Thrombosis and nephritic syndrome in a dog with visceral leishmaniasis

Font, Artur; Closa, Josep Maria; Molina, Ariel Columbié; Mascort, Joan

doi:10.1111/j.1748-5827.1993.tb03907.x

Cited by 23 publications

(29 citation statements)

References 7 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Histopathological findings in the three analyzed dogs (Animals #2, #8 and #20) were in accordance with those described by various authors in cases of leishmaniasis [8,15,23,26,31,36], and corresponded to glomerulonephritis and interstitial nephritis (Fig. 5).…”

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

et al. 2003

View full text Add to dashboard Cite

-Canine leishmaniasis is an endemic disease in the Mediterranean area caused by the protozoan Leishmania infantum, which usually produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot using antibodies to IgG and IgA from dogs were carried out in the urine of 22 dogs with leishmaniasis diagnosed by ELISA and confirmed by PCR, and 20 healthy dogs. The results were compared to renal function laboratory tests and to those from a histopathological study of the kidneys from sick animals that died naturally or were euthanized. Five different bands with molecular weights ranging from 10 to 110 kDa were obtained from the electrophoresis of the urine of healthy dogs. 33.5% of total proteins corresponded to low molecular weight proteins and the other proteins had middle and high molecular weights. However, in the group with leishmaniasis, a maximum of 11 different bands with molecular weights ranging from 10 kDa to 150 kDa were displayed in the electrophoresis of the urine. The urine electrophoretic pattern in the sick dogs was classified as mixed (proteins with high and low molecular weights) because low molecular weight proteins made up 57.9% and the rest of the proteins had middle and high molecular weights. In Western blot, none of the healthy dogs showed excretion of IgG and/or IgA, whereas IgG and IgA were detected in the Western blot of urine of 68% and 55% respectively of dogs with leishmaniasis. The results obtained in the leishmaniasis group agreed with glomerular and tubular damage, which were confirmed by the histopathological findings. leishmaniasis / proteinuria / dog / SDS-PAGE / Western blot

show abstract

Section: Discussionsupporting

confidence: 88%

“…The immune complexes cause a secondary inflammatory reaction [10] and the reduction in the perfusion of the peritubular capillaries leads to tubular and interstitial tissue ischemia [35]. Proteinuria is observed as a result, which can range from moderate to severe according to the evolution of the disease [8].…”

Section: Introductionmentioning

confidence: 99%

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Histological examination (amastigote detection, 18%) and immunohistochemistry (immunolabeling of amastigotes, 40.9%) indicate that the renal lesions cannot be due to Leishmania infection alone. Previous reports suggested that immune complexes might be deposited on the glomeruli capillary wall and blood vessels in cases of glomerulonephritis and vasculitis, because of the persistent production of circulating antigens that activate the complement cascade, which elicits an inflammatory response (Brito et al 1975;Sartori et al 1987;Tafuri et al 1989;Poli et al 1991;Nieto et al 1992;Font et al 1993Font et al , 2004. Considering the controversy between histological changes and parasite density in the lungs and intestines of the present cases, it is possible that the main reason for chronic lesions of these tissues could be associated with immune-complex reactions other than those occurring because of parasite infection (Duarte and Corbett 1984;Duarte et al 1986;Ferrer et al 1991;Gonçalves et al 2003).…”

Section: Discussionmentioning

confidence: 97%

An immunohistochemical study in cases with usual and unusual clinicopathological findings of canine visceral leishmaniosis

Toplu

Aydoğan

2011

Parasitol Res

View full text Add to dashboard Cite

The present study describes pathologic findings and immunohistochemical diagnosis of canine visceral leishmaniasis (CVL) in 22 dogs who died naturally in the Aegean region of Turkey. At necropsy, lymphadenomegaly, hepatosplenomegaly, hepatic, and nephrosclerotic lesions were conspicuous. Histopathologically, chronic inflammatory reactions of the spleen, lymph nodes, bone marrow, liver, and skin were marked findings. Cytological and histological examinations showed macrophages loaded with Leishmania amastigotes in these organs. Immunohistochemistry revealed that immunolabeling of amastigotes and/or parasite antigen, especially in the lymph nodes, spleen, bone marrow, liver, and skin, and occasionally, in the kidneys, intestines and lungs. Our laboratory results showed that immunohistochemistry should be included, along with cytological and histological examinations, in the diagnosis of CVL.

show abstract

“…These aspects differ from animal to animal, organ to organ and animals with and without manifestation of the disease 62 . It is known that CVL causes a wide spectrum of nonspecific clinical signs that may be confused with other infectious diseases, complicating the diagnosis 2,33 . The major clinical manifestations found in symptomatic dogs from endemic areas of Teresina are alopecia, onychogryposis, skin lesions, conjunctivitis, lymphadenopathy and weight loss 5,49,72 .…”

Section: Clinical and Pathological Aspects Of Visceral Leishmaniasismentioning

confidence: 99%

Forty years of visceral leishmaniasis in the State of Piaui: a review

Drumond

Costa²

2011

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYVisceral leishmaniasis (VL) has been known to occur in the state of Piauí since 1934. The typically rural disease began to appear in urban areas over time, being concentrated mainly in Teresina, the capital of Piauí. Teresina was also affected by the first urban epidemic of VL in Brazil. Over 1,000 cases of the disease were reported during urbanization (1981)(1982)(1983)(1984)(1985)(1986)). Human population growth and migration led to land occupation on the outskirts of Teresina. These factors have contributed to vector proliferation, increasing the incidence of VL. At present, the incidence of human and canine disease is quite high and uncontrolled in Piauí. It seems that some measures, such as the elimination of seropositive dogs, failed to significantly reduce the number of new VL cases in Teresina. Despite previously conducted studies, little is known about VL epidemiology in urban areas. The aim of this review is to reveal the situation of VL in Teresina during the last 40 years, focusing on the major factors that may contribute to the high incidence and persistence of VL infection.

show abstract

Thrombosis and nephritic syndrome in a dog with visceral leishmaniasis

Cited by 23 publications

References 7 publications

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

An immunohistochemical study in cases with usual and unusual clinicopathological findings of canine visceral leishmaniosis

Forty years of visceral leishmaniasis in the State of Piaui: a review

Contact Info

Product

Resources

About