Thrombin Inhibition: Preliminary Assessment of the Anticoagulant Potential of <i>Turnera subulata</i> (Passifloraceae)

Luz, Jefferson Romáryo Duarte da; Nascimento, Thayse Evellyn Silva do; Morais, Leandro Vinicius Fernandes de; Cruz, Ana Katarina Menezes da; Rezende, Adriana Augusto de; Neto, José Brandão; Ururahy, Marcela Abbott Galvão; Luchessi, André Ducati; López, Jorge A.; Rocha, Hugo Alexandre Oliveira; Almeida, Maria das Graças

doi:10.1089/jmf.2018.0141

Cited by 8 publications

(11 citation statements)

References 30 publications

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“…Thrombin can signal the expression of proinflammatory cytokines, such as IL-1β and IL-6, and cell adhesion molecules, promoting leukocyte activation and fibroblast proliferation, as well as contributing to leukocyte recruitment [ 65 , 66 ]. The studies with T. subulata ethanol and ethyl acetate leaf extracts demonstrated a direct inhibitory effect on thrombin and an indirect one through heparin cofactor II, corroborating the effect of the extracts on the inflammatory process [ 26 ].…”

Section: Discussionmentioning

confidence: 77%

“…In addition to the immunomodulatory effects demonstrated in this study, it is noteworthy that Luz et al [ 26 ] showed the ability of T. subulata extracts to inhibit thrombin, the main protease in the coagulation cascade, which plays a role in the inflammatory response [ 65 ]. Thrombin can signal the expression of proinflammatory cytokines, such as IL-1β and IL-6, and cell adhesion molecules, promoting leukocyte activation and fibroblast proliferation, as well as contributing to leukocyte recruitment [ 65 , 66 ].…”

Section: Discussionmentioning

confidence: 97%

“…Thus, this study presented the chemical profile characterization of aqueous and hydroethanolic extracts of T. subulata flower and leaf by mass spectrometry, as well as the evaluation of their anti-inflammatory properties in an in vitro model. At this point, it is noteworthy that only a few studies have reported the presence of phenolic compounds especially in T. subulata flowers, as well as their pharmacological properties [ 25 , 26 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding T. subulata , their anti-inflammatory effects have mainly been described by inhibiting the cytokine production [ 25 ]. In a previous study, Luz et al [ 26 ] showed the ability of T. subulata extracts to directly and indirectly inhibit thrombin, promoting few side-effects. These data are relevant since this coagulation factor is also related to an inflammatory response [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Characterization of Flowers and Leaf Extracts Obtained from Turnera subulata and Their Immunomodulatory Effect on LPS-Activated RAW 264.7 Macrophages

et al. 2022

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The anti-inflammatory properties of Turnera subulata have been evaluated as an alternative drug approach to treating several inflammatory processes. Accordingly, in this study, aqueous and hydroalcoholic extracts of T. subulata flowers and leaves were analyzed regarding their phytocomposition by ultrafast liquid chromatography coupled to mass spectrometry, and their anti-inflammatory properties were assessed by an in vitro inflammation model, using LPS-stimulated RAW-264.7 macrophages. The phytochemical profile indicated vitexin-2-O-rhamnoside as an important constituent in both extracts, while methoxyisoflavones, some bulky amino acids (e.g., tryptophan, tyrosine, phenylalanine), pheophorbides, and octadecatrienoic, stearidonic, and ferulic acids were detected in hydroalcoholic extracts. The extracts displayed the ability to modulate the in vitro inflammatory response by altering the secretion of proinflammatory (TNF-α, IL-1β, and IL-6) and anti-inflammatory (IL-10) cytokines and inhibiting the PGE-2 and NO production. Overall, for the first time, putative compounds from T. subulata flowers and leaves were characterized, which can modulate the inflammatory process. Therefore, the data highlight this plant as an option to obtain extracts for phytotherapic formulations to treat and/or prevent chronic diseases.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chemical Characterization of Flowers and Leaf Extracts Obtained from Turnera subulata and Their Immunomodulatory Effect on LPS-Activated RAW 264.7 Macrophages

et al. 2022

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“…Besides, it presented a similar effect to the L. furfuracea extract, but unlike this, the heparin concentrations were less than 1 µg/mL (Table 2). In this study, the ATTP was prolonged > 300 s from 0.4 µg/mL of heparin, while the fractions of polyphenolic-polysaccharide isolated from the medicinal plant Erigeron canadensis L. required 390 µg/mL, and the Unfractionated Heparin 1 µg/mL prolonged this same time [71]. On the other hand, the polyphenolic fractions of Sargassum sp.…”

