Three novel SNPs of the bovine Tf gene in Chinese native cattle and their associations with milk production traits

Zh, Ju; Ql, Li; Jm, Huang; Mh, Hou; Rl, Li; Li, Jian-Bin; Jf, Zhong; Cf, Wang

doi:10.4238/vol10-1gmr1038

Cited by 17 publications

(10 citation statements)

References 36 publications

(34 reference statements)

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“…The milk traits included 305-day milk yield, fat percentage, protein percentage, and somatic cell score (SCS), which were provided by the Dairy Cattle Research Center of Shandong Province, Academy of Agricultural Sciences Dairy Herd Improvement Laboratory using the milk composition analyzer (Foss MilkScan FT 6000). Genomic DNA was extracted from cattle blood using a phenol/chloroform solution according to the method used by Ju et al (2011). DNA concentration was spectrophotometrically estimated and diluted to 50 ng/mL.…”

Section: Animals and Dna Extractionmentioning

confidence: 99%

Functional Characterization of Genetic Polymorphisms Identified in the Promoter Region of the BovinePEPSGene

Zheng

Huang

et al. 2012

DNA and Cell Biology

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Peptidase S (PEPS) is a metallopeptidase that cleaves N-terminal residues from proteins and peptides. PEPS is used as a cell maintenance enzyme with critical roles in peptide turnover. The promoter region located upstream of the initiation site plays an important role in regulating gene expression. Polymorphism in the promoter region can alter gene expression and lead to biological changes. In the current study, polymorphisms in the promoter region of the PEPS gene were investigated. Polymerase chain reaction (PCR)-restriction fragment length polymorphism and DNA sequencing methods were used to screen sequence variations in the promoter region of DNA samples from 743 Chinese Holstein cattle. Two polymorphisms (g. -534 T > C and g. -2545 G > A) were identified and eight haplotypes were classified by haplotype analysis. The two genetic polymorphisms and haplotypes were associated with fat percentage and somatic cell score in Chinese Holstein cattle. The results of real-time PCR showed that cow kidneys exhibit the highest PEPS expression level. Moreover, bioinformatics analysis predicted that the single-nucleotide polymorphism g. -534 T > C is located in the core promoter region and in the transcription factor binding sites. The promoter activities of the polymorphism of -543 T > C were measured by luciferase assay in the human kidney epithelial cell line 293T. Transcriptional activity is significantly lower in cell lines transfected with the reporter construct containing 2.5 kb upstream fragments with -543 C than in those with wild-type -543 T. The results indicated that genetic variation at locus -543 influences PEPS promoter activity. The genetic variation in the promoter region of PEPS gene may regulate PEPS gene transcription and might have consequences at a regulatory level.

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Section: Animals and Dna Extractionmentioning

confidence: 99%

Functional Characterization of Genetic Polymorphisms Identified in the Promoter Region of the BovinePEPSGene

Zheng

Huang

et al. 2012

DNA and Cell Biology

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“…In cattle, TF has been shown to be highly polymorphic [ 57 ] and a study in Chinese native cattle indicated that TF polymorphisms are associated with protein yield, 305-day milk yield and mastitis susceptibility. In addition, they showed that TF mRNA expression was higher in mastitis affected in comparison with unaffected mammary tissue [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Adrenal cortex expression quantitative trait loci in a German Holstein × Charolais cross

