Thrombopoietin, flt3, and kit ligands together suppress apoptosis of human mobilized CD34+ cells and recruit primitive CD34+Thy-1+ cells into rapid division

Murray, Lesley J.; Young, Judy; Osborne, Linda J.; Luens, Karin M.; Scollay, Roland; Hill, Beth

doi:10.1016/s0301-472x(99)00031-4

Cited by 120 publications

(91 citation statements)

References 34 publications

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“…CD90, also known as Thy-1, is expressed on hematopoietic cells (Imbert et al, 1998;Murray et al, 1999a;Stewart et al, 2000), thymic nurse cells (Bodey et al, 1999), neuronal tissue (Barlow and Huntley, 2000), renal mesangial cells (Tamura et al, 2000), placenta (Bukovsky et al, 1999) and some connective tissues (Saalbach et al, 1999). Human peripheral blood cells positive for both CD90 and CD34 include hematopoietic stem cells capable of producing multiple hematopoietic lineages (Murray et al, 1999a,b;Stewart et al, 2000).…”

Section: Discussion Positive Staining For CD Markers In Human Mesenchmentioning

confidence: 99%

Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors

et al. 2001

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This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity of the stem cells from each population. All populations contained lineage-committed myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as lineageuncommitted pluripotent stem cells capable of forming muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells. Flow cytometric analysis of adult stem cell populations revealed positive staining for CD34 and CD90 and negative staining for CD3, CD4, CD8, CD11c, CD33, CD36, CD38, CD45, CD117, Glycophorin-A, and HLA DR-II. Anat Rec 264: [51][52][53][54][55][56][57][58][59][60][61][62] 2001.

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Section: Discussion Positive Staining For CD Markers In Human Mesenchmentioning

confidence: 99%

Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors

et al. 2001

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“…For example, IL-3, IL-6, stem cell factor (SCF), 22,23 Flt3 ligand, 24 and thrombopoietin (TPO) [25][26][27][28] have been shown to stimulate primitive hematopoietic cells to proliferate ex vivo when present at the optimal concentrations and combinations. Tyrosine phosphorylation of STAT5 in megakaryocytes and platelets after TPO treatment has been well characterized.…”

Section: Introductionmentioning

confidence: 99%

“…Comparable results have also been obtained in cytokine-inducible SH2-containing protein-1 transgenic mice. 11 Somewhat surprisingly, the peripheral hematology of the STAT5ab Ϫ/Ϫ mice was not dramatically altered, although reductions in the numbers of IL-3-and GM-CSF-responsive myeloid progenitors 12 and IL-7-responsive B-lymphoid 12,13 [25][26][27][28] have been shown to stimulate primitive hematopoietic cells to proliferate ex vivo when present at the optimal concentrations and combinations. Tyrosine phosphorylation of STAT5 in megakaryocytes and platelets after TPO treatment has been well characterized.…”

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confidence: 99%

Reduced lymphomyeloid repopulating activity from adult bone marrow and fetal liver of mice lacking expression of STAT5

