Three novel paralogs of the rodent prolactin gene family

Dai, Guoli; Wang, D; Liu, B; Kasik, JW; Müller, Heiner; White, R. A.; Hummel, GS; Soares, M.J.

doi:10.1677/joe.0.1660063

Cited by 28 publications

(22 citation statements)

References 59 publications

(69 reference statements)

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“…Cryosections (10 m) of tissues were prepared and stored at -80°C until used. Plasmids containing cDNAs for mouse Dprp and Plp-j (Orwig et al, 1997b;Dai et al, 2000) were used as templates to synthesize sense and antisense digoxigeninlabeled riboprobes according to the manufacturer's instructions (Roche Molecular Biochemicals, Indianapolis, IN).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…PRL is a member of a larger collection of structurally-related hormones/cytokines (the PRL superfamily) with an array of different biological targets and actions (Wiemers et al, 2003;Soares, 2004;Alam et al, 2006). In the rat and mouse, four members of the PRL superfamily are expressed in uterine decidua: decidual prolactin-related protein (DPRP; DTPRPMouse Genome Informatics) (Roby et al, 1993;Lin et al, 1997;Orwig et al, 1997b), prolactin-like protein B (PLP-B; PRLPBMouse Genome Informatics) (Duckworth et al, 1988;Croze et al, 1990;Cohick et al, 1997;Müller et al, 1998), PLP-J (PRLPJMouse Genome Informatics) (Hiraoka et al, 1999;Ishibashi and Imai, 1999;Toft and Linzer, 1999;Dai et al, 2000) and prolactin itself (Prigent-Tessier et al, 1999;Kimura et al, 2001). Each of these decidual PRL family cytokines can be viewed as a downstream mediator of intrauterine progesterone action.…”

Section: Introductionmentioning

confidence: 99%

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A uterine decidual cell cytokine ensures pregnancy-dependent adaptations to a physiological stressor

et al. 2007

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In the mouse, decidual cells differentiate from uterine stromal cells in response to steroid hormones and signals arising from the embryo. Decidual cells are crucially involved in creating the intrauterine environment conducive to embryonic development. Among their many functions is the production of cytokines related to prolactin (PRL), including decidual prolactin-related protein (DPRP). DPRP is a heparin-binding cytokine, which is abundantly expressed in uterine decidua. In this investigation, we have isolated the mouse Dprp gene, characterized its structure and evaluated its biological role. Dprp-null mice were made by replacing exons 2 to 6 of the Dprp gene with an in-frame enhanced green fluorescent protein (EGFP) gene and a neomycin (neo) resistance cassette. Heterozygous intercross breeding of the mutant mice yielded the expected mendelian ratio. Pregnant heterozygote females expressed EGFP within decidual tissue in locations identical to endogenous Dprp mRNA and protein expression. Homozygous Dprpnull mutant male and female mice were viable, exhibited normal postnatal growth rates, were fertile and produced normal litter sizes. A prominent phenotype was observed when pregnant Dprp-null mice were exposed to a physiological stressor. DPRP deficiency interfered with pregnancy-dependent adaptations to hypoxia resulting in pregnancy failure. Termination of pregnancy was associated with aberrations in mesometrial decidual cells, mesometrial vascular integrity, and disruptions in chorioallantoic placenta morphogenesis. The observations suggest that DPRP participates in pregnancy-dependent adaptations to a physiological stressor.

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Section: In Situ Hybridizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A uterine decidual cell cytokine ensures pregnancy-dependent adaptations to a physiological stressor

et al. 2007

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“…The trilaminar trophoblast includes a single layer of mononuclear sinusoidal trophoblast giant cells (S-TGCs) that line the maternal blood sinusoids (Coan et al, 2005;Simmons and Cross, 2005;Simmons et al, 2007), and two layers of syncytiotrophoblast, SynT-I and -II, the latter of which is in contact with fetal endothelial cells (Simmons and Cross, 2005;Watson and Cross, 2005). The STGCs are secretory in nature, expressing hormones such as placental lactogen II (Campbell et al, 1989;Dai et al, 2000;Deb et al, 1991;Ishida et al, 2004;Lee et al, 2003;Sahgal et al, 2000;Simmons and Cross, 2005;Simmons et al, 2007), and are therefore likely to have a primary endocrine function. S-TGCs are loosely attached to the underlying syncytial layers via desmosomal adhesions and contain fenestrations to allow the SynT-I cells direct access to maternal blood (Coan et al, 2005;Davies and Glasser, 1968;Hernandez-Verdun, 1974).…”

