Three-Input Logic Gates with Potential Applications for Neuronal Imaging

Abstract

Convenient methods for the direct visualization of neurotransmitter trafficking would bolster investigations into the development of neurodegenerative diseases. Here, tunable fluorescent molecular logic gates with applications to neuronal imaging have been developed. The three-input AND molecular logic gates are based on the coumarin-3-aldehyde scaffold and designed to give a turn-on fluorescence response upon the corelease of glutamate and zinc from secretory vesicles via exocytosis. Spectroscopic studies reveal an 11-fold fluorescence enhancement under conditions mimicking exocytosis. Methylation of the scaffold was used to optimize the spectral profile of the sensors toward desired excitation wavelengths. A binding study that elucidates the sensor-analyte interactions is presented. These sensors serve as a proof-of-concept toward the direct imaging of neurotransmitters released upon exocytosis using fluorescent molecular logic gates.

Quantum chemical elucidation of the turn-on luminescence mechanism in two new Schiff bases as selective chemosensors of Zn2+: synthesis, theory and bioimaging applications

“…[22][23][24] Therefore, the design of effective and sensitive Zn 2+ probe, especially with high water-solubility, uorescent enhancements, and a simple synthesis method, has become crucially important. [25][26][27][28] In this work, we report two selective zinc sensors (A and B) based on a Schiff base, and an amino acid (glutamine), to improve the solubility and biocompatibility of the molecules for possible biological assays.…”

Intelligent Molecular Searcher from Logic Computing Network Based on Eu(III) Functionalized UMOFs for Environmental Monitoring

“…It agrees well with the chromaticity coordinates calculated from the emission spectra (Figure S4, Supporting Information). Fluorescence‐based powerful signal transducer for logic outputs exhibits immense importance because they can be used to solve complex problems on the molecular level . In addition, the dual‐emission fluorescence materials in our case will provide the possibility to design multiple‐configurable logic gates for more applications.…”

An Elaborate Supramolecular Assembly for a Smart Nanodevice for Ratiometric Molecular Recognition and Logic Gates

“…Formation of logic gates using changes in optical responses is important because they can be used to solve complex problems on the molecular level. [24] The CDots@SC4A-Tb 3 + nanodevice can be used as af luorescence logic gateb yu sing CDots@SC4A,T b 3 + ,a nd H 2 PO 4 À as the chemical inputs. As shown in Figure 4a,t he fluorescentc hange can be described and manipulateda salogic gate by at hreshold of CDots@S-C4 A,T b 3 + and H 2 PO 4 À .T he absence and presence of these ions were defined as the 0a nd 1s tates,r espectively.T he eight possible input combinations are shown in the truth table in Figure 4b.T his system can form two different patterns of logic gate at the two different wavelengths.…”