Three specific antigens to isolate endothelial progenitor cells from human liposuction material

Hager, G.; Holnthoner, Wolfgang; Wolbank, Susanne; Husa, Anna-Maria; Godthardt, K.; Redl, Heinz; Gabriel, Christian

doi:10.1016/j.jcyt.2013.06.018

Cited by 26 publications

(13 citation statements)

References 44 publications

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“…We have defined hASCs according to these criteria [20][21][22]. Other methods have been frequently used to identify, isolate, and characterize human and murine ASCs from adipose tissue [23,38]. Thus, previous studies have reported that human and murine WAT is a rich reservoir of CD45 -CD34 + ASCs, with a high basal migration capacity in lean-derived, but not obese-derived, ASCs [23,39].…”

Section: Discussionmentioning

confidence: 99%

Obesity Determines the Immunophenotypic Profile and Functional Characteristics of Human Mesenchymal Stem Cells From Adipose Tissue

Pachón-Peña

Serena

Ejarque

et al. 2016

Stem Cells Translational Medicine

105

109

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Adipose tissue is a major source of mesenchymal stem cells (MSCs), which possess a variety of properties that make them ideal candidates for regenerative and immunomodulatory therapies. Here, we compared the immunophenotypic profile of human adipose-derived stem cells (hASCs) from lean and obese individuals, and explored its relationship with the apparent altered plasticity of hASCs. We also hypothesized that persistent hypoxia treatment of cultured hASCs may be necessary but not sufficient to drive significant changes in mature adipocytes. hASCs were obtained from subcutaneous adipose tissue of healthy, adult, female donors undergoing abdominal plastic surgery: lean (n = 8; body mass index [BMI]: 23 6 1 kg/m 2 ) and obese (n = 8; BMI: 35 6 5 kg/m 2 ). Cell surface marker expression, proliferation and migration capacity, and adipogenic differentiation potential of cultured hASCs at two different oxygen conditions were studied. Compared with lean-derived hASCs, obese-derived hASCs demonstrated increased proliferation and migration capacity but decreased lipid droplet accumulation, correlating with a higher expression of human leukocyte antigen (HLA)-II and cluster of differentiation (CD) 106 and lower expression of CD29. Of interest, adipogenic differentiation modified CD106, CD49b, HLA-ABC surface protein expression, which was dependent on the donor's BMI. Additionally, low oxygen tension increased proliferation and migration of lean but not obese hASCs, which correlated with an altered CD36 and CD49b immunophenotypic profile. In summary, the differences observed in proliferation, migration, and differentiation capacity in obese hASCs occurred in parallel with changes in cell surface markers, both under basal conditions and during differentiation. Therefore, obesity is an important determinant of stem cell function independent of oxygen tension. STEM CELLS TRANSLATIONAL MEDICINE 2016;5:464-475 SIGNIFICANCEThe obesity-related hypoxic environment may have latent effects on human adipose tissue-derived mesenchymal stem cells (hASCs) with potential consequences in mature cells. This study explores the immunophenotypic profile of hASCs obtained from lean and obese individuals and its potential relationship with the altered plasticity of hASCs observed in obesity. In this context, an altered pattern of cell surface marker expression in obese-derived hASCs in both undifferentiated and differentiated stages is demonstrated. Differences in proliferation, migration, and differentiation capacity of hASCs from obese adipose tissue correlated with alterations in cell surface expression. Remarkably, altered plasticity observed in obese-derived hASCs was maintained in the absence of hypoxia, suggesting that these cells might be obesity conditioned.

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Section: Discussionmentioning

confidence: 99%

Obesity Determines the Immunophenotypic Profile and Functional Characteristics of Human Mesenchymal Stem Cells From Adipose Tissue

Pachón-Peña

Serena

Ejarque

et al. 2016

Stem Cells Translational Medicine

105

109

View full text Add to dashboard Cite

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“…These EPCs formed capillary‐like structures in static Matrigel and acellular biological scaffold and secreted VEGF, supporting an angiogenic role for EPCs . Hager and colleagues combined CD31, CD144, VEGF‐R2, CD146, CD73, and CD105 to isolate rare human EPCs that could differentiate into endothelial cells in vitro . Perhaps more importantly, it is known that in vitro cultures of blood yield two distinct subpopulations of EPCs: early EPCs (eEPCs) and outgrowth endothelial cells (OECs) .…”

Section: Therapeutic Potential Of Svfmentioning

confidence: 96%

Concise Review: Fat and Furious: Harnessing the Full Potential of Adipose-Derived Stromal Vascular Fraction

Dykstra

Facile

Patrick

et al. 2017

Stem Cells Translational Medicine

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Due to their capacity to self‐renew, proliferate and generate multi‐lineage cells, adult‐derived stem cells offer great potential for use in regenerative therapies to stop and/or reverse degenerative diseases such as diabetes, heart failure, Alzheimer's disease and others. However, these subsets of cells can be isolated from different niches, each with differing potential for therapeutic applications. The stromal vascular fraction (SVF), a stem cell enriched and adipose‐derived cell population, has garnered interest as a therapeutic in regenerative medicine due to its ability to secrete paracrine factors that accelerate endogenous repair, ease of accessibility and lack of identified major adverse effects. Thus, one can easily understand the rush to employ adipose‐derived SVF to treat human disease. Perhaps faster than any other cell preparation, SVF is making its way to clinics worldwide, while critical preclinical research needed to establish SVF safety, efficacy and optimal, standardized clinical procedures are underway. Here, we will provide an overview of the current knowledge driving this phenomenon, its regulatory issues and existing studies, and propose potential unmapped applications. Stem Cells Translational Medicine 2017;6:1096–1108

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“…Выбор данных маркеров связан с тем, что на мембране циркулирующих в перифериче-ской крови EPC присутствует CD34, после адге-зии EPC и в процессе дифференцировки в зрелые эндотелиальные клетки экспрессия CD34 снижа-ется и исчезает, параллельно с этим процессом появляется и повышается экспрессия рецепто-ров CD31 [11]. В исследовании CD31 являлся маркером эндотелиальных клеток на внутренней стенке имплантированных графтов, сочетание CD31 с CD34 выявляло степень степени зрело-сти EPC, докраска ядер клеток DAPI позволяла исключить из анализа тромбоциты.…”

Section: рисунок 4 результаты гистологического исследования эксплантunclassified

Modification by RGD-Peptides of Vascular Grafts of Small Diameter From Polyparolactone: Experimental Study Results

Матвеева¹,

Антонова²,

Sevost'yanova³

et al. 2017

Kompleks. probl. serdečno-sosud. zabol.

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Three specific antigens to isolate endothelial progenitor cells from human liposuction material

Cited by 26 publications

References 44 publications

Obesity Determines the Immunophenotypic Profile and Functional Characteristics of Human Mesenchymal Stem Cells From Adipose Tissue

Obesity Determines the Immunophenotypic Profile and Functional Characteristics of Human Mesenchymal Stem Cells From Adipose Tissue

Concise Review: Fat and Furious: Harnessing the Full Potential of Adipose-Derived Stromal Vascular Fraction

Modification by RGD-Peptides of Vascular Grafts of Small Diameter From Polyparolactone: Experimental Study Results

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