Three novel<i>Bacillus</i>strains from a traditional lacto-fermented pickle as potential probiotics

Talebi, Sara; Makhdoumi, Ali; Bahreini, Masoumeh; Matin, Maryam Moghaddam; Moradi, Hossein

doi:10.1111/jam.13901

Cited by 24 publications

(18 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Probiotics with antioxidant activity benefit the host by destroying and neutralizing free radicals ( Talebi et al 2018 ). At present, DPPH and ABTS + free radical scavenging activities are important tools to evaluate the antioxidant activity of probiotics.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro

Dai

Lin

et al. 2022

Polish Journal of Microbiology

View full text Add to dashboard Cite

The present study aimed to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from Chinese traditional fermented buffalo milk. Out of 22 isolates, 11 were putatively identified as LAB preliminarily. A total of six LAB strains displayed strong adhesion to HT-29 cells and all these strains showed preferable tolerance to artificially simulated gastrointestinal juices. WDS-4, WDS-7, and WDS-18 exhibited excellent antioxidant capacities, including DPPH radical, ABTS+ radical, and superoxide anion scavenging activities. Compared with the other two LAB strains, WDS-7 had a stronger inhibition effect on four pathogens. Based on the 16S rRNA gene sequencing and phylogenetic analysis, WDS-7 was identified as Lactobacillus delbrueckii ssp. indicus and selected to assess the potential and safety of probiotics further. The results revealed that WDS-7 strain had a strong capacity for acid production and good thermal stability. WDS-7 strain also possessed bile salt hydrolase (BSH) activity. Compared to LGG, WDS-7 was a greater biofilm producer on the plastic surface and exhibited a better EPS production ability (1.94 mg/ml as a glucose equivalent). WDS-7 was proved to be sensitive in the majority of tested antibiotics and absence of hemolytic activity. Moreover, no production of biogenic amines and β-glucuronidase was observed in WDS-7. The findings of this work indicated that L. delbrueckii ssp. indicus WDS-7 fulfilled the probiotic criteria in vitro and could be exploited for further evaluation in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro

Dai

Lin

et al. 2022

Polish Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…These ndings differ from our results. Three Bacillus were susceptible to ampicillin, erythromycin, and gentamicin [41]. Patel et al [42] reported that Bacillus isolates were highly susceptible to cipro oxacin.…”

Section: Discussionmentioning

confidence: 99%

Lysinibacillus capsici 38328 Isolated from Agricultural Soils as a Promising Probiotic Candidate for Intestinal Health

Chen

Sun

et al. 2022

Preprint

View full text Add to dashboard Cite

There is an increasing interest in the use of spore-forming Bacillus spp. as probiotic ingredients on the market. However, probiotics Bacillus species are insufficient, and more safe Bacillus species were required. In the study, traditional fermented foods and soil samples were collected from all over the country in China, and 506 Bacillus were selected from 109 samples. Using the optimized procedure, we screened 9 strains, which successfully passed the acid, alkali, bile salt and trypsin resistance test. Drug sensitivity test results showed that three Bacillus out of the 9 isolates exhibited antibiotic sensitivity to more than 29 antibiotics. The 3 strains sensitive to antibiotics were identified by 16S ribosomal RNA, recA and gyrB gene analysis, two isolates (38327 and 38328) belong to the specie Lysinibacillus capsici and one isolate (37326) belong to Bacillus halotolerans. Moreover, the 3 strains were confirmed safe through animal experiments. Finally, L. capsici 38327 and 38328 showed the protection in Salmonella infection mouse model, which manifested in slowing down weight loss, reducing bacterial load, improving antioxidant capacity. Altogether, our data demonstrate selected L. capsici strains can be used novel probiotics for intestinal health.

show abstract

“…coli strains are considered as harmless commensal bacteria; however, several strains have gained the ability to cause infections in human hosts. These pathogenic variants have obtained a variety of virulence factors that confer environmental adaptations and pathogenicity [13][14]. Moreover, these pathogenic variants have adopted several mechanisms to resist antibiotic therapy with the production of beta-lactamase enzymes being the most common strategy [15].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of the Spore-forming Bacillus spp. on the Expression Levels of eae, luxS, flu, and ctxM Genes in E. coli Isolates

Elahianfiroz

Baserisalehi

Ghane

2020

Preprint

View full text Add to dashboard Cite

Abstract Background: Escherichia coli antibiotic resistance are one of the major health problems in many countries. Today, researchers are focusing on novel approaches for the treatment of E. coli infections, including the use of spore-forming probiotics for their high stability in the harsh gastrointestinal (GIT) environment. Results: The presence of resistance genes (flu, eae, luxS, and ctxM ) was confirmed by PCR in four out of 40 E. coli isolated from 300 fecal samples of patients suffering from diarrhea. Following confirming the ability of broiler-derived Bacillus coagulans and Bacillus subtilis to survive under the simulant gastric environment, they were co-cultured with the four E. coli isolates harboring the studied resistance genes. Results of the expression of flu, luxS, eae, and ctxM genes revealed that the expression levels of these genes decreased significantly (p-value < 0.05) after co-culture of E. coli with B. subtilis ATCC 6633 and broiler-derived B. subtilis. However, the broiler-derived B. subtilis showed a greater effect on the expression levels of eae, luxS, and ctxM. B. coagulans MTCC 5856 and broiler-derived B. coagulans also led to significant decreases in expression levels of all the studied genes (p-value < 0.05), while the standard strain had a greater impact on the expression of ctxM than the broiler-derived strains.Conclusion: The broiler-derived isolates had a greater capacity to decrease the expression of these genes than the standard strains, proposing their adoption for dietary supplementations. However, further studies are required to study the effects of these spore-forming bacteria on E. coli infections in vivo and their possible influence on lactose tolerance, nutritional absorption, and reduced cholesterol levels.

show abstract

Three novelBacillusstrains from a traditional lacto-fermented pickle as potential probiotics

Abstract: Nondairy-fermented foods are interesting sources for isolation of novel probiotics. Identification of novel Bacillus strains with remarkable probiotic potentials would increase their contribution in food/feed and pharmaceutical industries.

Cited by 24 publications

References 38 publications

Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro

Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro

Lysinibacillus capsici 38328 Isolated from Agricultural Soils as a Promising Probiotic Candidate for Intestinal Health

Inhibitory Effects of the Spore-forming Bacillus spp. on the Expression Levels of eae, luxS, flu, and ctxM Genes in E. coli Isolates

Contact Info

Product

Resources

About