Bunting

Bradley

Hawley

et al. 2002

Blood

135

134

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IntroductionThe functional roles of janus kinases (JAKs) and signal transducers and activators of transcription (STATs) have been characterized in both hematopoietic and nonhematopoietic tissues. 1 The JAK/STAT signaling axis allows a diverse set of extracellular signals to result in modification of gene expression patterns in the appropriate target cells. This process is initiated when, on cytokine binding, JAKs come into close proximity and become activated by autophosphorylation on tyrosine residues. Activated JAKs then phosphoryate among many other substrates, the STAT proteins. STATs form homodimers or heterodimers via SH2 domain phosphotyrosine interactions and translocate to the nucleus where they bind to transcriptional elements on DNA. 2,3 Activated STAT proteins regulate their target genes often with tissue specificity. In the hematopoietic system, JAK/STAT signaling has been extensively described in a variety of lymphoid and myeloid cell types. Four mammalian JAKs and 7 STAT proteins have been identified and characterized by generation of knockout mice. 4 These studies have revealed a large diversity in the role of STATs in hematopoiesis with some STATs showing phenotypes primarily in restricted cytokine pathways (eg, interferons, interleukin [IL]-4, IL-12, and IL-13) that are primarily involved in immune responses (STAT1, STAT2, STAT4, and STAT6). In contrast, the complex phenotypes of STAT3-and STAT5-deficient mice demonstrate a broad cytokine activation and function profile for many individual cell lineages.STAT5a and STAT5b are 2 very homologous transcription factors with variability between these 2 proteins being primarily in the transactivation domain. Mice deficient in either STAT5a or STAT5b have been generated and characterized. The STAT5a knockout mouse is characterized by defects in responses to granulocyte-macrophage colony-stimulating factor (GM-CSF) 5 and mammary gland development. 6 The STAT5b knockout mouse is characterized by defects in growth hormone signaling and expression of male-characteristic liver gene expression patterns. 7 To study the effects of STAT5 on hematopoiesis and also to eliminate any compensating function between the 2 STAT isoforms, homozygous mutant mice lacking both STAT5a and STAT5b were generated. These female mice were infertile, but heterozygous mice could breed normally and give viable STAT5ab Ϫ/Ϫ mice, but at a lower than expected frequency because of various phenotypes affecting survival. An important role for STAT5a and STAT5b in T-cell function 8,9 and terminal myeloid differentiation/apoptosis 10 has been shown by using tissues from these STAT5ab Ϫ/Ϫ mice. Comparable results have also been obtained in cytokine-inducible SH2-containing protein-1 transgenic mice. 11 Somewhat surprisingly, the peripheral hematology of the STAT5ab Ϫ/Ϫ mice was not dramatically altered, although reductions in the numbers of IL-3-and GM-CSF-responsive myeloid progenitors 12 and IL-7-responsive B-lymphoid 12,13 [25][26][27][28] have been shown to stimulate primitive hematopoi...

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“…Most ex vivo cultures of human HSPCs use cytokine mixtures in serum-free medium that includes SCF, TPO, IL-3, IL-6 and Flt3-L [4]. The manipulation of some signaling pathways such as Notch and Wnt has also shown effectiveness for ex vivo expansion of HSPCs [5].…”

mentioning

confidence: 99%

CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro

Liu¹,

Zhang²,

Zhang³

et al. 2014

Sci. China Life Sci.

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Due to the low number of collectable stem cells from single umbilical cord blood (UCB) unit, their initial uses were limited to pediatric therapies. Clinical applications of UCB hematopoietic stem and progenitor cells (HSPCs) would become feasible if there were a culture method that can effectively expand HSPCs while maintaining their self-renewal capacity. In recent years, numerous attempts have been made to expand human UCB HSPCs in vitro. In this study, we report that caffeic acid phenethyl ester (CAPE), a small molecule from honeybee extract, can promote in vitro expansion of HSPCs. Treatment with CAPE increased the percentage of HSPCs in cultured mononuclear cells. Importantly, culture of CD34 + HSPCs with CAPE resulted in a significant increase in total colony-forming units and high proliferative potential colony-forming units. Burst-forming unit-erythroid was the mostly affected colony type, which increased more than 3.7-fold in 1 μg mL 1 CAPE treatment group when compared to the controls. CAPE appears to induce HSPC expansion by upregulating the expression of SCF and HIF1-α. Our data suggest that CAPE may become a potent medium supplement for in vitro HSPC expansion.hematopoietic stem and progenitor cells, caffeic acid phenethyl ester, expansion Citation:Liu YM, Zhang BW, Zhang J, Wang SH, Yao HL, He LJ, Chen L, Yue W, Li YH, Pei XT. CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro.

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Thrombopoietin, flt3, and kit ligands together suppress apoptosis of human mobilized CD34+ cells and recruit primitive CD34+Thy-1+ cells into rapid division

Cited by 120 publications

References 34 publications

Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors

Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors

Reduced lymphomyeloid repopulating activity from adult bone marrow and fetal liver of mice lacking expression of STAT5

CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro

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