Three-stage anaerobic co-digestion of food waste and horse manure

Abstract

A novel compact three-stage anaerobic digester (HM3) was developed to combine the advantages of high solids anaerobic digestion (AD) and wet AD for co-digestion of food waste and horse manure. By having three separate chambers in the three-stage anaerobic digester, three different functional zones were created for high-solids hydrolysis, acidogenesis and wet methanogenesis. The results showed that the functionalized partitioning in HM3 significantly accelerated the solubilization of solid organic matters and the formation of volatile fatty acids, resulting in an increase of 11~23% in methane yield. VS reduction in the HM3 presents the highest rate of 71% compared to the controls. Pyrosequencing analysis indicated that different microbial communities in terms of hydrolyzing bacteria, acidogenic bacteria and methanogenic archaea were selectively enriched in the three separate chambers of the HM3. Moreover, the abundance of the methanogenic archaea was increased by 0.8~1.28 times compared to controls.

A Review on Anaerobic Co-Digestion with a Focus on the Microbial Populations and the Effect of Multi-Stage Digester Configuration

“…However, they were enriched in the methanogenic stage. The abundance of the methanogenic archaea was increased by 0.8 and 1.28 times in the two-stage and the three-stage digesters compared to the single stage one, respectively [87].…”

“…Lactobacillaceae and Pseudomonadaceae were found to be the two predominant hydrolyzing bacteria in the hydrolysis digester. In comparison, the dominant species in acidogenic stage were Porphyromonadaceae and Enterobacteriaceae, corresponding to almost 70% of the total bacteria [87].…”

Design and construction of fixed dome digester for biogas production using cow dung and water hyacinth

“…Despite the diverse applicability and rapid expansion of biogas globally, some factors including process complexity, poor stability, inefficient biodegradability, substrate complexity, and low productivity impede methane production from AD. Numerous ways to overcome operational shortcomings suppressing methane yield have been suggested in previous studies, where the innovative approaches like three-stage digester (Zhang et al, 2017), novel enzyme addition (Dollhofer et al, 2015) and continuous microbial growth analysis (Sasidharan et al, 2018) have been developed and implemented successfully. In parallel, optimization of the process performance by manipulating operational variables (Hublin et al, 2012) such as feedstock choice, pretreatment, co-digestion, reactor type, temperature, pH and HRT (Hydraulic retention time) (Ward et al, 2008) have been widely considered.…”

A GIS‐based spatial index of feedstock‐mixture availability for anaerobic co‐digestion of Mediterranean by‐products and agricultural residues

“…Anaerobic co‐digestion of different organic residues has been widely investigated with a view to enhancing the performance of anaerobic digestion regarding biogas production and total solids reduction . Usually, during a co‐digestion process one main basic feedstock (e.g., animal manure or sewage sludge) is mixed with a smaller amount of a second one (e.g., crop residues, silage, and food wastes) to feed the digester . Only a few studies have examined the co‐digestion of multiple feedstocks (Valenti et al ., unpublished) and demonstrated successful biogas production from multiple organic residues.…”