Section: Effect Of Hydroethanolic Extract On Clotting Timesmentioning

confidence: 60%

Laelia furfuracea Lindl.: an Endemic Mexican Orchid with Anticoagulant Activity

et al. 2021

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Abstract. Laelia furfuracea is an endemic orchid from Mexico, with antioxidant activity. The objective of this study was to evaluate the effect of hydroethanolic extract and fractions obtained from the orchid leaves on the clotting times of patients with venous thromboembolic disease (VTD) and to identify their tentative compounds. The anticoagulant activity was evaluated by determining prothrombin (PT), thrombin (TT) and, activated partial thromboplastin (APTT) times. Identification of the compounds was carried out using a chromatographic technique with an ultra-high-performance liquid chromatographic analyzer coupled with electrospray ionization with quadrupole time of flight-mass-mass spectrometry. The extract prolonged the clotting times depending on the concentration-response (5-60 mg / mL); 25 mg/mL prolonged the PT (33.2 ± 2.3 s) and TT (33.1 ± 0.3 s); and APTT (61.8 ± 3.4 s) at a concentration of 15 mg/mL. The main groups tentatively identified were xanthine, carboxylic acid, amino acid, and phenolic compounds. These compounds or the synergy between them prolong clotting times. Laelia furfuracea is an orchid with research potential in the search for new anticoagulant agents. Resumen. Laelia furfuracea es una orquídea endémica de México, la cual posee actividad antioxidante. El objetivo de este estudio fue evaluar el efecto del extracto hidroetanólico y fracciones obtenidas de hojas de la orquídea sobre los tiempos de coagulación de pacientes con enfermedad tromboembólica venosa (ETV) e identificar sus posibles compuestos. La actividad anticoagulante se evaluó determinando los tiempos de protrombina (TP), trombina (TT) y tromboplastina parcial activada (TTPA). La identificación de los compuestos se realizó usando una técnica cromatográfica con un analizador cromatográfico líquido de Ultra Alta Resolución con Ionización por electroespray acoplado a espectrometría de masas con Cuadrupolo y Tiempo de Vuelo. El extracto prolongó los tiempos de coagulación dependiente de la concentración-respuesta (5-60 mg/mL); 25 mg/mL prolongó el TP (33.2±2.3 s) y TT (33.1±0.3 s); y TTPA (61.8±3.4 s) a una concentración de 15 mg/mL. Los principales grupos de posibles compuestos identificados fueron xantina, ácido carboxílico, aminoácido y compuestos fenólicos. Estos compuestos o la sinergia entre ellos prolongan los tiempos de coagulación. Laelia furfuracea es una orquídea con potencial en investigación para la búsqueda de nuevos agentes anticoagulantes.

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Licania rigida Benth leaf extracts: Assessment of toxicity and potential anticoagulant effect

Luz

Nascimento

Araújo-Silva

et al. 2021

South African Journal of Botany

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Thrombin Inhibition: Preliminary Assessment of the Anticoagulant Potential of Turnera subulata (Passifloraceae)

Cited by 8 publications

References 30 publications

Chemical Characterization of Flowers and Leaf Extracts Obtained from Turnera subulata and Their Immunomodulatory Effect on LPS-Activated RAW 264.7 Macrophages

Chemical Characterization of Flowers and Leaf Extracts Obtained from Turnera subulata and Their Immunomodulatory Effect on LPS-Activated RAW 264.7 Macrophages

Laelia furfuracea Lindl.: an Endemic Mexican Orchid with Anticoagulant Activity

Licania rigida Benth leaf extracts: Assessment of toxicity and potential anticoagulant effect

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