Three-step experimental design to determine the effect of process parameters on the size of chitosan microspheres

Biró, Emese; Németh, Ágnes Sz.; Feczkó, Tivadar; Tóth, Judit; Sisak, Cs.; Gyenis, János

doi:10.1016/j.cep.2008.10.001

Cited by 28 publications

(9 citation statements)

References 8 publications

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“…This process is made more challenging because of the complexity of confined polyelectrolyte (bio)polymer solutions including their rheological behavior and consequently their ability to produce defined solid particles especially in submicron size ranges. Biro et al investigated various parameters to control chitosan particle size distribution within stirred emulsions,22, 23 while others have produced chitosan microparticles and nanoparticles without focusing on the parameters affecting the particle size distribution.…”

Section: Introductionmentioning

confidence: 99%

Tailoring the surface of magnetic microparticles for protein immobilization

Reichelt

Elsner

Pender

et al. 2011

J of Applied Polymer Sci

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Magnetic, amine-functionalized microparticles (dynabeadsV R ) were surface-modified with different polyamines to suppress the nonspecific protein adsorption to a maximum extent but to allow for the enhanced covalent attachment of selected biomolecules (proteins). The stepwise chemical modification entailed the consecutive reaction of amine-functionalized dynabeadsV R with glutaric dialdehyde (GA) followed by the reaction with multifunctional, amine-containing compounds such as poly(allyl amine), poly(ethylene imine), and bovine serum albumin. The model proteins trypsin and concanavalin A, respectively, were finally covalently bound to the polyaminefunctionalized dynabeads via GA-mediated coupling. Matrix-assisted laser-desorption/ionization time of flight/ time of flight mass spectrometry confirmed the successful protein attachment. Both the nonspecific protein adsorption to the different polyamine-modified surfaces and the GA-mediated covalent binding of the target proteins to the polyamine-functionalized surfaces was quantified by standard bioassays. Compared to unmodified beads, the bioactivity of the polyamine-functionalized ones was increased by a factor of seven while keeping the nonspecific protein adsorption of a selected cationic protein at a very low level.

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Section: Introductionmentioning

confidence: 99%

Tailoring the surface of magnetic microparticles for protein immobilization

Reichelt

Elsner

Pender

et al. 2011

J of Applied Polymer Sci

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“…3b) make it potentially useful in immobilization of various enzymes (Fang et al, 2009). Results of several studies have been shown that after selection of suitable enzyme immobilization method, chitosan reactive amino and hydroxyl groups offer a good enzyme coupling efficiency (Luo et al, 2005;Biro et al, 2009;Yang et al, 2010).…”

Section: Immobolized Enzymes Onto Chitosanmentioning

confidence: 99%

“…Specific surface available to bind the applied enzyme and the contacting area with the substrate during a reaction mainly depends on particle size. Therefore, a lot of efforts were made to minimize the particle size and maximize the specific surface area (Biro et al, 2009). In addition, the physical characteristics of nanoparticles such as enhanced diffusion and particle mobility can impact inherent catalytic activity of attached enzymes (Jia et al, 2003).…”

Section: Ajbbmentioning

confidence: 99%

“…In this case, suitable methods are necessary to apply to facilitate the separation of the catalyst particles. Therefore, the general use of nanosized catalysts in industrial biotechnology needs considerable research efforts yet (Biro et al, 2009).…”

Section: Challenges In Applications Of Nanoparticles To Immobilize Enmentioning

confidence: 99%

“…However, more and more results are reported on immobilization of enzymes onto microparticles possessing high specific surface area and numerous active sites available for the enzyme molecules to be fixed. Moreover, because of the smaller size of the support particles, the internal diffusion hindrance diminishes (Biro et al, 2009). Hindrance can be described as a function of solute size and geometric properties of the porous network.…”

Section: Introductionmentioning

confidence: 99%

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Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Malmiri

Jahanian

Berenjian

2012

American Journal of Biochemistry and Biotechnology

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Chitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the area of preparation of immobilized enzyme carriers. This study looks at characteristics and applications of chitosan and chitosan nanoparticles and their potentials as suitable supports for enzyme immobilization. Results indicated that activity of immobilized enzymes and performance of enzyme immobilization onto chitosan nanoparticles are higher than chitosan macro and microparticles. As compared to other biopolymers nanoparticles, application of chitosan nanoparticles to immobilize enzymes strongly increases stability of immobilized enzymes and their easy separability from the reaction mixture at the end of the biochemical process.

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