Section: Introductionmentioning

confidence: 99%

Early patterning of the chorion leads to the trilaminar trophoblast cell structure in the placental labyrinth

et al. 2008

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The labyrinth of the rodent placenta contains villi that are the site of nutrient exchange between mother and fetus. They are covered by three trophoblast cell types that separate the maternal blood sinusoids from fetal capillaries -a single mononuclear cell that is a subtype of trophoblast giant cell (sinusoidal or S-TGC) with endocrine function and two multinucleated syncytiotrophoblast layers, each resulting from cell-cell fusion, that function in nutrient transport. The developmental origins of these cell types have not previously been elucidated. We report here the discovery of cell-layer-restricted genes in the mid-gestation labyrinth (E12.5-14.5) including Ctsq in S-TGCs (also Hand1-positive), Syna in syncytiotrophoblast layer I (SynT-I), and Gcm1, Cebpa and Synb in syncytiotrophoblast layer II (SynT-II). These genes were also expressed in distinct layers in the chorion as early as E8.5, prior to villous formation. Specifically, Hand1 was expressed in apical cells lining maternal blood spaces (Ctsq is not expressed until E12.5), Syna in a layer immediately below, and Gcm1, Cebpa and Synb in basal cells in contact with the allantois. Cebpa and Synb were co-expressed with Gcm1 and were reduced in Gcm1 mutants. By contrast, Hand1 and Syna expression was unaltered in Gcm1 mutants, suggesting that Gcm1-positive cells are not required for the induction of the other chorion layers. These data indicate that the three differentiated trophoblast cell types in the labyrinth arise from distinct and autonomous precursors in the chorion that are patterned before morphogenesis begins.

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“…They demonstrated that PLP-J mRNA production is limited to early gestation, (peaking on day 7, and beginning to decrease on day 9, with undetectable levels by day 11) using the technique of in situ hybridization in placental tissue with a PLP-J probe. Another study demonstrated that the decidual expression of PLP-J is independent of extra-embryonic or embryonic factors (12).…”

Section: Introductionmentioning

confidence: 99%

Localization and expression patterns of prolactin-like protein J in mouse testis

Yang

Hao

et al. 2014

Molecular Medicine Reports

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Abstract. Prolactin (PRL)-like protein J (PLP-J) is a member of the prolactin family, mainly expressed in the placental decidua tissues of females, and is involved in gestation. To the best of our knowledge, it has not previously been shown to be expressed in males. Preliminary experiments of the present study indicated that PLP-J is expressed in the testis of male mice and is implicated in the regulation of testicular function. To definitively address whether PLP-J is expressed in the mouse testis, the expression pattern and cellular localization of PLP-J in mouse testes during postnatal development were characterized in the current study using molecular and immunological methods. Reverse transcription (RT)-polymerase chain reaction (PCR) was performed to amplify gene fragments from mouse testis specimens, which yielded sequences matching those of the PLP-J gene in Genbank. Subsequently, in situ hybridization showed that PLP-J was localized in interstitial tissue of the mouse testis. Immunofluorescence results indicated that PLP-J and 3β-hydroxysteroid dehydrogenase 1 were colocalized in testis Leydig cells, confirming PLP-J expression in Leydig cells. In addition, PLP-J gene expression levels were examined at different stages of postnatal mouse development in male testis tissues using quantitative RT-PCR and western blotting. The results revealed that PLP-J expression levels were lowest in 18-day-old mice and highest in adults aged 4 months. Levels observed in 16-month-old individuals were lower than those observed in the 4-month-old mice, but remained significantly higher than the levels observed in 18-day-old mice. Furthermore, the roles of PLP-J in the murine testis TM3 Leydig cell line were studied. The results demonstrated that the upregulation of PLP-J expression in TM3 Leydig cells did not affect testosterone production or the cell cycle. In conclusion, this study demonstrated that PLP-J, a known member of the PRL family that was previously considered to be expressed solely in females, is also expressed in the testis of males with an age-dependent expression profile. Nevertheless, the physiological role of PLP-J in males remains unclear.

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Three novel paralogs of the rodent prolactin gene family

Cited by 28 publications

References 59 publications

A uterine decidual cell cytokine ensures pregnancy-dependent adaptations to a physiological stressor

A uterine decidual cell cytokine ensures pregnancy-dependent adaptations to a physiological stressor

Early patterning of the chorion leads to the trilaminar trophoblast cell structure in the placental labyrinth

Localization and expression patterns of prolactin-like protein J in mouse testis

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