Thrombin generation by intimal tissue factor contributes to thrombus formation on macrophage-rich neointima but not normal intima of hyperlipidemic rabbits

Yamashita, Atsushi; Matsuda, Shuntaro; Matsumoto, Tomoko; Moriguchi-Goto, Sayaka; Takahashi, Masahiro; Sugita, Chihiro; Sumi, Takahiro; Imamura, Takuroh; Shima, Midori; Kïtamura, Kazuo; Asada, Yujiro

doi:10.1016/j.atherosclerosis.2009.03.014

Cited by 39 publications

(53 citation statements)

References 26 publications

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“…The thrombus size in the normal femoral artery was significantly smaller than that in the injured (atherosclerotic) artery in rabbits fed with 0.5% cholesterol diets, and did not differ from that in the femoral artery of rabbits fed with 0.5% cholesterol diet. 15 …”

Section: Discussionmentioning

confidence: 99%

“…Areas of positive immunostaining in the vessel and thrombus were analyzed using a color imaging morphometry system (Win Roof, Mitani, Fukui, Japan). 15 The data are expressed as ratios of the immunopositive area (μm 2 ), or as numbers of immunopositive nuclei/mm 2 .…”

Section: Histologic Analysismentioning

confidence: 99%

“…After 18 F-FDG-PET imaging, a 2F balloon catheter (Baxter Healthcare) was inserted via the anterior tibial arteries into both iliacofemoral arteries, inflated to 1.4 atm, and retracted twice to induce arterial thrombus; 15 15 min later, the rabbits were injected with heparin (500 U/kg, i.v.) and then killed 5 min later with an overdose of pentobarbital (60 mg/kg, i.v.).…”

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confidence: 99%

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Arterial <sup>18</sup>F-Fluorodeoxyglucose Uptake Reflects Balloon Catheter-Induced Thrombus Formation and Tissue Factor Expression via Nuclear Factor-κB in Rabbit Atherosclerotic Lesions

Yamashita

Zhao

Matsuura

et al. 2013

Circ J

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Section: Discussionmentioning

confidence: 99%

Section: Histologic Analysismentioning

confidence: 99%

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confidence: 99%

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Arterial <sup>18</sup>F-Fluorodeoxyglucose Uptake Reflects Balloon Catheter-Induced Thrombus Formation and Tissue Factor Expression via Nuclear Factor-κB in Rabbit Atherosclerotic Lesions

Yamashita

Zhao

Matsuura

et al. 2013

Circ J

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“…7 In animal models, endothelial denudation without medial injury is associated with platelet deposition without fibrin, whereas fibrin is observed with more severe injury that damages the media. 8,9 These results suggest that exposure of adventitial TF is required for activation of blood coagulation and fibrin deposition. Surprisingly, deletion of the TF gene in VSMCs in mice reduced ferric chloride-induced thrombosis.…”

Section: Article See P 365mentioning

confidence: 72%

Uremic Serum and Ubiquitylation of Tissue Factor

Mackman

2013

Circulation

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“…Data are expressed as percentage of immunopositive areas in the venous wall. 20) Two investigators (A.Y. and Y.A.)…”

Section: Preparation Of Blood Vessels and Contractile Studiesmentioning

confidence: 99%

Relative Contributions of 5-Hydroxytryptamine (5-HT) Receptor Subtypes in 5-HT-Induced Vasoconstriction of the Distended Human Saphenous Vein as a Coronary Artery Bypass Graft

Nakamura

Tanaka

Kuwabara³

et al. 2011

Biological & Pharmaceutical Bulletin

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Coronary artery bypass graft (CABG) surgery has been performed for about 40 years. In 1967, the technique of saphenous vein (SV) bypass grafting was introduced, followed 1 year later by the use of internal thoracic artery (ITA).1) Whereas ITA is the first-choice arterial graft, 2,3) SV still continues to be the most commonly used conduit vessel for CABG 4,5) because of ready availability and suppleness. One of the most serious problems in CABG surgery is that the bypass grafts often go into spasm after implantation into coronary arterial circulation, which has led to premature occlusion and increased perioperative morbidity. 6,7) Among substances that can initiate severe vasoconstriction, 5-hydroxytryptamine (5-HT) has been identified as playing an important role in the pathogenesis of vascular spasm. [8][9][10][11][12][13] Although 5-HT 2A and/or 5-HT 1B/1D receptor subtypes almost exclusively mediate 5-HT-induced vasoconstriction, [8][9][10][11][12][13][14][15][16][17][18] the relative contributions of these receptor subtypes to vasoconstrictions are species-dependent and vascular beddependent. Therefore, the selection of the best pharmacological agent to prevent 5-HT-induced spasm of the bypass grafts used in CABG surgery requires better understanding of the contributions of 5-HT receptor subtypes to vasoconstriction in the clinically used human vessels.From the viewpoint mentioned above, we revealed that 5-HT-induced vasoconstriction is mediated equivalently via 5-HT 2A and 5-HT 1B receptors in the human ITA.18) In the human SV, Borton et al. 14) have reported that both 5-HT 1 and 5-HT 2 receptors are involved in vasoconstriction by using a 5-HT 2 receptor antagonist and a 5-HT 1 receptor agonist. However, the relative contributions of 5-HT receptor subtypes that mediate vasoconstriction caused by 5-HT in the human SV are still unclear.It has been established that, before implantation into the coronary arterial circulation, the segment of SV used as a vein graft is distended by applying high intraluminal pressures with injection of saline or a patient's heparinized blood to check for leaks and to increase the vascular diameter. This fact should be considered in experiments for evaluation of 5-HT receptor subtypes responsible for vascular functions because the distension may influence both contractile and dilatory responses of the SV. 19)The aim of the present study was to elucidate the relative contribution of 5-HT receptor subtypes mediating 5-HTinduced vasoconstriction of the distended human SV obtained from CABG surgery. We examined the effects of sarpogrelate and SB224289, antagonists of 5-HT 2A and 5-HT 1B receptors, respectively, on the 5-HT-induced vasoconstriction and also performed immunolabeling of the 5-HT 2A and 5-HT 1B receptors in the distended SV. MATERIALS AND METHODS Preparation of Blood Vessels and Contractile StudiesSegments of the human SV were harvested from patients (nϭ12) who were undergoing CABG surgery at Miyazaki Prefectural Nobeoka Hospital (Miyazaki, Japan). Once the vein was comp...

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Thrombin generation by intimal tissue factor contributes to thrombus formation on macrophage-rich neointima but not normal intima of hyperlipidemic rabbits

Cited by 39 publications

References 26 publications

Arterial <sup>18</sup>F-Fluorodeoxyglucose Uptake Reflects Balloon Catheter-Induced Thrombus Formation and Tissue Factor Expression via Nuclear Factor-κB in Rabbit Atherosclerotic Lesions

Arterial <sup>18</sup>F-Fluorodeoxyglucose Uptake Reflects Balloon Catheter-Induced Thrombus Formation and Tissue Factor Expression via Nuclear Factor-κB in Rabbit Atherosclerotic Lesions

Uremic Serum and Ubiquitylation of Tissue Factor

Relative Contributions of 5-Hydroxytryptamine (5-HT) Receptor Subtypes in 5-HT-Induced Vasoconstriction of the Distended Human Saphenous Vein as a Coronary Artery Bypass Graft

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