Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish

Kim, Hyoung‐Geun; Oh, Seon-Min; Kim, Na Woo; Shim, Ji Heon; Nam, Youn Hee; Nguyen, Trong Nguyen; Lee, Min-ho; Lee, Dong Hoon; Kang, Tong Ho; Baek, Nam‐In

doi:10.3390/molecules26227034

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…Neomycin is known to cause hair cell death in zebrafish [ 55 , 56 , 57 , 58 ], much like it does in HEI-OC1 cells. Various natural products have been reported to increase the number of hair cells in neomycin-damaged zebrafish larvae [ 59 , 60 ]. Therefore, the effect of TS treatment on the recovery of neomycin-induced hair cell loss was evaluated in zebrafish larvae.…”

Section: Discussionmentioning

confidence: 99%

Amelioration of Sensorineural Hearing Loss through Regulation of Trpv1, Cacna1h, and Ngf Gene Expression by a Combination of Cuscutae Semen and Rehmanniae Radix Preparata

et al. 2023

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Sensorineural hearing loss (SNHL) is a common condition that results from the loss of function of hair cells, which are responsible for converting sound into electrical signals within the cochlea and auditory nerve. Despite the prevalence of SNHL, a universally effective treatment has yet to be approved. To address this absence, the present study aimed to investigate the potential therapeutic effects of TS, a combination of Cuscutae Semen and Rehmanniae Radix Preparata. To this end, both in vitro and in vivo experiments were performed to evaluate the efficacy of TS with respect to SNHL. The results showed that TS was able to protect against ototoxic neomycin-induced damage in both HEI-OC1 cells and otic hair cells in zebrafish. Furthermore, in images obtained using scanning electron microscopy (SEM), an increase in the number of kinocilia, which was prompted by the TS treatment, was observed in the zebrafish larvae. In a noise-induced hearing loss (NIHL) mouse model, TS improved hearing thresholds as determined by the auditory brainstem response (ABR) test. Additionally, TS was found to regulate several genes related to hearing loss, including Trpv1, Cacna1h, and Ngf, as determined by quantitative real-time polymerase chain reaction (RT-PCR) analysis. In conclusion, the findings of this study suggest that TS holds promise as a potential treatment for sensorineural hearing loss. Further research is necessary to confirm these results and evaluate the safety and efficacy of TS in a clinical setting.

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Section: Discussionmentioning

confidence: 99%

Amelioration of Sensorineural Hearing Loss through Regulation of Trpv1, Cacna1h, and Ngf Gene Expression by a Combination of Cuscutae Semen and Rehmanniae Radix Preparata

et al. 2023

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“…, naringin (4), hesperidin (5), neohesperidin (6), liquiritigenin (7), glycyrrhizin (8), and 6-shogaol (9).…”

Section: Optimization Of Lc-ms/ms Mrm Conditionsmentioning

confidence: 99%

“…Nine herbal medicines have been reported to have various biological effects, for example, anti-inflammatory, antioxidant, anticancer, antitumor, antidiabetes, antiaging, anti-obesity, neuroprotective, and antibacterial activities [6][7][8][9][10][11][12][13][14]. Recently, a study on the safety of a single administration of GHT was reported by An et al [4], but few studies on the biological activity have been reported.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis for Quality Control of Traditional Herbal Medicine, Gungha-Tang, Using Liquid Chromatography–Tandem Mass Spectrometry

Seo

Shin

2022

Molecules

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Gungha-tang (GHT), a traditional herbal medicine, consists of nine medicinal herbs (Cnidii Rhizoma, Pinelliae Tuber, Poria Sclerotium, Citri Unshius Pericarpium, Citri Unshius Pericarpium Immaturus, Aurantii Fructus Immaturus, Atracylodis Rhizoma Alba, Glycyrrhizae Radix et Rhizoma, and Zingiberis Rhizoma Recens). It has been used for various diseases caused by phlegm. This study aimed to develop and verify the simultaneous liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis method, using nine marker components (liquiritin apioside, neoeriocitrin, narirutin, naringin, hesperidin, neohesperidin, liquiritigenin, glycyrrhizin, and 6-shogaol) for quality control of GHT. LC–MS/MS analysis was conducted using a Waters TQ-XS system. All marker analytes were separated on a Waters Acquity UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) using gradient elution with a distilled water solution (containing 5 mM ammonium formate and 0.1% [v/v] formic acid)–acetonitrile mobile phase. LC–MS/MS multiple reaction monitoring (MRM) analysis was carried out in negative and positive ion modes of an electrospray ionization source. The developed LC–MS/MS MRM method was validated by examining the linearity, limits of detection and quantification, recovery, and precision. LOD and LOQ values of nine markers were calculated as 0.02–8.33 ng/mL and 0.05–25.00 ng/mL. The recovery was determined to be 89.00–118.08% and precision was assessed with a coefficient of variation value of 1.74–8.64%. In the established LC–MS/MS MRM method, all markers in GHT samples were detected at 0.003–16.157 mg/g. Information gathered during the development and verification of the LC–MS/MS method will be useful for the quality assessment of GHT and other herbal medicines.

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“…The structural characterizations of phthalides are accomplished frequently by the analysis of Infrared (IR) spectroscopy, mass spectrometry (MS), GC coupled to selective mass detectors (GC‐MS) and high‐resolution mass spectrometry (HR‐MS), high‐performance liquid chromatography (HPLC), and 1D and 2D Nuclear Magnetic Resonance (NMR), electronic circular dichroism (ECD) and X‐ray crystallographic data, or frequently a combination of them has been applied [80–87] …”

Section: Introductionmentioning

confidence: 99%

An Overview of Natural and Synthetic Phthalides Involved in Cancer Studies: Past, Present, and Future

et al. 2022

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Phthalides, mainly isolated from two genera: Ligusticum and Angelica of the Apiceae (syn Umbelliferae) family, are thought to be a potent natural compound with many therapeutic effects, such as antiplatelet, antitussive, antinociceptive, anti‐inflammatory, antibacterial, antiviral, and immunosuppressive activities. They also have antifungal, antioxidant, and anticancer activities. Phthalides are responsible for the characteristic odor of celery. Since ancient times, phthalides containing plants have been used in Asia, Europe, and North America as traditional medicines and food flavoring agents. In this review, we have provided the data regarding the mechanisms of action related with phthalides on different cancer cells, their chemopreventive properties, and their possible therapeutic potential against various types of human cancer. Our attempt is to make a case for the use of phthalide and their derivatives as one of the plant‐based compounds in targeting metabolic treatment of cancer diseases.

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Three New Phthalide Glycosides from the Rhizomes of Cnidium officinale and Their Recovery Effect on Damaged Otic Hair Cells in Zebrafish

Cited by 7 publications

References 12 publications

Amelioration of Sensorineural Hearing Loss through Regulation of Trpv1, Cacna1h, and Ngf Gene Expression by a Combination of Cuscutae Semen and Rehmanniae Radix Preparata

Amelioration of Sensorineural Hearing Loss through Regulation of Trpv1, Cacna1h, and Ngf Gene Expression by a Combination of Cuscutae Semen and Rehmanniae Radix Preparata

Simultaneous Analysis for Quality Control of Traditional Herbal Medicine, Gungha-Tang, Using Liquid Chromatography–Tandem Mass Spectrometry

An Overview of Natural and Synthetic Phthalides Involved in Cancer Studies: Past, Present, and Future

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