Three novel RNA viruses in the spider mite Tetranychus urticae and their possible interactions with the host RNA interference response

Niu, Jinzhi; Zhang, Wei; Sun, Qin-Zhe; Wang, Jinjun

doi:10.1016/j.jip.2019.107228

Cited by 13 publications

(8 citation statements)

References 13 publications

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“…In recent years, several nege/kitavirids have been reported not only from blood-sucking insects, but also from agriculturally important plant-feeding arthropods [ 12 – 14 ]. Understanding the evolutionary relationships among these viruses is pivotal as the emergence of current kitaviruses may represent recent cross-kingdom jumps from arthropod-infecting ancestors followed by new host adaptation processes, as documented, among others, for plant rhabdoviruses [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses

Quito-Avila,

Reyes-Proaño,

Armijos-Capa

et al. 2024

PLoS ONE

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This study presents the complete genome sequence of a novel nege-like virus identified in whiteflies (Bemisia tabaci MEAM1), provisionally designated as whitefly negevirus 1 (WfNgV1). The virus possesses a single-stranded RNA genome comprising 11,848 nucleotides, organized into four open reading frames (ORFs). These ORFs encode the putative RNA-dependent-RNA-polymerase (RdRp, ORF 1), a glycoprotein (ORF 2), a structural protein with homology to those in the SP24 family, (ORF 3), and a protein of unknown function (ORF 4). Phylogenetic analysis focusing on RdRp and SP24 amino acid sequences revealed a close relationship between WfNgV1 and Bemisia tabaci negevirus 1, a negevirus sequence recently discovered in whiteflies from Israel. Both viruses form a clade sharing a most recent common ancestor with the proposed nelorpivirus and centivirus taxa. The putative glycoprotein from ORF 2 and SP24 (ORF 3) of WfNgV1 exhibit the characteristic topologies previously reported for negevirus counterparts. This marks the first reported negevirus-like sequence from whiteflies in the Americas.

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Section: Discussionmentioning

confidence: 99%

Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses

Quito-Avila,

Reyes-Proaño,

Armijos-Capa

et al. 2024

PLoS ONE

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“…On this basis, it is tempting to speculate that during adaptation to plants, the presumed ancestor of kitaviruses lost arthropod fitness as it gradually adapted to plant hosts, but still, some viral factors required for its interaction with the arthropod were retained, for instance, those minimal components allowing for the circulative route using paracellular spaces. The study of Tetranychus urticae kitavirus ( Niu et al, 2019 ), the closest kita-like virus infecting mites known, would likely add new elements to the mechanism underlying the movement of nege-kita-like viruses in their hosts.…”

Section: Discussionmentioning

confidence: 99%

Circulative Transmission of Cileviruses in Brevipalpus Mites May Involve the Paracellular Movement of Virions

et al. 2022

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Plant viruses transmitted by mites of the genus Brevipalpus are members of the genera Cilevirus, family Kitaviridae, or Dichorhavirus, family Rhabdoviridae. They produce non-systemic infections that typically display necrotic and/or chlorotic lesions around the inoculation loci. The cilevirus citrus leprosis virus C (CiLV-C) causes citrus leprosis, rated as one of the most destructive diseases affecting this crop in the Americas. CiLV-C is vectored in a persistent manner by the flat mite Brevipalpus yothersi. Upon the ingestion of viral particles with the content of the infected plant cell, virions must pass through the midgut epithelium and the anterior podocephalic gland of the mites. Following the duct from this gland, virions reach the salivary canal before their inoculation into a new plant cell through the stylet canal. It is still unclear whether CiLV-C multiplies in mite cells and what mechanisms contribute to its movement through mite tissues. In this study, based on direct observation of histological sections from viruliferous mites using the transmission electron microscope, we posit the hypothesis of the paracellular movement of CiLV-C in mites which may involve the manipulation of septate junctions. We detail the presence of viral particles aligned in the intercellular spaces between cells and the gastrovascular system of Brevipalpus mites. Accordingly, we propose putative genes that could control either active or passive paracellular circulation of viral particles inside the mites.

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“…If any, monopartite nege-like viruses infecting brevipalpus mites have not been detected yet. However, besides a number of negeviruses (Agboli et al, 2019), Saiwaicho virus (Medd et al, 2018), aphis glycine virus 3 (Feng et al, 2017), and Tetranychus urticae kitavirus (Niu et al, 2019), have been recovered from herbivore arthropods. In such a way, the role of arthropods acting as a bridge between ancestral nege-like viruses and plants seems to be probable.…”

Section: Discussionmentioning

confidence: 99%

Passion Fruit Green Spot Virus Genome Harbors a New Orphan ORF and Highlights the Flexibility of the 5′-End of the RNA2 Segment Across Cileviruses

et al. 2020

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Passion fruit green spot and passion fruit sudden death are two reportedly distinct viral diseases that recurrently affect passion fruit (Passiflora spp.) groves in Brazil. Here we used a systematic approach that interconnects symptoms, transmission electron microscopy, RT-PCR detection assays followed by Sanger sequencing, and highthroughput sequencing of the RNA of affected passion fruit plants to gain insights about these diseases. Our data confirmed not only the involvement of cileviruses in these two pathologies, as previously suggested, but also that these viruses belong to the same tentative species: passion fruit green spot virus (PfGSV). Results revealed that PfGSV has a positive-sense RNA genome split into two molecules of approximately 9 kb (RNA1) and 5 kb (RNA2), which share about 50-70% nucleotide sequence identity with other viruses in the genus Cilevirus. Genome sequences of five PfGSV isolates suggest that they have more conserved RNA1 (<5% of nucleotide sequence variability) compared to RNA2 (up to 7% of variability) molecules. The highest nucleotide sequence divergence among PfGSV isolates and other cileviruses is in the genomic segment covering from the 5-end of the RNA2 until the 5-end of the open reading frame (ORF) p61, which includes the ORF p15 and the intergenic region. This genomic stretch also harbors a novel orphan ORF encoding a 13 kDa protein presenting a cysteine-rich domain. High variability of 5end of the RNA2 in cileviruses is discussed in an evolutionary context assuming that they share putative common ancestors with unclassified arthropod-infecting single-strand positive RNA viruses, including mosquito-specific viruses of the group Negevirus (clades Nelorpivirus and Sandwavirus), and other viruses in the family Kitaviridae.

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Three novel RNA viruses in the spider mite Tetranychus urticae and their possible interactions with the host RNA interference response

Cited by 13 publications

References 13 publications

Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses

Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses

Circulative Transmission of Cileviruses in Brevipalpus Mites May Involve the Paracellular Movement of Virions

Passion Fruit Green Spot Virus Genome Harbors a New Orphan ORF and Highlights the Flexibility of the 5′-End of the RNA2 Segment Across Cileviruses

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