Thrombin Augments Vascular Cell-dependent Migration of Human Mast Cells: Role of MGF

Baghestanian, Mehrdad; Hofbauer, Roland; Kress, H. G.; Wojta, Johann; Fabry, Astrid; Binder, Bernd R.; Kaun, Christoph; Müller, Michael; Mehrabi, Mohammad Reza; Kapiotis, Stylianos; Sengoelge, Gürkan; Ghannadan, Minoo; Lechner, Klaus; Valent, Peter

doi:10.1055/s-0038-1656008

Cited by 26 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with previous data (28), rHuSCF induced migration of HMC-1 in a dose-dependent manner ( Figure 5B). An antibody against SCF was found to block both SFB supernatant-induced ( Figure 5A) and SCF-induced ( Figure 5C) migration of HMC-1, but did not inhibit rHuC5a-induced HMC-1 migration (results not shown).…”

Section: Resultssupporting

confidence: 93%

“…SCF is expressed in certain microenvironmental cells, including fibroblasts and endothelial cells (26)(27)(28). The mature protein is found in either membrane-bound or soluble form.…”

Section: Sion and Release Of Scf Recombinant Human Scf (Rhuscf) And mentioning

confidence: 99%

“…The migration of HMC-1 cells (human mast cell line) against rHuSCF or against SFB supernatant was determined by a double-chamber chemotaxis assay as described previously (28). In this assay, SFB together with conditioned medium, rHuSCF (0.001-100 ng/ml), or control medium (RPMI 1640 plus 10% FCS) were placed in the lower chambers of the wells.…”

Section: Kiener Et Almentioning

confidence: 99%

See 2 more Smart Citations

Tumor necrosis factor α promotes the expression of stem cell factor in synovial fibroblasts and their capacity to induce mast cell chemotaxis

Kiener

Hofbauer

Tohidast‐Akrad

et al. 2000

Arthritis & Rheumatism

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 93%

“…SCF is expressed in certain microenvironmental cells, including fibroblasts and endothelial cells (26)(27)(28). The mature protein is found in either membrane-bound or soluble form.…”

Section: Sion and Release Of Scf Recombinant Human Scf (Rhuscf) And mentioning

confidence: 99%

Section: Kiener Et Almentioning

confidence: 99%

See 1 more Smart Citation

Tumor necrosis factor α promotes the expression of stem cell factor in synovial fibroblasts and their capacity to induce mast cell chemotaxis

Kiener

Hofbauer

Tohidast‐Akrad

et al. 2000

Arthritis & Rheumatism

Self Cite

View full text Add to dashboard Cite

show abstract

“…Histamine, proteases, TNF and PGs are known to induce vasodilation and increase vascular permeability [68]. Endothelial cells release IL-3 and thrombin, which enhance migration, proliferation and local differentiation of mast cells [69]. Mast cells increase fivefold along wound edges, an event caused by chemokine monocyte chemoattractant protein-1 and transforming growth factor (TGF)-β, a vital wound healing cytokine, which is also a potent chemoattractant for mast cells [70].…”

