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BACKGROUND Current limitations of platelet shelf life to 5 days have led to an increasingly greater demand for hemostatic agents with greater longevity. The objective of this study was to evaluate the function of a lyophilized platelet‐derived hemostatic product (thrombosome [TS]) as a potential alternative to fresh platelets. METHODS Platelets were collected from whole blood from healthy donors. TSs were reconstituted with water and added to various configurations of reassembled whole blood (platelets, plasma, and RBCs); measures included rotational thromboelastometry (ROTEM), optical aggregometry, mitochondrial function, calibrated automated thrombogram, collagen adhesion under flow (shear flow assay), and flow cytometry. RESULTS In ROTEM, no differences were observed between maximum clot formation values for contact pathway activation thromboelastometry tests with TSs or platelet samples. Significantly decreased aggregation was observed in the TSs versus platelets (p < 0.001 for all agonists). Flow cytometry measures demonstrated significant decreases in glycoprotein Ib expression and increases in phosphatidylserine expression in the TS group (p < 0.01). The calibrated automated thrombogram assay was suggestive (lag time and peak thrombin) that the TSs might have some thrombogenic properties. Measurements of mitochondrial function revealed that TSs had no functional mitochondria. CONCLUSION In this study, TSs were shown to have nonfunctional mitochondria. ROTEM measures revealed that the TSs had no impact on clot strength. Likewise, compared to platelets, the TSs displayed minimal aggregation, had significantly more phosphatidylserine (measure of activation status), but had the ability to adhere to a collagen surface under flow conditions and contribute to clot formation and induced greater thrombin generation.

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Section: Discussionmentioning

confidence: 99%

Evaluation of a lyophilized platelet‐derived hemostatic product

et al. 2019

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“…The density of the integrin α IIb β 3 complex was measured with 125 I‐labeled MoAb 10E‐5, at a concentration of 20 µg per mL (determined to be above the stoichiometric saturating value 17 ). 125 I‐fibrinogen binding to fresh and RL PLTs was compared as detailed elsewhere 10 …”

Section: Methodsmentioning

confidence: 99%

“…First, RL PLTs are activateable ; this is a basic key element for the safety of all hemostatic systems. The incorporation of RL PLTs into forming fibrin gels results in an irreversible activation response that involves shape change, pseudopodia extension, granule centralization and secretion, and cytoskeletal rearrangements 10 . Exposure of RL PLTs to thrombin has been shown to activate multiple intracellular signaling pathways, including protein kinase C and myosin light chain kinase systems.…”

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confidence: 99%

Primary and secondary hemostatic functionalities of rehydrated, lyophilized platelets

et al. 2006

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“…However, despite the optimism displayed in some articles [1] and in several major publications describing the preservation of metabolic activity following rehydration of lyophilised erythrocytes [2] and platelets [3], the use of freeze dried blood is still far from clinical application. Furthermore, there are no reports on the lyophilisation of embryonic or somatic cells, spermatozoa being the only nucleated cells that have been successfully stored in a dehydrated state [4].…”

Section: Introductionmentioning

confidence: 99%

Freeze-Dried Somatic Cells Direct Embryonic Development after Nuclear Transfer

et al. 2008

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The natural capacity of simple organisms to survive in a dehydrated state has long been exploited by man, with lyophylization the method of choice for the long term storage of bacterial and yeast cells. More recently, attempts have been made to apply this procedure to the long term storage of blood cells. However, despite significant progress, practical application in a clinical setting is still some way off. Conversely, to date there are no reports of attempts to lyophilize nucleated somatic cells for possible downstream applications. Here we demonstrate that lyophilised somatic cells stored for 3 years at room temperature are able to direct embryonic development following injection into enucleated oocytes. These remarkable results demonstrate that alternative systems for the long-term storage of cell lines are now possible, and open unprecedented opportunities in the fields of biomedicine and for conservation strategies.

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Thrombus Formation with Rehydrated, Lyophilized Platelets

Cited by 29 publications

References 46 publications

Evaluation of a lyophilized platelet‐derived hemostatic product

Evaluation of a lyophilized platelet‐derived hemostatic product

Primary and secondary hemostatic functionalities of rehydrated, lyophilized platelets

Freeze-Dried Somatic Cells Direct Embryonic Development after Nuclear Transfer

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