Three new
            <i>Drosophila</i>
            markers of intracellular membranes

LaJeunesse, Dennis; Buckner, Stephanie M; Lake, Jeffrey P.; Na, Charles; Pirt, Amanda; Fromson, Kathryn

doi:10.2144/04365st01

Cited by 59 publications

(71 citation statements)

References 10 publications

(5 reference statements)

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“…3). Simultaneous immunohistological stainings of Drosophila tissue with anti-Garz antibodies and for Golgi-EYFP (LaJeunesse et al, 2004) revealed that Garz is highly enriched in the ultra-proximal region of the Golgi complex, which corresponds to the cis-Golgi compartment ( Fig. 3A-I).…”

Section: Ems667mentioning

confidence: 99%

GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis

Wang

Meyer

Ochoa-Espinosa

et al. 2012

Journal of Cell Science

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SummaryHere we report on the generation and in vivo analysis of a series of loss-of-function mutants for the Drosophila ArfGEF, Gartenzwerg. The Drosophila gene gartenzwerg (garz) encodes the orthologue of mammalian GBF1. garz is expressed ubiquitously in embryos with substantially higher abundance in cells forming diverse tubular structures such as salivary glands, trachea, proventriculus or hindgut. In the absence of functional Garz protein, the integrity of the Golgi complex is impaired. As a result, both vesicle transport of cargo proteins and directed apical membrane delivery are severely disrupted. Dysfunction of the Arf1-COPI machinery caused by a loss of Garz leads to perturbations in establishing a polarized epithelial architecture of tubular organs. Furthermore, insufficient apical transport of proteins and other membrane components causes incomplete luminal diameter expansion and deficiencies in extracellular matrix assembly. The fact that homologues of Garz are present in every annotated metazoan genome indicates that secretion processes mediated by the GBF-type ArfGEFs play a universal role in animal development.

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Section: Ems667mentioning

confidence: 99%

GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis

Wang

Meyer

Ochoa-Espinosa

et al. 2012

Journal of Cell Science

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“…Instead, the Drosophila Golgi exists as distributed puncta, forming multiple organelles within a single cell (Kondylis and Rabouille, 2009; Stanley et al, 1997). As the major change in sff N-glycan expression is a shift towards greater complexity, we focused first on late Golgi compartments using markers described previously to delineate the medial/trans and trans-Golgi in Drosophila (LaJeunesse et al, 2004;Yano et al, 2005). We assessed the relative distributions of Golgi-YFP (a medial/trans marker, YFP fused to the transmembrane localization domain of human 4GalT), Arachis hypogaea lectin (PNA) binding (a trans marker, Gal3GalNAc specific lectin) and a neuronal protein (Fasciclin 2, Fas2) that bears an HRP epitope (Desai et al, 1994;Lin et al, 1994).…”

Section: Sff Modulates Golgi Compartmentation In Neuronsmentioning

confidence: 99%

Sugar-free frosting, a homolog of SAD kinase, drives neural-specific glycan expression in the Drosophila embryo

et al. 2011

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SUMMARYPrecise glycan structures on specific glycoproteins impart functionalities essential for neural development. However, mechanisms controlling embryonic neural-specific glycosylation are unknown. A genetic screen for relevant mutations in Drosophila generated the sugar-free frosting (sff) mutant that reveals a new function for protein kinases in regulating substrate flux through specific Golgi processing pathways. Sff is the Drosophila homolog of SAD kinase, which regulates synaptic vesicle tethering and neuronal polarity in nematodes and vertebrates. Our Drosophila sff mutant phenotype has features in common with SAD kinase mutant phenotypes in these other organisms, but we detect altered neural glycosylation well before the initiation of embryonic synaptogenesis. Characterization of Golgi compartmentation markers indicates altered colocalization that is consistent with the detected shift in glycan complexity in sff mutant embryos. Therefore, in analogy to synaptic vesicle tethering, we propose that Sff regulates vesicle tethering at Golgi membranes in the developing Drosophila embryo. Furthermore, neuronal sff expression is dependent on transcellular signaling through a non-neural toll-like receptor, linking neural-specific glycan expression to a kinase activity that is induced in response to environmental cues.

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“…4, 5). Transgenic flies targeting expression of an enhanced green fluorescent protein (GFP) variant to either the ER or trans-Golgi (LaJeunesse et al, 2004) were analyzed by immunostaining with anti-GFP (see the Experimental Procedures section). We also used an anti-KDEL antibody to label the ER or an anti-KDEL receptor (anti-KDEL-R) antibody to visualize the ER-Golgi intermediate compartment and cis-Golgi (Tang et al, 1993;Griffiths et al, 1994).…”

Section: Loj Localizes To Punctate Structures In the Cytoplasm Of Devmentioning

confidence: 99%

“…Purified IgG made against GST-Loj was used at a concentration of 7 g/ml with ovaries dissected from CS, EYFP-ER (LaJeunesse et al, 2004), EYFP-Golgi (LaJeunesse et al, 2004), or loj 00898 (Carney and Taylor, 2003) flies. EYFP-ER and EYFP-Golgi tissues were also incubated with a 1:50 dilution of mouse monoclonal antibody 3E6 anti-GFP (Molecular Probes).…”

Section: Immunofluorescence Microscopymentioning

confidence: 99%

Drosophila melanogasterp24 genes have developmental, tissue‐specific, and sex‐specific expression patterns and functions

Boltz

Ellis

Carney

2006

Developmental Dynamics

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Genes encoding members of the p24 family of intracellular trafficking proteins are present throughout animal and plant lineages. However, very little is known about p24 developmental, spatial, or sex-specific expression patterns or how localized expression affects function. We investigated these problems in Drosophila melanogaster, which contains nine genes encoding p24 proteins. One of these genes, logjam (loj), is expressed in the adult female nervous system and ovaries and is essential for oviposition. Nervous system-specific expression of loj, but not ovary-specific expression, rescues the behavioral defect of mutants. The Loj protein localizes to punctate structures in the cellular cytoplasm. These structures colocalize with a marker specific to the intermediate compartment and cis-Golgi, consistent with experimental evidence from other systems suggesting that p24 proteins function in intracellular transport between the endoplasmic reticulum and Golgi. Our findings reveal that Drosophila p24 transcripts are developmentally and tissue-specifically expressed. CG31787 is male-specifically expressed gene that is present during the larval, pupal, and adult stages. Female CG9053 mRNA is limited to the head, whereas males express this gene widely. Together, our studies provide experimental evidence indicating that some p24 genes have sex-specific expression patterns and tissue-and sex-limited functions. Developmental Dynamics 236:544 -555, 2007.

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Three new Drosophila markers of intracellular membranes

Cited by 59 publications

References 10 publications

GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis

GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis

Sugar-free frosting, a homolog of SAD kinase, drives neural-specific glycan expression in the Drosophila embryo

Drosophila melanogasterp24 genes have developmental, tissue‐specific, and sex‐specific expression patterns and functions

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