“…These genes included two vitellogenins ( Vd Vg1, Vd Vg2) [33], a large lipid transfer protein ( Vd LLTP) [34], foraging ( Vd For) and malvolio ( Vd Mvl) [60], spook ( Vd spo), disembodied ( Vd dib), shade ( Vd shd) [59] and a co-transcription factor subolesin ( Vd sub) (Cabrera and Shirk, unpublished data). Comparison of the transcript levels for each of the nine selected genes between untreated control phoretic mites and the phoretic mites that survived the feeding protocol showed that that there were no significant differences after completing the feeding protocol (VdLLTP: t = -1.13, p = 0.3228, n = 3; VdVg1: t = 1.97, p = 0.1195, n = 3; VdVg2: t = 1.12, p = 0.3260, n = 3; VdFor: t = -1.20, p = 0.2964, n = 3; VdMvl: t = -1.87, p = 0.1341, n = 3; Vdspo: t = -1.35, p = 0.2475, n = 3; Vddib: t = -1.70, p = 0.1640, n = 3; Vdshd: t = -1.12, p = 0.3274, n = 3; Vdshd: t = -1.12, p = 0.3247; Vdsub: t = -0.84, p = 0.4468, n = 3); Fig 4).…”