Three Heat Shock Protein Genes and Antioxidant Enzymes Protect Pardosa pseudoannulata (Araneae: Lycosidae) from High Temperature Stress

Fu, Di; Liu, Jing; Pan, Ying-Na; Zhu, Jianwei; Xiao, Feng; Liu, Min; Xiao, Rong

doi:10.3390/ijms232112821

Cited by 4 publications

(4 citation statements)

References 69 publications

(91 reference statements)

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“…With the increase in environmental temperature, the genetic diversity of P. pseudoannulata populations show a decreasing trend (Sun et al 2007). The expression levels of six heat‐shock protein genes in P. pseudoannulata are significantly upregulated under high‐temperature stress, compared with 25°C (Fu et al 2022; Sun et al 2021). Currently, some gene studies related to temperature stress in spiders have been published, but there is no research specifically focused on the miRNAs of spiders.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Zhang,

Wang,

Song

et al. 2024

Entomological Research

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MicroRNAs (miRNAs) are conserved noncoding small RNAs that play essential regulatory roles in gene function by regulating target genes. Pardosa pseudoannulata is an important natural predatory enemy of insect pests and plays a significant role in controlling pests in rice fields, with temperature having a significant impact on their growth and development. To understand the response of miRNAs to temperature stress in P. pseudoannulata, we performed miRNA identification analyses of adult spiders exposed to 10°C and 40°C for 12 h, as low‐temperature and high‐temperature treatment groups, respectively. We obtained 54.74 M clean reads from 69.84 M raw reads after filtering out low‐quality reads, and 78 miRNAs including 13 novel miRNAs were identified from three small RNA libraries (10°C, 25°C and 40°C). At the low temperature and the high temperature, eight (one upregulated and seven downregulated) and ten (nine upregulated and one downregulated) differentially expressed miRNAs were identified, respectively. These differentially expressed miRNAs negatively regulated 43 and 12 target mRNA (the unigenes in our previous transcriptome sequence data) in response to low‐ and high‐temperature stress, respectively. These target genes are mainly involved in translation, ribosome structure and biotransformation, as well as the generation and conversion of energy. This study represents the first report of miRNA identification related to the Araneae spider species in response to temperature stress. These results will greatly facilitate our understanding of the physiological and biochemical mechanisms of spiders in response to temperature stress, which might be beneficial for the conservation and utilization of this species as an important natural insect enemy of pests in rice ecosystems.

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Section: Introductionmentioning

confidence: 99%

MicroRNA response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Zhang,

Wang,

Song

et al. 2024

Entomological Research

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“…Especially in the current context of global warming, such research can provide important theoretical foundations for studying biological responses to high-temperature stress. Current research on spiders' response to high temperatures mainly concentrates on the expression of heat shock protein genes (Sun et al 2021) and the function of heat shock protein genes (Fu et al 2022). There is comparatively less research on the relationship between microRNAs (miRNAs) and temperature in P. pseudoannulata.…”

Section: Introductionmentioning

confidence: 99%

“…2021) and the function of heat shock protein genes (Fu et al . 2022). There is comparatively less research on the relationship between microRNAs (miRNAs) and temperature in P. pseudoannulata .…”

Section: Introductionmentioning

confidence: 99%

Expression characterization of miR‐6826‐3p in Pardosa pseudoannulata (Araneae: Lycosidae) under high temperature stress

Zhang,

Fu,

Yang

et al. 2024

Entomological Research

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Pardosa pseudoannulata is key predator in rice ecosystems; it plays a crucial role in rice pest biological control, effectively managing major rice pests such as rice planthopper. At present, there are few related studies on spiders' microRNA (miRNA). Consequently, we initiate a study on miR‐6826‐3p in P. pseudoannulata in response to high temperatures. First, we identified a total of 686 target genes of miR‐6826‐3p, 439 target genes had annotated results in the database, primarily involved in transcription and metabolism functions. Subsequently, quantitative real‐time PCR was employed to assess the relative expression levels of miR‐6826‐3p in P. pseudoannulata under different temperatures (31, 34, 37, 40°C) and various durations (1, 2, 4, 8, 12 h). The results revealed that compared to the control group (25°C), the relative expression level of miR‐6826‐3p in female spiders significantly increased under the same temperature and different duration stresses. The expression level of miR‐6826‐3p in male spiders showed an initial increase followed by a decrease trend, with relatively lower expression levels after 1 and 12 h at the same temperature conditions. Furthermore, under the same duration of stress, compared to the control group (25°C), the relative expression level of miR‐6826‐3p in female spiders significantly increased at different temperatures. These results demonstrate that P. pseudoannulata miR‐6826‐3p plays a significant role in response to high‐temperature stress. The research findings can provide a basis for future in‐depth studies on the miRNA functional of P. pseudoannulata, aiding in the elucidation of the regulatory mechanisms of P. pseudoannulata miRNAs in response to high‐temperature environments.

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“…The wolf spider Pardosa pseudoannulata (Araneae: Lycosidae), which is a hunting spider, 30 mainly feeds on rice pests such as planthoppers, leafhoppers, and other Lepidoptera insects. [31][32][33] It has strong adaptability to the environment and is widely distributed, [34][35][36][37][38] generally being found in wet habitats near the edge of water bodies. 39 Research has shown that the vitellogenin receptor gene 38 and the functional ecdysteroid Ponasterone A (PA) 40 are vital to the growth and development of Pardosa pseudoannulata.…”

Section: Introductionmentioning

confidence: 99%

RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata

Li,

Zhang

et al. 2023

Pest Management Science

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BACKGROUNDInsulin signalling pathways play crucial roles in regulating growth and development in insects, but their effects on the growth and development of Arachnids, such as spiders, have rarely been studied. As a valuable pest natural enemy in agricultural fields, the molecular mechanisms of insulin signalling pathway‐mediated growth and development of the wolf spider, Pardosa pseudoannulata, are of particular interest.RESULTSIn this study, we identified and characterized six insulin signalling pathway genes—InR, InR2, IRS1, PI3K1, PI3K2, and PDK—in P. pseudoannulata. Real‐time quantitative PCR results were used to analyse the relative expression levels of the six genes in different developmental instars and tissues, and in response to starvation treatment. In addition, the function of the insulin receptor substrate (IRS1) gene was investigated using RNA interference technology, which found that IRS1 significantly influenced nutrient content, developmental duration, body weight, and gonad development.CONCLUSIONThis study revealed the roles of six key insulin signalling pathway genes in P. pseudoannulata, and in particular the importance of the IRS1 gene in regulating growth and development in the spider. The results lay the foundation for further research on the internal regulation mechanisms of growth and development in Araneae species, and also provide a reference for the artificial breeding of spiders.This article is protected by copyright. All rights reserved.

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Three Heat Shock Protein Genes and Antioxidant Enzymes Protect Pardosa pseudoannulata (Araneae: Lycosidae) from High Temperature Stress

Cited by 4 publications

References 69 publications

MicroRNA response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

MicroRNA response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Expression characterization of miR‐6826‐3p in Pardosa pseudoannulata (Araneae: Lycosidae) under high temperature stress

RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata

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