Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis

Paule, Bruno Jean Adrien; Meyer, Roberto; Moura-Costa, Lília Ferreira de; Bahia, Robson; Carminati, Renato; Regis, Lia; Vale, Vera Lúcia Costa; Freire, Songelí Menezes; Nascimento, Ivana; Schaer, Robert; Azevedo, Vasco

doi:10.1016/j.pep.2003.12.003

Cited by 56 publications

(48 citation statements)

References 11 publications

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“…described, using immunoblot techniques [86,87], these proteins have not been identified. However, they reliably detected CLA infection in goats, and they could be used as vaccine components.…”

Section: Identification Of Immunodominant Peptidesmentioning

confidence: 99%

“…The genus Corynebacterium belongs to a suprageneric group of actinomycetes that also includes the genera Mycobacterium, Nocardia and Rhodococcus [46,87,100,102]. These gram-positive bacteria (Corynebacterium, Mycobacterium, Nocardia and Rhodococcus species), termed the CMN group, constitute a very heterogeneous group; however, most of the species share particular characteristics, such as: (i) a specific cell wall organization, mainly characterized by the presence of a huge polymer complex composed of peptidoglycan, arabinogalactan and mycolic acids [5, 26-28, 39, 45, 48] and (ii) high G+C content (47-74%) [39,40,43,80].…”

Section: Introductionmentioning

confidence: 99%

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Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

et al. 2006

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-Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis (CLA), a common disease in small ruminant populations throughout the world. Once established, this disease is difficult to eradicate because drug therapy is not effective and because the clinical detection of infected animals is of limited efficiency. We reviewed the microbiological, biochemical and taxonomic features of C. pseudotuberculosis, general aspects of infection, the main virulence determinants and currently available commercial vaccines. We also examined the current molecular strategies for the study of virulence in C. pseudotuberculosis, including the latest research on the identification of novel virulence factors and genes, which will help us to better understand the biology of this microorganism. This knowledge may also contribute to the development of improved CLA vaccines, including subunit and DNA-based types, as well as to improve the diagnosis, treatment and control of this disease.

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Section: Identification Of Immunodominant Peptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

et al. 2006

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“…A protein-rich middle layer is formed in addition to the two liquid phases when applying such a system to crude protein extracts. An increased activity of enzymes is often seen in this midlayer [11,13]. Pigments, lipids and enzyme inhibitors are concentrated in the upper phase, while polar components like saccharides are enriched in the lower aqueous phase [10,14].…”

Section: Introductionmentioning

confidence: 99%

Three-phase partitioning as a rapid and efficient method for purification of invertase from tomato

Özer

Akardere

Çelem

et al. 2010

Biochemical Engineering Journal

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“…The genus Corynebacterium belongs to the group of actinomycetes that includes the CMN group (Corynebacterium, Mycobacterium, Nocardia and Rhodococcus species) (Hard, 1969;Songer et al, 1988;Songer, 1997;Paule et al, 2004;Dorella et al, 2006a). These Grampositive bacteria constitute a very heterogeneous group that shares particular characteristics, such as a specific cell wall organization (Hard, 1975;Funke et al, 1995;Collins et al, 1982Collins et al, , 1998Connor et al, 2000;Bayan et al, 2003;Hall et al, 2003;Dorella et al, 2006a) and high G + C content (47-74%) (Garg et al, 1985;Goodfellow, 1989;Funke et al, 1995;Navas, 1996;Dorella et al, 2006a).…”

Section: Introductionmentioning

confidence: 99%

A description of genes of Corynebacterium pseudotuberculosis useful in diagnostics and vaccine applications

D’Afonseca

Moraes²,

Dorella³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Corynebacterium pseudotuberculosis, a Gram-positive intracellular pathogen, is the etiological agent of caseous lymphadenitis or CLA. This bacterium infects goats and sheep and causes great economic losses worldwide annually, mainly for goat producers. Despite its importance, CLA is still poorly characterized. However, with advances in the genomic field, many C. pseudotuberculosis genes have already been characterized, mainly those related to virulence such as phospholipase D. Here, we examined the use of the several available genes of C. pseudotuberculosis and reviewed their applications in vaccine construction, more efficient diagnostics for CLA, and control of this disease, among other applications.

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Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis

Cited by 56 publications

References 11 publications

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

Three-phase partitioning as a rapid and efficient method for purification of invertase from tomato

A description of genes of Corynebacterium pseudotuberculosis useful in diagnostics and vaccine applications

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