Thrombin Generation Biomarkers Decline With Parenteral Anticoagulation—An Overlooked Means of Anticoagulation Monitoring?

McFarland, Craig; Lind, Stuart E.

doi:10.1177/1076029617746506

Cited by 5 publications

(2 citation statements)

References 74 publications

(70 reference statements)

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“…This is believed to be partly caused by changes in the structure of the resulting fibrin clots [30]. In addition, all thrombin generation markers (F1+2, TAT, and D-dimer) [31] have been reported to be elevated in patients with thrombosis [32]. Indeed, in a cohort of cancer patients (n = 821) it was observed that elevated levels of F1+2 and D-dimer were associated with the occurrence of thromboembolic incidents [33].…”

Section: Discussionmentioning

confidence: 99%

Coagulation Changes during Central Hypovolemia across Seasons

Goswami

Taucher

Brix

et al. 2020

JCM

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Lower body negative pressure (LBNP) application simulates hemorrhage. We investigated how seasons affect coagulation values at rest and during LBNP. Healthy participants were tested in cold (November–April) and warm (May–October) months. Following a 30-min supine period, LBNP was started at −10 mmHg and increased by −10 mmHg every five minutes until a maximum of −40 mmHg. Recovery was for 10 min. Blood was collected at baseline, end of LBNP, and end of recovery. Hemostatic profiling included standard coagulation tests, calibrated automated thrombogram, thrombelastometry, impedance aggregometry, and thrombin formation markers. Seven men (25.0 ± 3.6 years, 79.7 ± 7.8 kg weight, 182.4 ± 3.3 cm height, and 23.8 ± 2.3 kg/m2 BMI) and six women (25.0 ± 2.4 years, 61.0 ± 8.4 kg weight, 167 ± 4.7 cm height, and 21.8 ± 2.4 kg/m2 BMI) participated. Baseline levels of prothrombin (FII), tissue factor (TF) and markers for thrombin generation F1+2 and the thrombin/antithrombin complex (TAT) were higher during summer. Factor VIII, prothrombin fragment 1+2 (F1+2), TAT and the coagulation time showed significant increases during LBNP in both seasons. Some calibrated automated thrombography variables (Calibrated automated thrombography (CAT): lag, time to peak (ttPeak), peak) shifted in a procoagulant direction during LBNP in summer. Red blood cell counts (RBC), hemoglobin and white blood cell counts (WBC) decreased during LBNP. LBNP application reduced prothrombin time in winter and activated partial thromboplastin time in summer. Greater levels of FII, TF, F1+2, and TAT—a more pronounced LBNP-induced procoagulative effect, especially in CAT parameters (lag time (LT), Peak, ttPeak, Velindex)—were seen in summer. These results could have substantial medical implications.

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Section: Discussionmentioning

confidence: 99%

Coagulation Changes during Central Hypovolemia across Seasons

Goswami

Taucher

Brix

et al. 2020

JCM

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“…Уровни D-димера позитивно ассоциированы с окисленными фосфолипидами apoB-содержащих липопротеинов, некрозом, эрозией и разрывом каротидной бляшки [69], риском инсульта при острой сердечной недостаточности [18], мерцательной аритмией [136], тромбозом глубоких вен [88], риском венозного тромбоэмболизма у пациентов с злокачественными заболеваниями системы крови [110]. D-димер -показатель генерации тромбина и адекватности антикоагулянтной терапии [87].…”

Section: факторы ангиогенезаunclassified

Biomarkers of Cardiovascular Disease

Scherbak

Григорьевич²,

Лисовец

et al. 2019

Physical and rehabilitation medicine, medical rehabilitation

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Inflammation, cardiac remodeling, and fibrosis are potentially important pathways in the pathogenesis of cardiovascular diseases. Complications of atherosclerosis are one of the leading causes of death in the world. Effective prevention of cardiovascular disease by adequate control of major cardiovascular risk factors can provide substantial public health gains. However, detection and control of major cardiovascular risk factors continues to be a major challenge because of poor awareness of an individual's status. A solution to this problem is important for an early identification and appropriate correction of cardiovascular risk factors. Atherosclerotic plaque development is regarded as a chronic inflammatory process which involves interactions between lipids, immune cells and the artery wall. Numerous evidence suggests that inflammation plays an important role in all stages of the atherosclerotic process. The study of associations of inflammatory biomarkers has led to the idea that the panel of inflammatory biomarkers can identify people at high risk of developing atherosclerosis and cardiovascular diseases when anti-inflammatory treatment can prevent further unfavorable events. The most common forms of cardiovascular diseases are caused by atherosclerosis, the progressive thinning of blood vessels due to accumulation of lipids within the arterial wall. While many factors are known to influence the development and progression of atherosclerosis, circulating levels of cholesterol and lipoprotein complexes are the most important risk factors and mediators of atherosclerotic disease. Key regulators of lipid metabolism and/or the development of atherosclerosis have diagnostic, prognostic and therapeutic potential for cardiovascular diseases.

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Heparin-Bead Extraction Enhanced Fluorogenic Aptamer–Thrombin Composite Reporter Enables Sensitive and Rapid Detection of Functional Antithrombin

Yan,

Tian,

Ziegenbein

et al. 2024

ACS Sens.

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Antithrombin (AT) deficiency in the extracorporeal circulation during cardiac surgery leads to uncontrolled inflammation and vascular damage in patients. AT levels decrease in sepsis, major trauma, extracorporeal membrane oxygenation, and eclampsia. Monitoring plasma AT levels facilitates the accurate restoration of AT to baseline values through precise supplementation. Traditional methods of chromogenic assay and enzyme-linked immunosorbent assay (ELISA) kits encounter challenges, such as interference, inconsistency, and delayed response times, making real-time, reliable antithrombin monitoring a clinical gap. To address this critical need, we develop a heparin-bead extraction enhanced fluoroGenic aptamer−thrombin composite reporter (HExGATOR) for the rapid, sensitive, and precise detection of functional AT in plasma. Our design employs thrombinbinding aptamers and a fluorescence "turn on" technology such that a signal is produced upon the interaction of AT with the otherwise "turned off" aptamer−thrombin complex. The prominent signal-background interference originating from plasma is remarkably diminished by using a heparin-bead solid-phase extraction of AT. We achieved highly sensitive and rapid detection of AT in 5 to 20 min with a limit of detection of 15.11 nM. This approach offers a promising alternative to traditional AT tests in clinical settings, potentially facilitating personalized anticoagulant therapy.

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Thrombin Generation Biomarkers Decline With Parenteral Anticoagulation—An Overlooked Means of Anticoagulation Monitoring?

Cited by 5 publications

References 74 publications

Coagulation Changes during Central Hypovolemia across Seasons

Coagulation Changes during Central Hypovolemia across Seasons

Biomarkers of Cardiovascular Disease

Heparin-Bead Extraction Enhanced Fluorogenic Aptamer–Thrombin Composite Reporter Enables Sensitive and Rapid Detection of Functional Antithrombin

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