Thromboplastins, Heparin, and Polybrene

Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales

“…In order to avoid possible neutralization effects of polybrene, added in the majority of commercial reagents a homemade thromboplastin was used. Polybrene is a polycation that can form complexes with polyanions, such as heparin and possibly the sulfated polysaccharide assessed here, blocking their action (Carroll, 1999). Even with the homemade thromboplastin, no effect was observed.…”

Anticoagulant Activity of a Unique Sulfated Pyranosic (1→3)-β-l-Arabinan through Direct Interaction with Thrombin

“…However, when the concentration of the polysaccharide was increased, longer PT were observed (supplemental Table 1). These results were attributed to the presence of Polybrene in the commercial reagent (46). On the other hand, APTT and TT were prolonged in a concentration-dependent manner, and the anticoagulant activity proved to be lower than that of heparin and somewhat higher than that of dermatan sulfate (supplemental Table 1).…”