“…Two alleles were also shown to encode proteins of higher molecular weight than the wild type protein [49,50]. Eight alleles were shown, by the glycosidase-or tunicamycin-mediated restoration of normal molecular weight, to encode proteins carrying an additional N-glycan [27,30,31,33,36,38,45]. Finally, six studies documented the presence of an additional glycan on the corresponding protein by western blotting, glycosidase digestion and HPLC [26,28,29,34,41 ,51], and three others demonstrated the attachment of a glycan by western blotting, glycosidase digestion and site-directed mutagenesis [32,35,37,43,47].…”