et al. 2016

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BackgroundThe importance of the adrenal gland in regard to lactation and reproduction in cattle has been recognized early. Caused by interest in animal welfare and the impact of stress on economically important traits in farm animals the adrenal gland and its function within the stress response is of increasing interest. However, the molecular mechanisms and pathways involved in stress-related effects on economically important traits in farm animals are not fully understood. Gene expression is an important mechanism underlying complex traits, and genetic variants affecting the transcript abundance are thought to influence the manifestation of an expressed phenotype. We therefore investigated the genetic background of adrenocortical gene expression by applying an adaptive linear rank test to identify genome-wide expression quantitative trait loci (eQTL) for adrenal cortex transcripts in cattle.ResultsA total of 10,986 adrenal cortex transcripts and 37,204 single nucleotide polymorphisms (SNPs) were analysed in 145 F2 cows of a Charolais × German Holstein cross. We identified 505 SNPs that were associated with the abundance of 129 transcripts, comprising 482 cis effects and 17 trans effects. These SNPs were located on all chromosomes but X, 16, 24 and 28. Associated genes are mainly involved in molecular and cellular functions comprising free radical scavenging, cellular compromise, cell morphology and lipid metabolism, including genes such as CYP27A1 and LHCGR that have been shown to affect economically important traits in cattle.ConclusionsIn this study we showed that adrenocortical eQTL affect the expression of genes known to contribute to the phenotypic manifestation in cattle. Furthermore, some of the identified genes and related molecular pathways were previously shown to contribute to the phenotypic variation of behaviour, temperament and growth at the onset of puberty in the same population investigated here. We conclude that eQTL analysis appears to be a useful approach providing insight into the molecular and genetic background of complex traits in cattle and will help to understand molecular networks involved.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0442-x) contains supplementary material, which is available to authorized users.

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“…Chinese native cattle resources are abundant (22,23). These cattle have a unique origin system and excellent genetic resources and ecological adaptability (24,25). These native cattle breeds have many specific genes and potentials for meat production (26,27).…”

Section: Discussionmentioning

confidence: 99%

Longissimus Dorsi Muscle Transcriptomic Analysis of Simmental and Chinese Native Cattle Differing in Meat Quality

et al. 2020

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With the rapid development of economy, the demand for beef, with regard to quantity and quality, by consumers has been increasing in China. Chinese native cattle are characterized by their abundant genetic resources, unique origins, large breeding stocks, and robust environmental adaptability. Thus, to explore the genetic mechanisms on regulating meat quality in Chinese native cattle is of great importance to satisfy increased requirements for beef production. In this study, we investigated three breeds of cattle, namely Yunling, Wenshan, and Simmental, at the age of 12 months. Animals were classified into three groups (n = 5/breed). Growth traits including body weight and body size and plasma hormone levels were measured. Body weight of Wenshan cattle was significantly lower than that of Yunling and Simmental cattle (P < 0.05). Again, body size indexes, such as withers height, body slanting length, chest circumference, and hip and rump length, were significantly lower in Wenshan cattle than those in Yunling and Simmental cattle (P < 0.05). However, there were no significant differences in those indexes between Yunling and Simmental cattle (P > 0.05). Cattle were slaughtered at the age of 18 months and then meat color, pH, pressing losses, muscle tenderness, and cooking losses were measured at 0, 1, 2, 3, 5, and 7 days. Data revealed differences in meat quality among the three breeds analyzed. Based on transcriptomic sequencing and bioinformatic analysis, we observed 3,198 differentially expressed genes related to meat quality, of which 1,750 genes were upregulated. Moreover, we found two important signaling pathways closely linked to meat quality, namely adipocytokine signaling pathway [e.g., Leptin receptor (LEPR)] and protein processing in the endoplasmic reticulum [e.g., signal transducer and activator of transcription 3 (STAT3), heat shock protein (HSPA12A), and calpain 1 (CAPN1)]. The results of transcripts were further verified by qRT-PCR. Using correlation analysis between gene expression levels and shear force, we also identified two functional genes (e.g., HSPA12A and CAPN1) associated with meat quality. Overall, this study provides new sights into novel targets and underlying mechanisms to modulate meat quality in Chinese native cattle.

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Three novel SNPs of the bovine Tf gene in Chinese native cattle and their associations with milk production traits

Cited by 17 publications

References 36 publications

Functional Characterization of Genetic Polymorphisms Identified in the Promoter Region of the BovinePEPSGene

Functional Characterization of Genetic Polymorphisms Identified in the Promoter Region of the BovinePEPSGene

Adrenal cortex expression quantitative trait loci in a German Holstein × Charolais cross

Longissimus Dorsi Muscle Transcriptomic Analysis of Simmental and Chinese Native Cattle Differing in Meat Quality

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