“…The opportunity of defining precise regions of the herpes simplex virus (HSV) genome involved in pathogenicity has been provided by the isolation of deletion variants of both HSV-1 and -2 (Brown et al, 1984;Harland & Brown, 1985Brown & Harland 1987;MacLean & Brown, 1987a, b;Efstathiou et al, 1989). We have recently shown that the JH2604 deletion variant of HSV-2 strain HG52 (Harland & Brown, 1985) is avirulent when inoculated intracerebrally into mice, with an LDso value of > 107 p.f.u./mouse compared to < 102 p.f.u./ mouse for the parental virus (Taha et al, 1989a).…”