Thresholds, sensitive stages and genetic variability of finger millet to high temperature stress

Opole, R. A.; Prasad, P. V. Vara; Djanaguiraman, M.; Vimala, K. Menon; Kirkham, M.B.; Upadhyaya, Hari D.

doi:10.1111/jac.12279

Cited by 26 publications

(18 citation statements)

References 62 publications

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“…The latter implied that events early during cultivation can ultimately have a strong impact on the product yield and thus process performance. Others have identified anthesis as the most heat-sensitive phase in the plant life cycle (Zinn et al, 2010; Zhou et al, 2017; Opole et al, 2018), but this is of limited relevance in molecular farming because plants are typically harvested and processed before flower development (Ma et al, 2015; Sack et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Seasonal Weather Changes Affect the Yield and Quality of Recombinant Proteins Produced in Transgenic Tobacco Plants in a Greenhouse Setting

et al. 2019

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Transgenic plants have the potential to produce recombinant proteins on an agricultural scale, with yields of several tons per year. The cost-effectiveness of transgenic plants increases if simple cultivation facilities such as greenhouses can be used for production. In such a setting, we expressed a novel affinity ligand based on the fluorescent protein DsRed, which we used as a carrier for the linear epitope ELDKWA from the HIV-neutralizing antibody 2F5. The DsRed-2F5-epitope (DFE) fusion protein was produced in 12 consecutive batches of transgenic tobacco (Nicotiana tabacum) plants over the course of 2 years and was purified using a combination of blanching and immobilized metal-ion affinity chromatography (IMAC). The average purity after IMAC was 57 ± 26% (n = 24) in terms of total soluble protein, but the average yield of pure DFE (12 mg kg−1) showed substantial variation (± 97 mg kg−1, n = 24) which correlated with seasonal changes. Specifically, we found that temperature peaks (>28°C) and intense illuminance (>45 klx h−1) were associated with lower DFE yields after purification, reflecting the loss of the epitope-containing C-terminus in up to 90% of the product. Whereas the weather factors were of limited use to predict product yields of individual harvests conducted for each batch (spaced by 1 week), the average batch yields were well approximated by simple linear regression models using two independent variables for prediction (illuminance and plant age). Interestingly, accumulation levels determined by fluorescence analysis were not affected by weather conditions but positively correlated with plant age, suggesting that the product was still expressed at high levels, but the extreme conditions affected its stability, albeit still preserving the fluorophore function. The efficient production of intact recombinant proteins in plants may therefore require adequate climate control and shading in greenhouses or even cultivation in fully controlled indoor farms.

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Section: Resultsmentioning

confidence: 99%

Seasonal Weather Changes Affect the Yield and Quality of Recombinant Proteins Produced in Transgenic Tobacco Plants in a Greenhouse Setting

et al. 2019

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“…Changes in climatic conditions in the future will not only affect the crop distribution, but also its quality. Research conducted in the USA shows that the season-long high temperature stress reduces the chlorophyll index, seed number, grain yield and harvest index; however, only a few genotypes of finger millet show tolerance with high temperature [36]. Therefore, climate change will have at least double impact on finger millet production-first, shrinkage of suitable area, and second, the reduction in grain yield.…”

Section: Future Distribution Suitability Scenarios (Stable Loss and mentioning

confidence: 99%

Potential suitable habitat of Eleusine coracana (L) Gaertn (Finger millet) under the climate change scenarios in Nepal

Luitel

Siwakoti

Joshi³

et al. 2020

Preprint

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Background: Finger millet is the fourth major crop in Nepal and is cultivated in a traditional integrated subsistence system. Timely rain and appropriate temperature predominately affects crop distribution and yield. Climate change is evident in Nepal and it is imperative to understand how it affects habitat suitability of finger millet. Main objective of this study was to map the current suitable habitat and predicting the potential changes in the future under different climate scenarios in Nepal. Habitat mapping is important for maximizing production and minimizing the loss of local landraces. Results: Maxent model was used in this study to quantify the current suitable habitat and changes in the future habitat suitability of finger millet, based on representative concentration pathways (RCP)(RCP 2.6, 4.5, 6.0 and 8.5) in two different time periods (2050 and 2070AD) using climatic predictive variables and species localities. The model shows that 39.7% (58512.71km2) area of Nepal is highly suitable for finger millet, with cultivation mostly between 96-2300m above sea level. Eastern and central parts of Nepal have more suitable areas than western parts. Our research clearly shows that the future climatic suitable area of finger millet would shrink by 4.3 to 8.9% in 2050 and 8.9-10.5% under different RCPs by 2070. Conclusion: Finger millet is mostly cultivated in mid-hill terraces. The substantial increase in temperature due to climate change may be one reason for decrease in habitat suitability of finger millet. This situation would further threat loss of local landraces of finger millet in the future. The findings can help in planning and policy framing for climate resilient smart agriculture practice. Key words: Climate change, finger millet, habitat suitability, Maxent model

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“…However, even though traditional finger millet varieties are adapted to current environmental conditions, it is predicted that they will be less suitable to the changing climate. Research results indicate that finger millet is sensitive to high temperature stress during reproductive stages, and there is genotypic variability among finger millet genotypes for number of seeds per panicle and grain yield under high temperature stress, therefore the challenge will be to accelerate its adaptation to climate change (Vermeulen et al, 2012, Opole et al, 2018. Additionally, the projected food demand for 2025 will require the yield of millets to rise from 2.5 to 4.5 t ha -1 (Borlaug, 2002).…”

Section: Finger Millet Production and Utilization Potential In Africamentioning

confidence: 99%

Opportunities for Enhancing Production, Utilization and Marketing of Finger Millet in Africa

Opole¹

2019

AJFAND

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The world is faced with the challenge to produce more food to feed a projected 9 billion people worldwide; including 2.5 billion in Africa. Just a few "mega-crops" currently feed the world, with rice, wheat and maize providing 60 percent of the total population's energy intake. Finger millet belongs to a group of secondary crops that provide another 25 percent of the world's food energy. Finger millet is adaptable to diverse agro-ecological conditions, has beneficial nutritional properties and outstanding agronomic attributes as a subsistence food crop; therefore, it holds promise for the future of food and nutrition security in Africa and around the world. Despite these desirable attributes, only about 3 million tones are produced globally each year. Common production constraints include low soil fertility, Striga weed infestation, and pest and disease pressure, particularly finger millet blast disease, which can cause up to 50% yield loss. Low rates of finger millet production are compounded by a lack of stable market outlets and inadequate product development strategies. Recent research efforts in Africa and South Asia, however, have the potential of alleviating these production constraints. Opportunities now exist for enhanced technology development, which may increase production, product development, value addition, marketing and consumption of finger millet. Capitalizing on these opportunities could ensure that finger millet, as a "novel" crop, increases food and nutrition security in Africa and around the world.

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Thresholds, sensitive stages and genetic variability of finger millet to high temperature stress

Cited by 26 publications

References 62 publications

Seasonal Weather Changes Affect the Yield and Quality of Recombinant Proteins Produced in Transgenic Tobacco Plants in a Greenhouse Setting

Seasonal Weather Changes Affect the Yield and Quality of Recombinant Proteins Produced in Transgenic Tobacco Plants in a Greenhouse Setting

Potential suitable habitat of Eleusine coracana (L) Gaertn (Finger millet) under the climate change scenarios in Nepal

Opportunities for Enhancing Production, Utilization and Marketing of Finger Millet in Africa

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