Three β-tubulin cDNAs from the parasitic nematode Haemonchus contortus

Are tubulin isotypes functionally significant.

“…By this criterion, most of the isotypes listed in Table 1 cannot be dated. Most of the fungal and protist isotypes do not meet this criterion, nor do the d isotypes of the nematodes Haemonchus and Caenorhabditis, which suggests that these isotypes diverged at some time subsequent to the origin of the phyla Ascomycota or Nematoda (Little and Seehaus, 1988;Geary et al, 1992). Comparison of the sequences of the a isotypes of the higher plants suggests a more complex pattern, with the A3 isotypes of the pea (a dicot) meeting the criterion when compared with the ,B isotypes of corn (a monocot).…”

A possible model of benzimidazole binding to β-tubulin disclosed by invoking an inter-domain movement

“…Using the btubulin sequences of Fasciola hepatica [15] and H. contortus isotype b12-16 [16] model structures were generated from the b-subunit of the refined bovine ab-tubulin dimer atomic structure [17], which is available from the Brookhaven Data Bank (PDB Accession number, 1JFF). The alignment of these sequences is shown in Fig.…”

Individual Expression of Recombinant α- and β-Tubulin from Haemonchus contortus: Polymerization and Drug Effects

“…One ␣-and two ␤-tubulin cDNAs [␤8 -9 and ␤12-16, representing different H. contortus ␤-tubulin isoforms which have been previously described (10,19)] in pTrp2 were introduced into competent E. coli cells (strains Jm101 and Jm109) using heat shock and positive transformants selected on ampicillin plates. A single colony was then grown to mid-log phase in LB medium containing ampicillin and 1 mg/mL tryptophan, and the cells were harvested and grown for an additional 3-4 h in minimal medium (22) in the absence of tryptophan.…”