Thrips as the Vectors of Tospoviruses in Indian Agriculture

Ghosh, Amalendu; Dey, Debjani; Timmanna,; Basavaraj,; Mandal, Bikash; Jain, Rakesh

doi:10.1007/978-981-10-5672-7_24

Cited by 24 publications

(18 citation statements)

References 70 publications

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“…From Goiás state, only 11 species of Thysanoptera have been cataloged , including four belonging to the genus Frankliniella, i.e., F. graminis Cavalleri and Mound, F. zucchini Nakahara & Monteiro, F. schultzei Trybom, and F. condei John (LIMA, 2019). This genus contains species that transmit viruses, specifically Tospoviruses, and thus are considered key pests of agricultural importance (GHOSH et al, 2017). The small number of Frankliniella individuals collected may result from the preference of species of this genus for flowers (ALVES-SILVA et al, 2013), a plant organ not analyzed in this study.…”

Section: Resultsmentioning

confidence: 97%

Thrips species associated with varieties of the native cerrado fruit tree Hancornia speciosa

et al. 2019

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Studies of insects associated with the fruit tree Hancornia speciosa Gomes (Apocynaceae), a native of the Brazilian Cerrado popularly known as “mangabeira”, are generally restricted to reports from seedling nurseries. Thrips predominate among insects that attack this crop. This study investigated the species of Thysanoptera that use this native fruit as a host, and which variety they prefer. The H. speciosa varieties speciosa s.str., cuyabensis, gardneri and pubescens were obtained from the collections of the ex-situ germplasm bank of native species at the Escola de Agronomia, Universidade Federal de Goiás, Goiânia, Brazil. Evaluations were conducted from January through December 2016. A total of 1679 individual thrips were identified. Thrips were most abundant on pubescens (79.08%), followed by gardneri (15.02%), cuyabensis (4.71%), and speciosa (1.19%). Representatives of the family Thripidae predominated in all collections, especially Coremothrips nubiculus and Heliothrips longisensibilis. The foliar trichomes present in var. pubescens may account for the high abundance of thrips on this variety. Heliothrips longisensibilis is recorded in Brazil for the first time.

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Section: Resultsmentioning

confidence: 97%

Thrips species associated with varieties of the native cerrado fruit tree Hancornia speciosa

et al. 2019

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“…In India, four thrips vectors viz. T. palmi, S. dorsalis, T. tabaci, and F. schultzei have been reported so far to transmit five tospoviruses such as groundnut bud necrosis virus (GBNV), watermelon bud necrosis virus (WBNV), peanut yellow spot virus (PYSV), capsicum chlorosis virus (CaCV) and Irish yellow spot virus (IYSV) [4]. Recently F. occidentalis, a potential vector of TSWV has been reported from the Nilgiri hills of India [9,10].…”

Section: Discussionmentioning

confidence: 99%

“…Tospoviruses like tomato spotted wilt virus (TSWV) infects more than 900 plant species and impatiens necrotic spot virus (INSV) can be found in over 300 plant species [1]. To date, 16 thrips vectors transmitting more than 29 tospoviruses have been reported [1][2][3][4]. Among them, four thrips vectors viz.…”

Section: Introductionmentioning

confidence: 99%

“…Among them, four thrips vectors viz. Thrips palmi Karny [5,6], Scirtothrips dorsalis Hood [7], T. tabaci Lindeman [8], and Frankliniella schultzei Trybom [7] are reported to transmit five tospoviruses in India [4]. Although F. occidentalis (Pergande) has been recently reported in India [9,10], its distribution and role in spreading tospoviruses under Indian conditions are yet to be studied.…”

Section: Introductionmentioning

confidence: 99%

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A multiplex PCR assay for rapid identification of major tospovirus vectors reported in India

et al. 2020

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Background: To date, four thrips vectors have been reported to transmit five different tospoviruses in India. Their identification at an early stage is crucial in formulating appropriate pest management strategies. Since morphometric key-based thrips identification based on the adult stage is time-consuming, there is a need to develop diagnostic tools which are rapid, accurate, and independent of developmental stages. Here, we report a multiplex PCR assay to identify four major thrips vectors viz. Thrips palmi, T. tabaci, Scirtothrips dorsalis, and Frankliniella schultzei present in India. Results: Cytochrome oxidase subunit III and internal transcribed spacer region 2 were utilized to design speciesspecific primers. Of 38 pairs of primers tested, primer pairs AG35F-AG36R, AG47F-AG48R, AG87F-AG88R, and AG79F-AG80R amplified 568 bp, 713 bp, 388 bp, and 200 bp products from the DNA templates of T. palmi, S. dorsalis, T. tabaci, and F. schultzei, respectively at same PCR conditions. The specificity of the primer pairs was validated with a large number of known specimens and no cross-reactivity was observed with other thrips species. The multiplex PCR assay with a cocktail of all the four primer pairs detected four thrips vectors efficiently and could discriminate all of them concurrently in a single reaction. Conclusion: The multiplex PCR reported in this study could identify the major thrips vectors reported in India. The assay will be useful in ascertaining distribution profile of major thrips vectors, disease epidemiology, screening large samples, and quarantine.

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“…Secondary symptoms include stunting, axillary shoot proliferation, and malformation of leaflets. Thrips palmi (Thysanoptera: Thripidae) is known to vector GBNV [40,9]. Not much is known about the relationships of thrips and tospoviruses.…”

Section: Introductionmentioning

confidence: 99%

In silico analyses of molecular interactions between groundnut bud necrosis virus and its vector, Thrips palmi

Jagdale

Ghosh

2019

VirusDis.

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Groundnut bud necrosis virus (GBNV) is an economically important tospovirus transmitted by Thrips palmi (Thysanoptera: Thripidae). The current understanding of thrips-tospovirus interactions is largely based on the tomato spotted wilt virus-Frankliniella occidentalis relationship. Only limited information is available for the GBNV-T. palmi system. In the present study, available genome data of T. palmi and GBNV were used to predict the protein partners that may play a crucial role in the internalization of GBNV virions into thrips cells. Computational analyses showed that the GBNV precursor glycoprotein bears a signal peptide of 24 amino acids and a secondary cleavage site at position 434-435 separates the amino-terminal mature glycoprotein (G N) from the carboxyl-terminal glycoprotein (G C). Potential interactions of GBNV glycoproteins were predicted with T. palmi enolase, cathepsin, C-type lectin, clathrin and vacuolar ATP synthase subunit E. The in silico analyses suggested that C-type lectin is the primary cellular receptor to interact with GBNV-G N. After receptor binding, virus particles probably enter vector cells by clathrin-mediated endocytosis. This is the first in silico evidence of GBNV-T. palmi protein interaction.

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Thrips as the Vectors of Tospoviruses in Indian Agriculture

Cited by 24 publications

References 70 publications

Thrips species associated with varieties of the native cerrado fruit tree Hancornia speciosa

Thrips species associated with varieties of the native cerrado fruit tree Hancornia speciosa

A multiplex PCR assay for rapid identification of major tospovirus vectors reported in India

In silico analyses of molecular interactions between groundnut bud necrosis virus and its vector, Thrips palmi

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