Section: Mast Cell Stabilisation and Wound Healingmentioning

confidence: 99%

A review of olopatadine for the treatment of ocular allergy

Abelson¹

2004

Expert Opinion on Pharmacotherapy

View full text Add to dashboard Cite

Ocular allergy affects > 20% of the general population and many therapeutic preparations are available to individuals experiencing the most common forms--seasonal and perennial allergic conjunctivitis. 0.1% Olopatadine topical ophthalmic solution is currently approved for the treatment of allergic signs and symptoms in patients > or = 3 years of age. Olopatadine is available in Europe as Opatanol) and in > 30 other countries as Patanol. It inhibits mast cell degranulation and antagonises histamine receptors to manage the itching, redness, chemosis, tearing and lid swelling of the ocular allergic reaction, and its mast cell stabilising ability has been demonstrated both in vitro (using human conjunctival mast cells) and in vivo (human clinical experience). This article reviews both the laboratory and clinical information available on olopatadine, prefaced by a discussion of the current understanding of the ocular allergic reaction and followed by the future implications for this compound. Both laboratory and clinical studies have established the efficacy, safety and comfort of olopatadine in several study design models and comparisons to other antiallergy medications. The application of olopatadine, specifically in the management of lid swelling, an allergic sign recalcitrant to therapy and nasal allergic symptoms has also been established. In the future, a new formulation containing 0.2% olopatadine exhibits a duration of action up to 24 h, supporting once-daily dosing.

show abstract

“…PAR-1 has been shown to be active in the release of IL-6 from murine mast cells (13) and NO from rat peritoneal mast cells (14). Thrombin may also affect mast cell biology through the release of mast cell chemotactic factors from endothelial cells (15).…”

mentioning

confidence: 99%

Thrombin Induces Mast Cell Adhesion to Fibronectin: Evidence for Involvement of Protease-Activated Receptor-1

Vliagoftis

2002

The Journal of Immunology

View full text Add to dashboard Cite

Thrombin activates mast cells to release inflammatory mediators through a mechanism involving protease-activated receptor-1 (PAR-1). We hypothesized that PAR-1 activation would induce mast cell adhesion to fibronectin (FN). Fluorescent adhesion assay was performed in 96-well plates coated with FN (20 μg/ml). Murine bone marrow cultured mast cells (BMCMC) were used after 3–5 wk of culture (>98% mast cells by flow cytometry for c-Kit expression). Thrombin induced β-hexosaminidase, IL-6, and matrix metalloproteinase-9 release from BMCMC. Thrombin and the PAR-1-activating peptide AparafluoroFRCyclohexylACitY-NH2 (cit) induced BMCMC adhesion to FN in a dose-dependent fashion, while the PAR-1-inactive peptide FSLLRY-NH2 had no effect. Thrombin and cit induced also BMCMC adhesion to laminin. Thrombin-mediated adhesion to FN was inhibited by anti-α5 integrin Ab (51.1 ± 6.7%; n = 5). The combination of anti-α5 and anti-α4 Abs induced higher inhibition (65.7 ± 7.1%; n = 5). Unlike what is known for FcεRI-mediated adhesion, PAR-1-mediated adhesion to FN did not increase mediator release. We then explored the signaling pathways involved in PAR-1-mediated mast cell adhesion. Thrombin and cit induced p44/42 and p38 phosphorylation. Pertussis toxin inhibited PAR-1-mediated BMCMC adhesion by 57.3 ± 7.3% (n = 4), indicating that Gi proteins are involved. Wortmannin and calphostin almost completely inhibited PAR-1-mediated mast cell adhesion, indicating that PI-3 kinase and protein kinase C are involved. Adhesion was partially inhibited by the mitogen-activated protein kinase kinase 1/2 inhibitor U0126 (24.5 ± 3.3%; n = 3) and the p38 inhibitor SB203580 (25.1 ± 10.4%; n = 3). The two inhibitors had additive effects. Therefore, thrombin mediates mast cell adhesion through the activation of Gi proteins, phosphoinositol 3-kinase, protein kinase C, and mitogen-activated protein kinase pathways.

show abstract

Thrombin Augments Vascular Cell-dependent Migration of Human Mast Cells: Role of MGF

Cited by 26 publications

References 27 publications

Tumor necrosis factor α promotes the expression of stem cell factor in synovial fibroblasts and their capacity to induce mast cell chemotaxis

Tumor necrosis factor α promotes the expression of stem cell factor in synovial fibroblasts and their capacity to induce mast cell chemotaxis

A review of olopatadine for the treatment of ocular allergy

Thrombin Induces Mast Cell Adhesion to Fibronectin: Evidence for Involvement of Protease-Activated Receptor-1

Contact Info

Product

Resources

About