Thrombocytes respond to lipopolysaccharide through Toll-like receptor-4, and MAP kinase and NF-κB pathways leading to expression of interleukin-6 and cyclooxygenase-2 with production of prostaglandin E2

Scott, T. R.; Owens, Marcy Dimmick

doi:10.1016/j.molimm.2007.07.035

Cited by 89 publications

(66 citation statements)

References 40 publications

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“…They express the major histocompatibility complex II and co-stimulatory molecules CD40, CD80, and CD86 (Ferdous et al, 2008). In addition of being unconventional, these expressions resemble innate effector cells in function and have a unique role in affecting adaptive immunity in chickens (Scott & Owens, 2008). It has been shown that the in-vitro treatment of thrombocytes induces iNOS gene expression (Ferdous, 2014); yet, standard vaccination appears to be insufficient to stimulate chicken thrombocytes to increase its numbers or induce the expression of pro-inflammatory mediators (Ferdous, 2014).…”

Section: Effects Of Glycyrrhizic Acid On Productive and Immune Paramementioning

confidence: 99%

Effects of Glycyrrhizic Acid on Productive and Immune Parameters of Broilers

Ocampo

Gómez-Verduzco

Tapia-Pérez

et al. 2016

Rev. Bras. Cienc. Avic.

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KeywordsBroiler chickens, growth promotion, immune system, glycyrrhizic acid.Submitted: February/2015 Approved: March/2016 ABSTRACT Considering that glycyrrhizic acid (GRA) has been shown to have in-vitro and in-vivo antiviral activity against a wide range of viruses as well as immunostimulating activity, a trial to evaluate its effects on the performance and the immune response against Newcastle disease of broiler chickens was carried out. The study was performed with oneday-old Ross x Ross broiler chickens. GRA was added to the drinking water throughout the 49-d production cycle at a dose of 0.03%. Sample size of the trial was established in a pilot assay. Results showed that broiler chickens treated with GRA presented better weight gain, final body weight, feed conversion ratio, and lower mortality rate than the non-treated controls. In addition, GRA-treated birds presented higher antibody titers against Newcastle disease virus and more efficient cellular immune response, as demonstrated by the late-hypersensitivity response test. Blood lymphocyte and thrombocyte counts also increased in this group. The histopathological examination of the bursa, spleen, and thymus revealed that only the thymus of the GRA-treated group had a clearly defined increase in cortex thickness on day 49. The bursa showed a higher number of lymphoid lesions in CG on days 21 and 49 compared with the GRA group. These results suggest that GRA has growth promotion properties, which are possibly linked to immunebased effects.

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Section: Effects Of Glycyrrhizic Acid On Productive and Immune Paramementioning

confidence: 99%

Effects of Glycyrrhizic Acid on Productive and Immune Parameters of Broilers

Ocampo

Gómez-Verduzco

Tapia-Pérez

et al. 2016

Rev. Bras. Cienc. Avic.

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“…In addition to these residues, murine MD-2 is predicted to contain a third glycosylation site at position 150. Interestingly, chMD-2 does not contain the two conserved Asn 26 and Asn 114 residues. Instead, it has two putative glycosylation sites at the positions 41 and 49, in addition to the Asn-X-Ser/Thr motif around position Asn 150 as in mMD-2.…”

Section: Analysis Of Chicken Md-2mentioning

confidence: 99%

Unique Properties of the Chicken TLR4/MD-2 Complex: Selective Lipopolysaccharide Activation of the MyD88-Dependent Pathway

