Three new diterpenoids from the resin of<i>Liquidambar formosana</i>

Shang, Hong-Jie; Li, Dan-Yi; Wang, Wenjing; Li, Zhan‐Lin; Hua, Hui‐Ming

doi:10.1080/14786419.2013.825915

Cited by 21 publications

(12 citation statements)

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“…The approaches described could be useful, but the dregs in a decoction are usually discarded before consumption. Authentication is also possible by chemical or physical analyses such as high performance liquid chromatography [ 11 ], mass spectrometry [ 12 ] and nuclear magnetic resonance [ 13 ]. However, the chemical constituents in decoction may be altered by physiological conditions, harvesting period, storage and boiling conditions of the herbs involved.…”

Section: Introductionmentioning

confidence: 99%

Identification of constituent herbs in ginseng decoctions by DNA markers

Shaw

2015

Chin Med

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BackgroundDNA in herbal decoctions is usually fragmented by extensive boiling and is usually regarded as unsuitable for molecular authentication. This study aims to evaluate the feasibility for molecular authentication methods by multiplex polymerase chain reaction (PCR) and DNA sequencing for decoctions.MethodsThe DNA extraction procedure, sample pulverization and boiling time were examined in (1) single herb decoction with Panax ginseng (“ginseng”) or P. quinquefolius (“American ginseng”), (2) decoctions of two classical ginseng prescriptions of five herbal materials (Aconitum carmichaeli, Atractylodes macrocephala, P. ginseng, Glycyrrhiza uralensis and Zingiber officinale) and (3) a commercial ready-to-serve ginseng soup. Primers were designed from 26S-18S, ITS2 and trnH-psbA region, with DNA sequences obtained from GenBank. Multiplex PCR was also employed in ginseng or American ginseng decoctions to differentiate between these two herbs.ResultsAll six herbal species tested in this study could be identified in decoctions. We had four main observations: (1) sample pulverization before boiling improved PCR detection; (2) prolonged boiling increased the DNA concentration but decreased the intactness of DNA fragments; (3) ginseng could be differentiated from American ginseng by multiplex PCR; (4) identification of individual herbs in multi-herb decoctions with prolonged boiling time of 180 min could be achieved.ConclusionsDNA could be amplified from extensively boiled ginseng decoctions, multi-herb decoctions and commercial soup although DNA degradation was critical to successful PCR.Electronic supplementary materialThe online version of this article (doi:10.1186/s13020-015-0029-x) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Identification of constituent herbs in ginseng decoctions by DNA markers

Shaw

2015

Chin Med

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“…[ 4 ] Supportively, in our recent study, we also determined that the flavonoid amount of A. vulgaris is much higher in the methanol than the aqueous extract and as analysed by HPLC-DAD both extracts contained quercetin and gallic acid as the major vasoactive constituents. [ 10 ] Of these constituents, quercetin was demonstrated to induce relaxations[ 11 ] whereas; gallic acid was reported to produce contractions in isolated rat aorta. [ 20 ] Indeed, all these previous data are supporting our current findings in rat mesenteric arteries.…”

Section: Discussionmentioning

confidence: 99%

“…The major vasoactive constituents in methanol and aqueous extracts of Alchemilla vulgaris were previously determined by HPLC-DAD analysis. [ 10 ]…”

Section: Methodsmentioning

confidence: 99%

“…In relation, total flavonoid and quercetin amount was found much higher in the methanol extract while gallic acid in the aqueous extracts. [ 10 ] Quercetin, the most abundant flavonoid in medicinal plants, also in A. vulgaris , was demonstrated to improve endothelium-dependent relaxation and reduce the contractile responses in rat thoracic aortas. [ 11 12 ] It was suggested to be more potent in mesenteric vascular bed compared to aorta[ 13 ] and decrease the elevated blood pressure noticeably when given orally.…”

