Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation

Abstract

In this study, we identified three neuroparsin (NP) genes in Macrobrachium nipponense: Mn-NP1, Mn-NP2, and Mn-NP3, encoding 99, 100, and 101 amino acid proteins, respectively. Multiple sequence alignments showed that these genes contained 12 cysteine residues, of which 11 were at conserved positions. The total sequence identity between the genes was 47.5%, and they showed a high degree of sequence identity (> 54% similarity) with other crustacean genes. Phylogenetic tree analysis showed that Mn-NPs were clustered at different branches, indicating that Mn-NPs may have different functions. Tissue distribution data revealed that the three genes were present in males and females during the breeding and nonbreeding season, but their expression patterns differed. Mn-NP1 was highly expressed in the breeding season, in the male testis, and highly expressed in the nonbreeding season, in the female ovary. Mn-NP3 exhibited biased female expression in the breeding and nonbreeding season, with dominant expression in the ovary. All Mn-NPs were detected during embryo development, but with different expression patterns. These data indicated that Mn-NP1 may function during embryonic development, and that Mn-NP2 may be expressed during early embryo cell division, and late larval development. Mn-NP3 expression patterns reflected maternal inheritance, and may be associated with ovarian maturation. These expression data suggested that Mn-NP1 and Mn-NP2 are negatively correlated with ovarian development, with inhibition roles during this development. Mn-NP3 may be involved in vitellogenesis.

Identification of Neuropeptides Using Long-Read RNA-Seq in the Swimming Crab Portunus trituberculatus, and Their Expression Profile Under Acute Ammonia Stress

“…As shown in Figures 2B–K , highly conserved motifs were identified in a number of neuropeptide families, such as AST-B (XWXGXWamide), FLRFamide (R/K-N/S-F/Y-LRFamide), MYO (QDLDHVFLRFamide), RPCH (pQLNFSPGWamide), sNPF (XPXXRLRFamide), and family of CHH and NP (conserved Cys) ( Backing et al, 2002 ; Christie et al, 2010 ; Christie 2014 ; Nguyen et al, 2016 ; Liu et al, 2020 ; Qiao et al, 2020 ). In addition, the amino acid sequences of all the putative neuropeptide precursor and their structure information, including sites of bioactive mature peptides, location of cleavage sites, and precursor size were provided in Supplementary File S1, S2 .…”

Neuroparsin 1 (MrNP1) and Neuroparsin 2 (MrNP2) Are Involved in the Regulation of Vitellogenesis in the Shrimp Macrobrachium rosenbergii

“…Three different NPs were identified in M. nipponense (Qiao et al, 2020). Despite that M. rosenbergii MrNP1 and MrNP2 share only 56% amino identity, the comparison between M. rosenbergii MrNP1 and M. nipponense MnNP3 and the comparison between M. rosenbergii MrNP2 and M. nipponense MnNP1 show high degrees of amino acid sequence identity (97%), which suggests that they should have a strong structure-function relationship.…”

Function Analysis of Cholesterol 7-Desaturase in Ovarian Maturation and Molting in Macrobrachium nipponense: Providing Evidence for Reproductive Molting Progress

“…It seriously affects the production and economic benefit of shrimp farming [ 7 ]. In response to this problem, the research team screened many genes related to rapid sexual maturity [ 8 , 9 ]. In the hepatopancreas transcriptome of female M. nipponense adult ovaries from the O-I to O-V stages of development, KEGG enrichment revealed that the “insect hormone synthesis” signaling pathway was closely related to ovarian development.…”