Thromboinflammatory effects of RBC microvesicles

Stavrou, Evi X.

doi:10.1182/blood.2020004985

Cited by 4 publications

(4 citation statements)

References 10 publications

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“… 28 , 29 EMVs and PS+EMVs trigger the coagulation process and thrombin generation through several pathways. 24 , 25 , 30 , 31 Our results, consistent with others’, 13 , 25 , 30 , 31 indicated that significant decrements occurred in coagulation time (PT and CT assays) in RBCs throughout the time, without any significant differences among the filters. Moreover, the inverse correlations between EMVs/μL and the assays verified the possible role of EMVs in coagulation activities and thromboembolic complications, as suggested before.…”

Section: Discussionsupporting

confidence: 91%

The potential effect of leukocyte filtration methods on erythrocyte-derived microvesicles: One step forward

Roshanzamir

Amini-Kafiabad²,

Zarif³

et al. 2022

Eur J Transl Myol

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By harmonizing the pre-preparation conditions and also removing some donors’ variations, the current study took one step forward to investigate whether different leukocyte filtration sets influence the quality of RBCs throughout the storage time. Twelve whole blood units were collected, and each unit was split into three equal parts. Thirty-six divided bags were filtered using three different leukocyte-filtration sets including Red Cell and Whole Blood Filters (12 units per filter). The prepared RBCs were refrigerated for up to 42 days and assessed for microvesicle count and size, clotting- and prothrombin time, hemolysis index, and biochemical parameters. A significant increment in erythrocytes microvesicle count (EMVs/μL) was observed during the time in the three filtration sets. The number of EMVs in WBF-RBCs was higher (~1.6 fold) than in F-RCF on day 42 (p=0.035). Interestingly the median fluorescence intensity of EMVs decreased during the storage. The size of MVs rose during the time without any significant differences among the filters. Coagulation time decreased in RBCs over the storage, with no significant differences among the filters. Hemolysis index and lactate concentration increased while glucose level decreased significantly throughout the time. The changes in WBF-RBCs were more drastic rather than RCF-RBCs. The only significant difference in the count of EMVs was between WBF and F-RCF components on day 42. Though the changes in WBF products were more drastic, all the values fell within the standard limits. Accordingly, all three filtration sets can be considered.

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Section: Discussionsupporting

confidence: 91%

The potential effect of leukocyte filtration methods on erythrocyte-derived microvesicles: One step forward

Roshanzamir

Amini-Kafiabad²,

Zarif³

et al. 2022

Eur J Transl Myol

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“…In order to investigate if there is prothrombotic tendency in alloHCT survivors, after adjusting for the existence of CV risk factors, we measured promising markers of thrombosis already investigated in the field of CV disease, which is platelet and erythrocyte MVs [12,43]. Indeed, a link with thrombosis has been established not only for platelet MVs, but also recently for erythrocyte MVs [17,44]. Our study confirms for the first time this link in alloHCT recipients, suggesting that vascular injury and pro-coagulant activity may represent mutually reinforcing and interdependent processes in these patient group, independently of the presence of traditional CV risk factors.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, platelet microvesicles (PMVs) participate in several homeostatic responses and pathogenic processes including inflammation, atherosclerosis, immunity, and cancer [ 16 ]. Recently, erythrocyte MVs have also been implicated in patients with increased CV risk [ 12 ], with emerging evidence from basic research that suggests a potent thrombo-inflammatory role [ 17 ]. Such novel markers also aim to better characterize the substantial residual CV risk in high-risk patients, which is not effectively treated with current therapeutic options [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation

Gavriilaki

Sakellari

Anyfanti

et al. 2020

IJMS

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(1) Background: survivors of allogeneic hematopoietic cell transplantation (alloHCT) suffer from morbidity and mortality due to cardiovascular events. We hypothesized that vascular injury and pro-coagulant activity are evident in alloHCT survivors without existing alloHCT complications or relapse. (2) Methods: we enrolled consecutive adult alloHCT survivors without established cardiovascular disease and control individuals matched for traditional cardiovascular risk factors (January–December 2019). Circulating microvesicles (MVs) of different cellular origins (platelet, erythrocyte, and endothelial) were measured by a standardized flow cytometry protocol as novel markers of vascular injury and pro-coagulant activity. (3) Results: we recruited 45 survivors after a median of 2.3 (range 1.1–13.2) years from alloHCT, and 45 controls. The majority of patients suffered from acute (44%) and/or chronic (66%) graft-versus-host disease (GVHD). Although the two groups were matched for traditional cardiovascular risk factors, alloHCT survivors showed significantly increased platelet and erythrocyte MVs compared to controls. Within alloHCT survivors, erythrocyte MVs were significantly increased in patients with a previous history of thrombotic microangiopathy. Interestingly, endothelial MVs were significantly increased only in alloHCT recipients of a myeloablative conditioning. Furthermore, MVs of different origins showed a positive association with each other. (4) Conclusions: endothelial dysfunction and increased thrombotic risk are evident in alloHCT recipients long after alloHCT, independently of traditional cardiovascular risk factors. An apparent synergism of these pathophysiological processes may be strongly involved in the subsequent establishment of cardiovascular disease.

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“…This finding suggests that RBC EVs support factor XII-dependent thrombin generation [54]. Noubouossie et al revealed RBC EVs activate both factor XII (FXII) and prekallikrein, leading to factor IX (FIX) activation by two independent pathways: the classic FXIIa-FXI-FIX pathway and direct kallikrein activation of FIX [43,55]. Rubin et al also showed that RBC EVs have FXI-dependent procoagulant properties and can initiate TG in plasma in the absence of exogenous tissue factors [6].…”

Section: Procoagulant Activity and Hemostasismentioning

confidence: 99%

Red Blood Cell-Derived Extracellular Vesicles: An Overview of Current Research Progress, Challenges, and Opportunities

Ma,

Xia,

Gong

et al. 2023

Biomedicines

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Red blood cell-derived extracellular vesicles (RBC EVs) are small, spherical fragments released from red blood cells. These vesicles, similar to EVs derived from other cell types, are crucial for intercellular communication processes and have been implicated in various physiological and pathological processes. The diagnostic and therapeutic potential of RBC EVs has garnered increasing attention in recent years, revealing their valuable role in the field of medicine. In this review, we aim to provide a comprehensive analysis of the current research status of RBC EVs. We summarize existing studies and highlight the progress made in understanding the characteristics and functions of RBC EVs, with a particular focus on their biological roles in different diseases. We also discuss their potential utility as diagnostic and prognostic biomarkers in diseases and as vectors for drug delivery. Furthermore, we emphasize the need for further research to achieve selective purification of RBC EVs and unravel their heterogeneity, which will allow for a deeper understanding of their diverse functions and exploration of their potential applications in diagnostics and therapeutics.

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Thromboinflammatory effects of RBC microvesicles

Cited by 4 publications

References 10 publications

The potential effect of leukocyte filtration methods on erythrocyte-derived microvesicles: One step forward

The potential effect of leukocyte filtration methods on erythrocyte-derived microvesicles: One step forward

Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation

Red Blood Cell-Derived Extracellular Vesicles: An Overview of Current Research Progress, Challenges, and Opportunities

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