Section: Introductionmentioning

confidence: 99%

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Vasorelaxant and blood pressure lowering effects of alchemilla vulgaris: A comparative study of methanol and aqueous extracts

et al. 2015

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Background:In the last decade, a growing interest particularly in determining the cardiovascular effects of herbal extracts took place among researchers. Objective: Herein, we aimed to investigate the microvascular and blood pressure lowering effects of two differently processed extracts of the same herb, Alchemilla vulgaris (Rosaceaea), which was revealed to contain high levels of vasoactive compounds.Materials and Methods:For the purpose, endothelium intact rat mesenteric arteries were mounted in a myograph system and contracted with prostaglandin F2α (PGF2α: 3 × 10−5 M) or potassium chloride (K+: 40 mM). Then, aqueous and methanol extracts were added at 0.01–10 mg/ml concentrations in a cumulative manner.Results:Both extracts produced relaxations in PGF2α (3 × 10−5 M) precontracted arteries which were insensitive to the inhibitors of endothelium derived vasoactive substances namely, LG-nitro-L-arginine (10−4 M), ODQ (10−5 M) and indomethacin (10−5 M) or removal of endothelium. Opposite vascular effects were observed when extracts were applied in K+ precontracted arteries. In addition, oral administration of the methanol extract of Alchemilla vulgaris, but not the aqueous extract, reduced blood pressure significantly in L-NAME hypertensive rats.Conclusion:Our results demonstrated that the methanol extract of Alchemilla vulgaris has more prominent and favourable vascular effects in normal and experimental hypertensive conditions reinforcing its traditional use in cardiovascular disorders, in particular hypertension. These results most likely give rise to further studies to reveal its mechanism of action and clinical value of this herb.

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“…Several types of phytoconstituents have been isolated namely; tannins, [3][4][5] iridoids, 6 flavonoids, 7,8 di-and triterpenoids. [9][10][11][12][13] Reports on the biological activities were concerned with wound healing, 14 anti-ulcerogenic, 15 antifungal, 16 antimicrobial and antioxidant 17,18 effects.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective and antioxidant polyphenols from a standardized methanolic extract of the leaves of Liquidambar styraciflua L.

Eid¹,

Labib²,

Hamid³

et al. 2015

Bulletin of Faculty of Pharmacy, Cairo University

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The methanolic extract of the leaves of Liquidambar styraciflua L. (Altingiaceae) (LSE) was evaluated for hepatoprotective and antioxidant activities in carbon tetrachloride liver-damaged rats. Hepatotoxicity was induced via intraperitoneal injection of CCl 4 1:9 in olive oil, at a dose of 0.5 ml/kg b.wt. The animals received the extract, orally, at two dose levels (250 and 500 mg/kg b.wt.) The administration regimen was twice a week, for six consecutive weeks. LSE exhibited a significant dose-dependent protective effect by lowering the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), malondialdehyde (MDA) and ameliorating the level of serum protein. In addition, LSE showed antioxidant activity through improving the levels of blood glutathione (GSH), vitamin C, vitamin E and hepatic total protein contents. The LSE revealed activity approached that of silymarin, a known hepatoprotective agent. These biochemical observations were supported by examination of the histopathological features of the liver. Chromatographic fractionation of LSE afforded seven phenolic compounds. These were identified on the basis of chromatographic, chemical and spectroscopic analyses as: gallic acid (1), isorugosin B (2), casuarictin (3), quercetin-3-O-b-D-4 C 1 -glucopyranoside (4), myricetin-3-O-a-L-1 C 4 -rhamnopyranoside (myricetrin) (5), quercetin (6) and myricetin (7). The isolated phenolics probably account for the antioxidant and hepatoprotective effects exhibited by the parent extract. Furthermore, a validated RP-HPLC method was devised for standardization of LSE, in view to fulfill the requirements of efficient research methodology for evaluation of bioactive herbal drugs.The above findings suggested that LSE could be considered as a standardized herbal product with antifibrotic, hepatoprotective and antioxidant potential.ª 2015 Production and hosting by Elsevier B.V. on behalf

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Three new diterpenoids from the resin ofLiquidambar formosana

Abstract: Three new diterpenoids, liquidambolide A (1) and liquiditerpenoic acids A (2) and B (3), together with 10 known diterpenes were isolated from the resin of Liquidambar formosana Hance, whose structures were elucidated by detailed analysis on the NMR and HR-ESI-MS spectra.

Cited by 21 publications

References 13 publications

Identification of constituent herbs in ginseng decoctions by DNA markers

Identification of constituent herbs in ginseng decoctions by DNA markers

Vasorelaxant and blood pressure lowering effects of alchemilla vulgaris: A comparative study of methanol and aqueous extracts

Hepatoprotective and antioxidant polyphenols from a standardized methanolic extract of the leaves of Liquidambar styraciflua L.

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