Thromboresistant Silicon Plates Modified With Chitosan and Heparin by the Layer-by-Layer Assembly Method

Дрозд, Н. Н.; Shagdarova, Balzhima; Zhuikova, Yulia V.; Ильина, А. В.; Vasiliev, Mikhail; Vasilieva, Tatiana; Hein, Aung Miat; Варламов, В. П.

doi:10.15259/pcacd.24.001

Cited by 5 publications

(3 citation statements)

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“…Addition of ADP/collagen to human plasma led to activation of purinergic receptors P2Y12/glycoprotein receptors GPVI and platelet aggregation [10]. We have previously shown that oligochitosan Ch10/85 2-fold inhibited platelet aggregation induced by ADP or collagen in concentrations of 0.685 or 1.82 mg/ml, respectively [7].…”

Section: Resultsmentioning

confidence: 95%

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Effect of Oligochitosan on Experimental Venous Thrombosis in Guinea Pigs

et al. 2021

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Oligochitosan Сh10/85 with a molecular weight of 10 kDa and a deacetylation degree of 85% prevented the development of experimental venous thrombosis in guinea pigs after intravenous administration in a dose of 30 mg/kg. In a concentration of 0.005-0.5 mg/ml, oligochitosan Ch10/85 did not provoke hemolysis of human red blood cells in in vitro experiments. The antithrombotic effect of oligochitosan Ch10/85 that exhibits low anticoagulant activity (by two orders of magnitude lower than that of unfractionated heparin) can be associated with inhibition of platelet aggregation.

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Section: Resultsmentioning

confidence: 95%

“…Previous studies showed that oligochitosan Ch10/85 had insignificant anticoagulant activity (AA) in the APTT test (about 0.1 U/mg), did not independently contribute to human platelet aggregation, and reduced platelet aggregation induced by ADP or collagen [7].…”

Section: Resultsmentioning

confidence: 99%

Effect of Oligochitosan on Experimental Venous Thrombosis in Guinea Pigs

et al. 2021

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“…The silicone was activated with cold oxygen plasma. Modification of silicone led to an increase in its resistance to the formation of blood clots [ 29 ]. Modification of isocyanate-activated PU by the LbL method using chitosan and UFH also led to an increase in the clotting time and improved the thromboresistance of the material [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

New N-Methylimidazole-Functionalized Chitosan Derivatives: Hemocompatibility and Antibacterial Properties

et al. 2023

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Novel imidazole derivatives of the low molecular weight chitosan N-(2-hydroxypropyl)-1H-1,2,3-triazol-4-yl)methyl)-1-methyl-1H-imidazol-3-ium chitosan chloride (NMIC) were synthesized using copper-catalyzed azide–alkyne cycloaddition (CuAAC). The degrees of substitution (DSs) for the new derivatives were 18–76%. All chitosan derivatives (2000 µg/mL) were completely soluble in water. The antimicrobial activity of the new compounds against E. coli and S. epidermidis was studied. The effect of chitosan derivatives on blood and its components was studied. NMIC samples (DS 34–76%) at a concentration <10 μg/mL had no effect on blood and plasma coagulation. Chitosan derivatives (DS 18–76%) at concentrations of ≥83 μg/mL in blood and ≥116.3 μg/mL in plasma resulted in a prolongation of the clotting time of blood and plasma, positively related to the DS. At concentrations up to 9.1 μg/mL, NMIC did not independently provoke platelet aggregation. The degree of erythrocyte hemolysis upon contact with NMIC samples (2.5–2500 μg/mL) was below 4%. The inhibition of blood/plasma coagulation indicates the promising use of the studied samples to modify the surface of medical materials in order to achieve thromboresistance.

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Plasma surface functionalization: A comprehensive review of advances in the quest for bioinstructive materials and interfaces

Zhianmanesh

Gilmour

Bilek

et al. 2023

Applied Physics Reviews

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Surface biofunctionalization aims to create cell-instructive surfaces that control the behavior of cells and modulate cellular interactions by incorporating cell signaling moieties at the materials–biosystem interface. Despite advances in developing bioinert and biocompatible materials, blood clotting, inflammation, and cell death continue to be observed upon the contact of foreign materials with living tissues leading to the materials' rejection. Specific examples include the application of foreign materials in implantable devices (e.g., bone implants, antimicrobial surfaces, and cardiovascular stents), biosensors, drug delivery, and 3D-bioprinting. Biofunctionalization of materials to date has been predominantly realized using wet chemical approaches. However, the complexity of wet chemistry, toxicity of reactants, waste disposal issues, reaction time, poor reproducibility, and scalability drive a need for a paradigm shift from wet chemical approaches to dry methods of surface biofunctionalization. Plasma-based technologies that enable covalent surface immobilization of biomolecules have emerged as dry, reagent-free, and single-step alternatives for surface biofunctionalization. This review commences by highlighting the need for bioinstructive surfaces and coatings for various biomedical applications such as bone implants, antimicrobial surfaces, biosensors, and 3D-bioprinted structures, followed by a brief review of wet chemical approaches for developing biofunctionalized surfaces and biomimetic devices. We then provide a comprehensive review of the development of plasma-based technologies for biofunctionalization, highlighting the plasma–surface interactions and underpinning mechanisms of biomolecule immobilization.

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Thromboresistant Silicon Plates Modified With Chitosan and Heparin by the Layer-by-Layer Assembly Method

Cited by 5 publications

References 0 publications

Effect of Oligochitosan on Experimental Venous Thrombosis in Guinea Pigs

Effect of Oligochitosan on Experimental Venous Thrombosis in Guinea Pigs

New N-Methylimidazole-Functionalized Chitosan Derivatives: Hemocompatibility and Antibacterial Properties

Plasma surface functionalization: A comprehensive review of advances in the quest for bioinstructive materials and interfaces

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