Three-week sprint interval training (SIT) reduces cell-free DNA and low-frequency fatigue but does not induce VO2max improvement in older men

Juškevičiūtė, Ema; Neuberger, Elmo; Eimantas, Nerijus; Venckunas, Tomas; Kamandulis, Sigitas; Simon, Perikles; Brazaitis, Marius

doi:10.1007/s00421-023-05366-2

Cited by 2 publications

(3 citation statements)

References 57 publications

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“…The underlying mechanisms of LFF differ depending on whether it is caused by metabolic or mechanical stress during exercise (Skurvydas et al., 2016 ). We recently reported a correlation between cfDNA levels and LFF during highly metabolically demanding exercise (Juškevičiūtė et al., 2023 ). The present study showed a stronger correlation between these two variables when only mechanical stress was applied, in comparison to our previous results on young subjects.…”

Section: Discussionmentioning

confidence: 97%

“…Only one study focused solely on eccentric training, where high‐intensity and low‐intensity eccentric cycling exercise was compared (Mavropalias et al., 2021 ). Although the protocol involved eccentric exercise, the participants performed high‐intensity cycling, in which the accumulation of metabolites would have been present but lower than that of exercise in most previous studies (Juškevičiūtė et al., 2023 ; Tug et al., 2017 ). Hence, the extent to which the increase in cfDNA is caused by mechanically demanding exercise alone is unclear.…”

Section: Introductionmentioning

confidence: 99%

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Cell‐free DNA kinetics in response to muscle‐damaging exercise: A drop jump study

Juškevičiūtė,

Neuberger,

Eimantas

et al. 2024

Experimental Physiology

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A significant increase in circulating cell‐free DNA (cfDNA) occurs with physical exercise, which depends on the type of exertion and the duration. The aims of this study were as follows: (1) to investigate the time course of cfDNA and conventional markers of muscle damage from immediately after to 96 h after muscle‐damaging exercise; and (2) to investigate the relationship between cfDNA and indicators of primary (low‐frequency fatigue and maximal voluntary isometric contraction) and secondary (creatine kinase and delayed‐onset muscle soreness) muscle damage in young healthy males. Fourteen participants (age, 22 ± 2 years; weight, 84.4 ± 11.2 kg; height, 184.0 ± 7.4 cm) performed 50 intermittent drop jumps at 20 s intervals. We measured cfDNA and creatine kinase concentrations, maximal voluntary isometric contraction torque, low‐frequency fatigue and delayed‐onset muscle soreness before and at several time points up to 96 h after exercise. Plasma cfDNA levels increased from immediately postexercise until 72 h postexercise (P < 0.01). Elevation of postexercise cfDNA was correlated with both more pronounced low‐frequency fatigue (r = −0.52, P = 3.4 × 10−11) and delayed‐onset muscle soreness (r = 0.32, P = 0.00019). Levels of cfDNA change in response to severe primary and secondary muscle damage after exercise. Levels of cfDNA exhibit a stronger correlation with variables related to primary muscle damage than to secondary muscle damage, suggesting that cfDNA is a more sensitive marker of acute loss of muscle function than of secondary inflammation or damaged muscle fibres.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Cell‐free DNA kinetics in response to muscle‐damaging exercise: A drop jump study

Juškevičiūtė,

Neuberger,

Eimantas

et al. 2024

Experimental Physiology

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“…In the first phase, participants visited the laboratory on two separate occasions, with a 2-day interval, for measurements of musculus vastus lateralis mitochondrial respiration using NIRS during repetitive occlusions following an electric muscle stimulation protocol. In the second phase, within one week, a cycling ramp test until exhaustion was conducted to measure whole-body aerobic power [29].…”

Section: Study Organizationmentioning

confidence: 99%

Near-InfraRed Spectroscopy Provides a Reproducible Estimate of Muscle Aerobic Capacity, but Not Whole-Body Aerobic Power

Venckunas,

Satas,

Brazaitis

et al. 2024

Sensors

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Near-infrared spectroscopy (NIRS) during repeated limb occlusions is a noninvasive tool for assessing muscle oxidative capacity. However, the method’s reliability and validity remain under investigation. This study aimed to determine the reliability of the NIRS-derived mitochondrial power of the musculus vastus lateralis and its correlation with whole-body (cycling) aerobic power (V̇O2 peak). Eleven healthy active men (28 ± 10 y) twice (2 days apart) underwent repeated arterial occlusions to induce changes in muscle oxygen delivery after 15 s of electrical muscle stimulation. The muscle oxygen consumption (mV̇O2) recovery time and rate (k) constants were calculated from the NIRS O2Hb signal. We assessed the reliability (coefficient of variation and intraclass coefficient of correlation [ICC]) and equivalency (t-test) between visits. The results showed high reproducibility for the mV̇O2 recovery time constant (ICC = 0.859) and moderate reproducibility for the k value (ICC = 0.674), with no significant differences between visits (p > 0.05). NIRS-derived k did not correlate with the V̇O2 peak relative to body mass (r = 0.441, p = 0.17) or the absolute V̇O2 peak (r = 0.366, p = 0.26). In conclusion, NIRS provides a reproducible estimate of muscle mitochondrial power, which, however, was not correlated with whole-body aerobic capacity in the current study, suggesting that even if somewhat overlapping, not the same set of factors underpin these distinct indices of aerobic capacity at the different (peripheral and whole-body systemic) levels.

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Three-week sprint interval training (SIT) reduces cell-free DNA and low-frequency fatigue but does not induce VO2max improvement in older men

Cited by 2 publications

References 57 publications

Cell‐free DNA kinetics in response to muscle‐damaging exercise: A drop jump study

Cell‐free DNA kinetics in response to muscle‐damaging exercise: A drop jump study

Near-InfraRed Spectroscopy Provides a Reproducible Estimate of Muscle Aerobic Capacity, but Not Whole-Body Aerobic Power

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