Three novel Actinoplanes species isolated by using polyaspartic acid as a water-retaining agent for the enrichment in situ

Abstract

Three novel actinomycete strains, designated TRM66264-DLMT, TRM88002T and TRM88003T, were isolated by using polyaspartic acid as a water-retaining agent for the enrichment in situ. The 16S rRNA gene sequence and phylogenetic analyses of three strains indicated that they belonged to the genus Actinoplanes . The phylogenetically closest strains of TRM66264-DLMT, TRM88002T and TRM88003T were Actinoplanes bogorensis LIPI11-2-Ac043T (98.4 %), Actinoplanes abujensis A4029T (98.0 %) and Actinoplanes ferrugineus IFO15555T (98.1 %), respectively. The major polar lipids of strains TRM66264-DLMT and TRM88002T were phosphatidylethanolamine and disphosphatidylglycerol, while strain TRM88003T only had phosphatidylethanolamine. The predominant menaquinones of strain TRM66264-DLMT were identified as MK-9(H4) and MK-9 (H6). Strains TRM88002T and TRM88003T had MK-9(H4). The cell-wall peptidoglycan of three strains contained meso-diaminopimelic acid. The whole-cell sugars of strain TRM66264-DLMT were identified as arabinose, glucose, galactose and xylose. Strains TRM88002T and TRM88003T mainly had arabinose and glucose. The DNA G+C content of strains TRM66264-DLMT, TRM88002T and TRM88003T were 70.48, 70.46 and 70.64 mol%, respectively. Genotypic and phenotypic analysis confirmed that all three strains sre new members of the genus Acinoplanes. Therefore, it is proposed that strains TRM66264-DLMT, TRM88002T and TRM88003T represent three novel species of the genus Actinoplanes , for which the names Actinoplanes polyasparticus sp. nov. (type strain TRM66264-DLMT=CCTCC AA 2021015T=LMG 32389T), Actinoplanes hotanensis sp. nov. (type strain TRM88002T=CCTCC AA 2021036T=LMG 32621T) and Actinoplanes aksuensis sp. nov. (type strain TRM88003T=CCTCC AA 2021037 T=LMG 32622T) are proposed.

Phylogenomic analyses of the genus Actinoplanes: description of four novel genera

“…The description is the same as for A. polyasparticus [51]. Phylogenetic and chemotaxonomic analysis strongly supported the placement of this species in the genus Paractinoplanes .…”

Actinoplanes pyxinae sp. nov., a new lichen-derived rare actinobacterium exhibiting antimicrobial and anticancer activity

“…Differential characteristics of strain Pm04-4 T and closely related Actinoplanes type strains Strains: 1, Pm04-4 T ; 2, A. aksuensis TRM88003 T ; 3, A. maris M416 T ; 4, A. polyasparticus TRM66264-DLM T ; 5, A. hotanensis TRM88002 T ; 6, A. lichenicola LDG1-01 T ; 7, A. brasiliensis DSM 43805 T ; 8, A. abujensis DSM 45518 T ; 9, A. bogorensis NBRC 110975 T ; 10, A. ovalisporus LDG1-06 T . All data obtained from this study, except those marked * and †, which were obtained from Ding et al [39] and Xie et al [40], respectively. +, Positive; w, weakly positive; −, negative; nd, no data.…”

Actinoplanes oblitus sp. nov., producing the glycopeptide antibiotic A477

“…Tetra-hydrogenated menaquinone with nine isoprene units – MK-9(H 4 ) – is commonly the predominant isoprenologue followed by minor amounts of hexa- and octahydrogenated menaquinones with nine isoprene units isoprenologues – MK-9(H 6 ) and MK-9(H 8 ) [2]. Iso/anteiso branched and monounsaturated fatty acids and/or cis -9,10-octadecanoic acid are often present, though their proportions may vary considerably between the species [2, 7–10, 12, 13].…”

“…At the time of writing (September 2023), the genus Actinoplanes consisted of 53 species with validly published species names, as reported in the List of Prokaryotic names with Standing in Nomenclature (https://lpsn.dsmz.de/genus/actinoplanes), including recently published Actinoplanes ramoplaninifer [7], Actinoplanes deserti [8], Actinoplanes flavus [9], Actinoplanes aksuensis , Actinoplanes polyasparticus , Actinoplanes hotanensis [10], and Actinoplanes maris [14]. Actinoplanes inhabit various natural environments and were isolated from temperate soils [15], mucky soils [12], arid and desert soils [8, 16], marine sediments [17], and rhizospheres of different plants [9, 18] or lichens [19].…”

“…The cell-wall composition of Actinoplanes is characterized by the presence of meso-2,6-diaminopimelic acid, which might be replaced with 2,6-diamino-3-hydroxypimelic acid [2,6]. The diagnostic sugars of whole-cell hydrolysates might vary in different Actinoplanes and may include glucose, xylose, galactose, arabinose, ribose, and mannose [7][8][9][10]. Phospholipid composition of Actinoplanes belongs to chemotaxonomic type PII (after Lechevalier et al [11]), being characterized by the presence of phosphatidylethanolamine and diphosphatidylglycerol as diagnostic phospholipids and traces of phosphatidylglycerol.…”