Keestra

Putten

2008

The Journal of Immunology

139

125

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During evolution, mammals have evolved a powerful innate immune response to LPS. Chickens are much more resistant to LPS-induced septic shock. Herein we report that chickens sense LPS via orthologs of mammalian TLR4 and myeloid differentiation protein-2 (MD-2) rather than the previously implicated chicken TLR2 isoform type 2 (chTLR2t2) receptor. Cloning and expression of recombinant chTLR4 and chMD-2 in HeLa 57A cells activated NF-κB at concentrations of LPS as low as 100 pg/ml. Differential pairing of chicken and mammalian TLR4 and MD-2 indicated that the protein interaction was species-specific in contrast to the formation of functional human and murine chimeric complexes. The chicken LPS receptor responded to a wide variety of LPS derivatives and to the synthetic lipid A compounds 406 and 506. The LPS specificity resembled the functionality of the murine rather than the human TLR4/MD-2 complex. Polymorphism in chTLR4 (Tyr383His and Gln611Arg) did not influence the LPS response. Interestingly, LPS consistently failed to activate the MyD88-independent induction of IFN-β in chicken cells, in contrast to the TLR3 agonist poly(I:C) that yielded a potent IFN-β response. These results suggest that chicken lack a functional LPS-specific TRAM-TRIF (TRIF-related adapter molecule/TIR-domain-containing adapter-inducing IFN-β) signaling pathway, which may explain their aberrant response to LPS compared with the mammalian species.

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“…In addition to a cluster of immunologically active substances, we also analysed the responses of different subpopulations of phagocytic mononuclear cells and measured cytokines produced either by mononuclear cells derived from the blood (monocytes) or the spleen (macrophages). It is possible that our cell culture contained also other cells, such as dendritic cells (Quéré et al, 2013) or thrombocytes (Scott and Owens, 2008), which may have also contributed to the overall mRNA pool. Nevertheless, blood-derived monocytes responded to LPS stimulation by a higher increase in the gene expression of proinflammatory cytokines and chemokines (especially IL-6, IL-1β and K203) than did spleen macrophages (Figs 2 and 3).…”

Section: Discussionmentioning

confidence: 99%

Preliminary results on intersexual differences in gene expression of chemokine K203 in mononuclear cells of chicken

Kankova

Zeman

Schwarz

et al. 2016

Acta Veterinaria Hungarica

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Sex steroid levels increase during sexual maturation and cause alterations in many physiological and morphological traits. Some of these changes may be connected with age-dependent and intersexual differences in the immune system. This topic is still insufficiently understood, especially in avian species, partially due to methodological limitations. In this study we measured the gene expression of proinflammatory cytokines (IL-1β, IL-6, IL-18) and chemokines [K60 (IL-8-like chicken chemokine -CXCLi1), CAF (IL-8-like chicken chemokine -CXCLi2), and K203] in mononuclear cells isolated from blood and spleen after in vitro stimulation with lipopolysaccharide (LPS). Samples were collected from chickens at two ages (from pullets before sexual maturity and from sexually mature egglaying hens). After LPS stimulation, a substantial increase was recorded in the gene expression of IL-6 and K203. All other measured genes were expressed at low levels in mononuclear cells irrespective of cell sources. We found a trend toward intersexual differences in K203 expression, but the expression of other cytokines and chemokines did not differ between the two sexes. The effect of stimulation was more pronounced in monocytes than in spleen macrophages, mainly in IL-6, IL-1β and K203 gene expression. Our findings represent a basis for further studies on the effects exerted by sexual hormones on the immune phenotype of birds.

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Thrombocytes respond to lipopolysaccharide through Toll-like receptor-4, and MAP kinase and NF-κB pathways leading to expression of interleukin-6 and cyclooxygenase-2 with production of prostaglandin E2

Cited by 89 publications

References 40 publications

Effects of Glycyrrhizic Acid on Productive and Immune Parameters of Broilers

Effects of Glycyrrhizic Acid on Productive and Immune Parameters of Broilers

Unique Properties of the Chicken TLR4/MD-2 Complex: Selective Lipopolysaccharide Activation of the MyD88-Dependent Pathway

Preliminary results on intersexual differences in gene expression of chemokine K203 in mononuclear cells of chicken

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