Thrombin potential and traditional coagulation assay

Romagnuolo, Ilaria; Attanasio, Monica; Cozzolino, Mauro; Paladino, Enrichetta; Castaman, Giancarlo; Coccia, Maria Elisabetta; Fatini, C.

doi:10.1097/mbc.0000000000000675

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…The transcription efficiency of the promoter of ANXA5 appears to be correlated to the expression of IgG antibodies against domain I (24). At present, accumulating evidence obtained from thrombo-elastography experiments and coagulation assays has shown an increased level of coagulation activity in patients with RPL (25,26). In women with RPL, the prevalence of M2 haplotype is notably increased compared with that observed in healthy controls (10).…”

Section: Discussionmentioning

confidence: 98%

Genetic analysis of ANXA5 haplotype and its effect on recurrent pregnancy loss

et al. 2021

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Recurrent pregnancy loss (RPL) is often associated with dysregulated Annexin A5 (ANXA5) expression. Moreover, the variants of Anxa5, a protein that is enriched in the placenta to prevent coagulation, have been reported to affect RPL risks. The haplotypes M1 [including single nucleotide polymorphisms (SNPs) 1A/C and 27T/C] and M2 (including SNPs 19G/A, 1A/C, 27T/C and 76G/A) of ANXA5 were also reported to affect RPL risks. The present study aimed to investigate the association between the haplotype located in the promoter region of ANXA5 and the risk of RPL. Patients with RPL (n=235) or intrauterine fetus death (IUFD; n=154), as well as healthy control subjects (n=375) were enrolled in the current research. Their haplotypes of ANXA5 were determined using genotyping, and the association between ANXA5 haplotypes and the risk of RPL was accordingly analyzed. A luciferase assay was conducted to investigate the haplotype responsible for ANXA5 activity. Reverse transcription-quantitative PCR, western blot analysis, immunohistochemistry and ELISA were performed to assess the expression level and activity of ANXA5 in patients with RPL. Consequently, the majority (n=214) of patients with RPL had a history of early RPL, whereas 31 patients with RPL had a history of both early and late RPL episodes. A significant difference was found between cases and controls in terms of gravidity and parity, whereas no significant differences were found in terms of age. The percentage of patients with RPL carrying the M2 haplotype of ANXA5 was significantly higher compared with that in control subjects, indicating that the M2 haplotype of ANXA5 was an independent risk of RPL as it influenced the transcription efficiency of ANXA5 promoter. In patients with RPL, ANXA5 activity was suppressed and the mRNA and protein expression levels of Anxa5 were decreased. Thus, the ANXA5 M2 haplotype may be an independent risk factor of RPL by affecting Anxa5 activity.

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Section: Discussionmentioning

confidence: 98%

Genetic analysis of ANXA5 haplotype and its effect on recurrent pregnancy loss

et al. 2021

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“…Romagnuolo [7] found significantly higher fibrinogen, F VIII, and plasminogen activator inhibitor 1 levels, and thrombin generation, in patients with recurrent pregnancy losses, with respect to those observed in patients with uneventful pregnancies. No fetal loss occurred in our study subjects.…”

Section: Discussionmentioning

confidence: 99%

Coagulation tests and blood glucose before vaginal delivery in healthy teenage pregnant women compared with healthy adult pregnant women

Covali¹,

Socolov²,

Socolov³

2019

Medicine

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The aim of this study was to compare the coagulation tests and blood glucose levels between healthy teenage pregnant patients and healthy adult pregnant patients just before vaginal delivery In a prospective study, 208 consecutive patients, 3rd trimester healthy pregnant women, underwent blood tests to determine their glucose levels the day before vaginal delivery. Of the 208 patients, 103 also underwent blood coagulation testing performed on the same day. The median values of the coagulation tests (APTT, prothrombin time, INR, prothrombin activity) and blood glucose were very similar in the healthy pregnant teenagers (32.6; 12.9; 1.02; 97.1; 81) compared with that in the healthy adult pregnant patients (32.45; 13.1; 1.01; 97.5; 81.2). Only the median value for fibrinogen was significantly different in healthy pregnant teenagers (348.9 mg/dL) (interquartile range 21.7) compared with that in healthy adult pregnant patients (359.1 mg/dL) (interquartile range 29.88). Significantly different median blood glucose levels also occurred in the <20; 20–29; 30–39; >40 age groups, but the glucose levels were still within normal limits. Even if there was variability between blood values from one age group to another, the median values for coagulation tests and blood glucose were very close in the healthy teenage pregnant patients compared with the median values of the healthy adult pregnant patients, just before vaginal delivery. With very few exceptions, the values for coagulation tests and blood glucose were within normal limits in all age groups of healthy pregnant patients.

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A label-free electrochemical aptasensor based on magnetic biocomposites with Pb2+-dependent DNAzyme for the detection of thrombin

Zhu

et al. 2019

Analytica Chimica Acta

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Thrombin potential and traditional coagulation assay

Cited by 6 publications

References 22 publications

Genetic analysis of ANXA5 haplotype and its effect on recurrent pregnancy loss

Genetic analysis of ANXA5 haplotype and its effect on recurrent pregnancy loss

Coagulation tests and blood glucose before vaginal delivery in healthy teenage pregnant women compared with healthy adult pregnant women

A label-free electrochemical aptasensor based on magnetic biocomposites with Pb2+-dependent DNAzyme for the detection of thrombin

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