Three new species of Bullera isolated from leaves in the Ogasawara Islands

Abstract

Thirteen strains of ballistoconidium-forming yeasts were isolated from leaves collected in the Ogasawara Islands, Japan. They represent three different species in the genus Bullera on the basis of morphological, physiological, and biochemical characteristics, analyses of the sequences of internal transcribed spacer regions and small subunit ribosomal DNA, and a nuclear DNA-DNA hybridization study. Three new species, Bullera boninensis (five strains), B. waltii (seven strains), and B. schimicola (one strain), are proposed for these 13 strains.

Bullera cylindrica sp. nov., Bullera hubeiensis sp. nov. and Bullera nakasei sp. nov., ballistoconidium-forming yeast species from plant leaves

“…Three additional Bullera species isolated from the Ogasawara Islands, Japan, were added to the clade by Sugita et al (1999). The number of species in the clade was increased to eight by Bai et al (2001), who identified three novel Bullera species among strains originally assigned to Bullera variabilis and assigned these species to the B. mrakii clade by 18S rRNA gene and internal transcribed spacer (ITS) region sequence analyses.…”

Intragenomic polymorphism and intergenomic recombination in the ribosomal RNA genes of strains belonging to a yeast speciesPichia membranifaciens

“…Previous studies have claimed that conspecific yeast strains usually have fewer than 1% nucleotide differences in the ITS 1 and 2 regions overall (Nagahama et al 1999; Sugita et al 1999a, b; Scorzetti et al 2002). However, the present study showed that divergent ITS1 and ITS2 types which differed by up to 15.6% and 20.5% base mismatches, respectively, coexist in the single genome of strain CBS 215.…”

“…The rDNA ITS region is one of the most frequently used molecular markers in phylogeny and identification of yeasts at the species level (James et al 1996; Montrocher et al 1998; Sugita et al 1999a, b; Fell et al 2000; Scorzetti et al 2002; Kurtzman and Robnett 2003) and has been selected as a universal DNA barcode marker for Fungi (Schoch et al 2012). Previous studies have claimed that conspecific yeast strains usually have fewer than 1% nucleotide differences in the ITS 1 and 2 regions overall (Nagahama et al 1999; Sugita et al 1999a, b; Scorzetti et al 2002).…”

Phylogenetic analysis of strains originally assigned to Bullera variabilis: descriptions of Bullera pseudohuiaensis sp. nov., Bullera komagatae sp. nov. and Bullera pseudoschimicola sp. nov.

“…The B. variabilis strains in group III were located in a clade containing only one recently described species, B. schimicola (Sugita et al, 1999a) (Fig. 3).…”

“…Sequences of the 18S rDNA and ITS regions determined in this study and reference sequences were aligned with the   program (Thompson et al, 1994) and adjusted manually. The reference sequences were obtained from DDBJ, EMBL and GenBank, where they had been deposited by other authors (Sugita et al, 1999a ;Suh & Sugiyama, 1993 ;Suh et al, 1996a, b ;Swann & Taylor, 1993 ;Takashima & Nakase, 1998, 1999Van de Peer et al, 1992). The phylogenetic tree was constructed from the evolutionary distance data calculated from Kimura's two-parameter model (Kimura, 1980) using the neighbour-joining method (Saitou & Nei, 1987).…”